Palavras-chave
Bacillus cereus
Produtos Lácteos
Patogenicidade
Reação em Cadeia da Polimerase
Como Citar
Resumo
A qualidade de produtos derivados do leite foi verificada em 63 amostras, sendo 24 de sorvetes artesanais, 19 de cremes de leite pasteurizados e 20 de pâtés de queijo. As análises microbiológicas foram realizadas para averiguar a presença de coliformes termotolerantes, estafilococos coagulase positiva, Salmonella spp., bolores e leveduras, Bacillus cereus e Listeria monocytogenes. Além disso, foram realizados ensaios para efetuar a caracterização do potencial enterotoxigênico de B. cereus quanto aos genes codificadores das enterotoxinas NHE e HBL por meio de técnica PCR e a subtipagem molecular dos genes actA e hly deL. monocytogenes. As 33% (8/24) de amostras de sorvetes e 15,8% (3/19) de amostras de cremes de leite estavam em desacordo com os padrões microbiológicos estabelecidos para coliformes termotolerantes, de acordo com a RDC Nº12/2001 da ANVISA. A presença de L. monocytogenes foi observada em apenas uma amostra de creme de leite e os isolados da bactéria foram identificados como pertencentes à linhagem I, que é potencialmente patogênica ao homem. B. cereus com potencial enterotoxigênico foi isolado nos três grupos de alimentos estudados. O perfil de contaminação microbiológica observado nos produtos avaliados neste estudo serve de alerta para que os produtores melhorem as condições higiênico-sanitárias no processamento e da necessidade das autoridades sanitárias em estabelecer maior controle desses produtos.
Referências
1. De Buyser ML, Dufour B, Marie M, Lafarge V. Implication of milk and milk products in food-borne disease in France and different industrialized countries. Int J Food Microbiol 2001; 67 (1-2): 1-17.
2. Germano PML, Germano MIS. Higiene e Vigilância Sanitária de Alimentos. São Paulo: Varela, 2001.
3. Henessy TW, Hedberg CW, Slutsker L, White KE, Besser-Wiek JM, Moen ME, Feldman J, Coleman WW, Edmonson LM, MacDonald KL, Osterholm MT. A national outbreak of Salmonella enteritidisinfections from ice cream. N Engl J Med 1996;334 (20): 1281-6.
4. Djuretic T, Wall PG, Nichols G. General outbreaks of infectious intestinal disease associated with milk and dairy products in England and Wales: 1992 to 1996. Commun Dis Rep CDR Wkly 1997; 7: 41-5.
5. Dodhia H, Kearney J, Warburton F. A birthday party, home-made ice cream, and an outbreak of Salmonella enteritidis pahge type 6 infection. Commun Dis Public Health 1998; 1: 31-4.
6. Armondes MP, Issy PN, André MCDPB, Serafi ni AB. Aspectos higiênico-sanitários de sorvetes produzidos artesanalmente na cidade de Goiânia, GO. Hig Alim 2003; 17 (107): 86-94.
7. De Farias FF, Da Silva WR, Botelho ACN, Da Hora IMC, Kronenberger G, Da Cruz AG. Microbiological quality of ice creams commercialized in some cities in the state of Rio de Janeiro, Brazil. Int J Dairy Technol 2006;59 (4): 261-4.
8. El-Sharef N, Ghenghesh KS, Abognah YS, Gnan SO, Rahouma A. Bacteriological quality of ice cream in Tripoli-Libya. Food Control 2006; 17: 637-41.
9. Kanbakan U, Çon AH, Ayar A. Determination of microbiological contamination sources during ice cream production in Denizli, Turkey. Food Control 2004; 15: 463-70.
10. André MCDPB, Campos MRH, Borges LJ, Kipnis A, Pimenta FC, Serafi ni AB. Comparison of Staphylococcus aureus isolates from food handlers, raw bovine milk and Minas Frescal cheese by antibiogram and pulsed-fi eld gel electrophoresis following SmaI digestion. Food Control 2008; 19: 200-07.
11. Carmo LS, Dias RS, Linardi VR, Sena MJ, Santos DA, Faria ME, Pena EC, Jett M, Heneine LG. Food poisoning due to enterotoxigenic strains of Staphylococcus present in Minas cheese and raw milk in Brazil. Food Microbiol 2002;19: 9-14.
12. Franco BDGM, Landgraf M. Microbiologia dos Alimentos. São Paulo: Atheneu, 2005.
13. Hansen BM, Hendriksen NB. Detection of Enterotoxic Bacillus cereus and Bacillus thuringiensis Strains by PCR Analysis. Appl Environ Microbiol 2001; 67: 185-9.
14. Rosenquist H, Smidt L, Andersen SR, Jensen GB, Wilcks A. Occurrence and signifi cance of Bacillus cereus and Bacillus thuringiensis in ready-to-eat food. FEMS Microbiol Lett 2005; 250: 129-36.
15. Wijnands LM, Dufrenne JB, Rombouts FM, Veld PH, Leusden FM. Prevalence of potentially pathogenic Bacillus cereus in food commodities in the Netherlands. J Food Prot 2006; 69 (11):2587-94.
16. Wong TL, Carey-Smith GV, Hollis L, Hudson JA. Microbiological survey of prepackaged pâté and ham in New Zealand. Lett Appl Microbiol 2005;41: 106-11.
17. Arnensen LPS, O ́Sullivan K, Granum PE. Food poisoning potential of Bacillus cereus strains from Norwegian dairies. Int J Food Microbiol 2007; 116: 292-6.
18. Bartoszewicz M, Hansen BM, Swiecicka I. The members of Bacillus cereus group are commonly present contaminants of fresh and heat-treated milk. Food Microbiol 2008; 25: 588-96.
