Método de diagnóstico antemortem da raiva humana por meio de técnicas de biologia molecular, utilizando saliva e biópsia de pele da região da nuca
Palabras clave:
Raiva, Diagnóstico Antemortem, Reação em cadeia da PolimeraseResumen
Por meio das técnicas de RT-PCR com primers direcionados para o gene da glicoproteína e RT-PCR e hemi-nested RT-PCR com primers direcionados para o gene da nucleoproteína, o RNA do vírus da raiva foi identificado em 95,2% de 21 amostras, 18 de saliva e três de biópsia de pele da região da nuca, coletadas entre a hospitalização e a morte de um paciente com sinais clínicos da raiva. O tratamento administrado ao paciente incluiu a indução de coma e terapia antiviral. Cada técnica, isoladamente, detectou RNA viral em 90,5%; 57,1% e 85,7% das amostras, respectivamente. Nossos resultados sugerem que a amplificação em paralelo de diferentes regiões do genoma do vírus da raiva pode fornecer maior confiabilidade ao diagnóstico antemortem da doença, auxiliando a decisão médica quanto à aplicação do protocolo de tratamento com antivirais.
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Derechos de autor 2013 Juliana Galera Castilho, Carla Isabel Macedo, Saulo de Oliveira Santos, Rafael de Novaes Oliveira, Samira Maria Achkar, Maria Luiza Carrieri, Ivanete Kotait, Pedro Carnieli Jr
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
