Keywords
Clinical Laboratory Techniques
Influenza Human
How to Cite
Abstract
This study standardized a multiplex RT-qPCR protocol for detecting Influenza A virus in formalin-fixed, paraffinembedded (FFPE) tissues from suspected Severe Acute Respiratory Syndrome (SARS) cases. A total of 174 samples were analyzed with an adapted extraction method and a commercial respiratory virus assay. Influenza A was detected in 4.6% of cases, along with RSV, Influenza B, and SARS-CoV-2. The method proved feasible and robust for differential diagnosis in FFPE tissues, complementing immunohistochemistry. Multiplex RT-qPCR in post-mortem samples shows strong potential to improve etiological surveillance of fatal cases and support evidence-based public health decisions.
References
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Copyright (c) 2025 Camila Santos da Silva Ferreira, Isabelle Dias de Oliveira, Cinthya dos Santos Cirqueira Borges, Juliana Mariotti Guerra, Leonardo José Tadeu de Araújo