Keywords
Hanseniasis
Immune complexes
C1q
Immune complexes
C1q
How to Cite
1.
KLEEMANN TAE, FERRI RG, CHAVES J, IRULEGUI I, COPPI VAZ CA. Detecção de complexos imunes circulantes na hanseníase utilizando C1q humano e eqüino. Hansen. Int. [Internet]. 1978 Nov. 30 [cited 2025 Oct. 19];3(2):135-40. Available from: https://periodicos.saude.sp.gov.br/hansenologia/article/view/36079
Abstract
Presence of soluble immune complexes (IC) was observed in sera of Virchowian hanseniasis patients; some of these patients presented active erythema nodosum. These IC were detected by their reactivity with the C,q component of the complement. The IC were previously precipitated by polyethylene-glycol (PEG) and dissolved in borate buffer. The presence of IC was confirmed by reduction with 2-mercapto-ethanol and alqullation with 2 iodoacetamide followed by new C,q reactions and no precipitin lines were obtained.
References
1. AGNELLO, V.; GABRIEL JR, A.; MINDY TAI, B.A. Detection of immune complexes. J. Invest. Derm., 67(3) :339-345, 1976.
2. AGNELLO, V.; WINCHESTER, R.J.; KUNKEL, H.G. Precipitin reactions of the Clq component of complement with aggregated gamma-globulin and immune complexes in gel diffusion. Immunology, 19:909-919, 1970.
3. BONOMO, L. & DAMMACCO, F. Immune complex cryoglobulinaemia in lepromatous leprosy: a pathogenetic approach to some clinical features of leprosy. Clin. Exp. Immunol., 9:175-181, 1971.
4. BOUT, D.; SANTORO, F.; CARLIER, Y.; CAPRON, A. Imuno-complexos na esquistossomose. III. Caracterização das imunoglobulinas e dos antígenos implicados no IC. Rev. Inst. Med. Trop. São Paulo, 19(1) :35-38, 1977.
5. CHAVES, J.; FERRI, R.G.; KLIEMANN, T.A.E.; IRULEGUI, I. Complexos imunes circulantes na doença de Chagas experimental. Identificação de antígenos parasitários nos complexos. [No prelo]
6. EWALD, R.W. & SCHUBART, A.F. Agglutinating activity of the complement component C',q in the F-II latex fixation test. J. Immunol., 97(1):100-106, 1966.
7. GELBER, R.H.; EPSTEIN, W.V.; FASAL, P.; DRUTZ, D.J. Detection of circulating immune complex-like activity in the sera of patients with erythema nodosum leprosum. In: LEPROSY RESEARCH CONFERENCE, 7th, Menlo Park Ca., 1972. Program apud Int. J. Lepr., 40(2):218-219, 1972.
8. JUNQUEIRA, L.C.; BIGNOLAS, G.; FERRI, R.G.; CHAVES, J.; KLIEMANN, T.A.E. Staining of Clq protein from human and horse complement with dye Sirius Red. [No prelo]
9. MORAN, C.J.; RYDER, G.; TURK, J.L.; WATERS, M.F.R. Evidence for circulating immune complexes in lepromatous leprosy. Lancet, 2(7777): 572-573, 1972.
10. MULLER EBERHARD, H.J. & KUNKEL, H.G. Isolation of a thermolabile serum protein which precipitates gamma-globulin aggregates and participates in immune hemolysis. Proc. Soc. Exp. Biol, Med., 106(2):291-295, 1961.
11. ROJAS-ESPINOSA, O.; MENDEZ- NAVARRETE, I.; ESTRADA-PARRA, S. Presence of Clq-reactive immune complexes in patients with leprosy. Clin. Exp. Immunol., 12:215-223, 1972.
12. WEMAMBU, S.N.C.; TURK, T.L.; WATERS, M.F.R., REES, R.J.W. Erythema nodosum leprosum: a clinical manifestation of the Arthus phenomenon. Lancet, 2:933-935, 1969.