19. Svensson B, Monthán A, Guinebretière MH, Nguyen-Thé C, Christiansson A. Toxin production potential and the detection of toxin genes among strains of the Bacillus cereus group isolated along the dairy production chain. Int Dairy J 2007;17: 1201-08.
20. Zhou G, Liu H, He J, Yuan Y, Yuan Z. The occurrence of Bacillus cereus, B. thuringiensis and B. mycoides in Chinese pasteurized full fat milk. Int J Food Microbiol 2008; 121: 195-200.
21. Aygun O, Pehlivanlar S. Listeria spp. in the raw milk and dairy products in Antakya, Turkey. Food Control 2006; 17: 676-9.
22. Colak H, Hampikyan H, Bingol EB, Ulusoy B. Prevalence of L. monocytogenes and Salmonella spp. in Tulum cheese. Food Control 2007; 18: 576-9.
23. Kabuki DY, Kuaye AY, Wiedmann M, Boor KJ. Molecular subtyping and tracking of Listeria monocytogenes in latin-style fresh-cheese processing plants. J Dairy Sci 2004; 87: 2803-12.
24. Kalorey DR, Warke SR, Kurkure NV, Rawool DB, Barbuddhe SB. Listeria species in bovine raw milk: A large survey of Central India. Food Control 2008; 19: 109-12.
25. Wiedmann M, Bruce JL, Keating C, Johnson AE, McDounough PL, Batt CA. Ribotypes and virulence gene polymorphisms suggest three distinct Listeria monocytogenes lineages with differences in pathogenic potential. Infect Immun 1997; 65 (7): 2707-16.
26. Brasil. Ministério da Saúde. Agência Nacional de Vigilância Sanitária (ANVISA). Resolução RDC n° 12 de 02 de janeiro de 2001. Aprova regulamento técnico sobre padrões microbiológicos em alimentos. Diário Ofi cial [da] republica federativa do Brasil. Brasília, DF, 10 de jan. 2001, Seção 1.
27. Downes FP, Ito K. Compendiumof methods for the microbiological examination of foods. 4th edition, Washington: American Public Health Association, 2001, 676p.
28. Pagotto F, Daley E, Farber J. Warburton, D. Isolation of Listeria monocytogenes from all food and environmental samples. In: CANADA. Health Products Food Branch. Compendium of analytical methods: laboratory procedures of microbiologicalanalytical of foods, [MFHPB 30] Ottawa, 2001. Available from: http://www.hc-sc.gc.ca
29. Kabuki DY, Soares CM, Souza RM, Kuaye AY. Avaliação do perfi l enterotoxigênico de Bacillus cereus isolados de queijos Minas frescal. XX Congresso Brasileiro de Ciência e Tecnologia de Alimentos, Curitiba, PR, 2006.
30. Jo C, Kim H-J, Kim D-H, Lee W-K, Ham, J-S, Byun, M-W. Radiation sensitivity of selected pathogens in ice cream. Food Control 2007; 18: 859-65.
31. Warke R, Kamat A, Kamat M, Thomas P. Incidence of pathogenic psychrotrophs in ice creams sold in some retail outlets in Mumbai, India. Food Control 2000; 11: 77-83.
32. Kramer JM, Gilbert RJ. Bacillus cereus and other Bacillus species. In: Doyle MP, ed. Foodborne bacterial pathogens. New York: Marcel Dekker, 1989, p. 21-69.
33. Esper LMR. Diagnóstico da qualidade de ricotas comercializadas no município de Campinas - SP. [dissertação] Campinas, São Paulo: Universidade Estadual de Campinas (UNICAMP); 2006.
34. Kabuki DY, Rocha JAK, Kuaye AY. Ocorrência de Bacillus cereus em queijos Minas frescal elaborados por diferentes processos tecnológicos. XXIII Congresso Brasileiro de Microbiologia, Santos, SP, 2005.
35. Granum PE. Bacillus cereus. In M. P. Doyle (Ed.), Food microbiology: Fundamentals and frontiers. Washington, DC, USA: ASM Press, p.373-81, 2001.
36. Beecher DJ, Schoeni JL, Wong ACL. Enterotoxic activity of hemolysin BL from Bacillus cereus. Infect Immun 1995; 63(11): 4423-8.
37. Schoeni JL, Wong ACL. Bacillus cereus food poisoning and its toxins. J Food Prot 2005; 68(3): 636-48.
38. Guinebretière MH, Nguyen-The C. Sources of Bacillus cereuscontamination in a pasteurized zucchini purée processing line, differentiated by two PCR-based methods. FEMS Microbiol Ecol 2003; 43(2): 207-15.
39. Nichols G, McLauchlin J, Louvois J. The contamination of pâté with Listeria monocytogenes. Results from the 1994 European Community – Coordinated Food Control Program for England and Wales. J Food Prot 1998; 61(10): 1299-1304.
40. Dominguez C, Gomez I, Zumalacarregui J. Prevalence and contamination levels of Listeria monocytogenes in smoked fi sh and pâté sold in Spain. J Food Prot 2001; 64 (12): 2075-7.
41. Kiss R, Tircza T, Szita G, Bernath S, Csikó G. Listeria monocytogenesfood monitoring data and incidence of human listeriois in Hungary. Int J Food Microbiol 2004; 112: 71-4.
42. Swaminathan B, Gerner-Smidt P. The epidemiology of human listeriosis. MicrobesInfect 2007; 9: 1236–43.
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
Copyright (c) 2009 Isabela Mateus Martins, Dirce Yorika Kabuki, Arnaldo Yoshiteru Kuaye