13. WINCHESTER, R.J. & AGNELLO, V. Detection of immune complexes by direct precipitation with C,q or IBM rheumatoid factors. Ann. Rheum. Dis., 36(suppl.) :35-36. 1977.
14. ZUBLER, R.H.; PERRIN, L:H.; CREIGHTON, W.D.; LAMBERT, P.H. Use of polyethilene glycol (PEG) to concentrate immune complexes from serum or plasma samples. Ann. Rheum. Dis., 36(suppl.) :23-25, 1977.
2. AGNELLO, V.; WINCHESTER, R.J.; KUNKEL, H.G. Precipitin reactions of the Clq component of complement with aggregated gamma-globulin and immune complexes in gel diffusion. Immunology, 19:909-919, 1970.
3. BONOMO, L. & DAMMACCO, F. Immune complex cryoglobulinaemia in lepromatous leprosy: a pathogenetic approach to some clinical features of leprosy. Clin. Exp. Immunol., 9:175-181, 1971.
4. BOUT, D.; SANTORO, F.; CARLIER, Y.; CAPRON, A. Imuno-complexos na esquistossomose. III. Caracterização das imunoglobulinas e dos antígenos implicados no IC. Rev. Inst. Med. Trop. São Paulo, 19(1) :35-38, 1977.
5. CHAVES, J.; FERRI, R.G.; KLIEMANN, T.A.E.; IRULEGUI, I. Complexos imunes circulantes na doença de Chagas experimental. Identificação de antígenos parasitários nos complexos. [No prelo]
6. EWALD, R.W. & SCHUBART, A.F. Agglutinating activity of the complement component C',q in the F-II latex fixation test. J. Immunol., 97(1):100-106, 1966.
7. GELBER, R.H.; EPSTEIN, W.V.; FASAL, P.; DRUTZ, D.J. Detection of circulating immune complex-like activity in the sera of patients with erythema nodosum leprosum. In: LEPROSY RESEARCH CONFERENCE, 7th, Menlo Park Ca., 1972. Program apud Int. J. Lepr., 40(2):218-219, 1972.
8. JUNQUEIRA, L.C.; BIGNOLAS, G.; FERRI, R.G.; CHAVES, J.; KLIEMANN, T.A.E. Staining of Clq protein from human and horse complement with dye Sirius Red. [No prelo]
9. MORAN, C.J.; RYDER, G.; TURK, J.L.; WATERS, M.F.R. Evidence for circulating immune complexes in lepromatous leprosy. Lancet, 2(7777): 572-573, 1972.
10. MULLER EBERHARD, H.J. & KUNKEL, H.G. Isolation of a thermolabile serum protein which precipitates gamma-globulin aggregates and participates in immune hemolysis. Proc. Soc. Exp. Biol, Med., 106(2):291-295, 1961.
11. ROJAS-ESPINOSA, O.; MENDEZ- NAVARRETE, I.; ESTRADA-PARRA, S. Presence of Clq-reactive immune complexes in patients with leprosy. Clin. Exp. Immunol., 12:215-223, 1972.
12. WEMAMBU, S.N.C.; TURK, T.L.; WATERS, M.F.R., REES, R.J.W. Erythema nodosum leprosum: a clinical manifestation of the Arthus phenomenon. Lancet, 2:933-935, 1969.
13. WINCHESTER, R.J. & AGNELLO, V. Detection of immune complexes by direct precipitation with C,q or IBM rheumatoid factors. Ann. Rheum. Dis., 36(suppl.) :35-36. 1977.
14. ZUBLER, R.H.; PERRIN, L:H.; CREIGHTON, W.D.; LAMBERT, P.H. Use of polyethilene glycol (PEG) to concentrate immune complexes from serum or plasma samples. Ann. Rheum. Dis., 36(suppl.) :23-25, 1977.
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