Diversidade dos genes associados à virulência em cepas de Escherichia coli O128 isoladas em São Paulo, Brasil
PDF (English)

Palavras-chave

Escherchia coli
E.coli enteropatogênica
EPEC
Sorotipos O128

Como Citar

1.
Dias Ângela MG, Irino K, Kano E, Kato MAMF, Gomes TAT, Trabulsi LR. Diversidade dos genes associados à virulência em cepas de Escherichia coli O128 isoladas em São Paulo, Brasil. Rev Inst Adolfo Lutz [Internet]. 1º de abril de 2008 [citado 3º de maio de 2024];67(2):156-62. Disponível em: https://periodicos.saude.sp.gov.br/RIAL/article/view/32784

Resumo

Foram estudadas 103 cepas de Escherichia coli do sorogrupo O128, quanto às características fenotípicas e genotípicas associadas à virulência. Cinqüenta e nove cepas representantes de todos os sorotipos foram submetidas a ribotipagem. Os sorotipos mais freqüentes foram O128:H35 (41,7%), O128:H2 (14,6%), O128:H- (8,7%) e O128:H8 (6,8%). Diferentes grupos enteropatogênicos foram identificados. Somente as cepas do sorotipo O128:H2 foram positivas para as sondas eae and bfpA e apresentaram o padrão de adesão AL-like. Cepas do sorotipo O128:H8 e algumas imóveis reagiram apenas com a sonda eae e foram classificadas como EPEC atípicas. As cepas O128:H35 corresponderam à categoria enteroagregativa e os sorotipos O128:H7, O128:H21, O128:H27 e as cepas imóveis foram classificadas como enterotoxigênicas. Todas as cepas que apresentaram marcadores de virulência pertenciam ao grupo clonal A, enquanto que no grupo clonal B estavam incluídas as cepas desprovidas dos fatores de virulência pesquisados.
https://doi.org/10.53393/rial.2008.67.32784
PDF (English)

Referências

1. Robins-Browne, RM. Traditional enteropathogenic Escherichia coliof infantile diarrhea. Rev Infect Dis. 1987; 9:28-53.

2. Gomes, TAT, Griffin PM, Ivey C, Trabulsi LR and Ramos SRTS. EPECinfections in São Paulo. Rev Microbiol, São Paulo, 1996; 27 (Suppl.1): 25-33.

3. Franzolin MR,Alves RCB,Keller R,Gomes TAT,Beutin L,BarretoML,Milroy C,Strina A, Ribeiro H, Trabulsi LR. Prevalence of diarrheagenic Escherichia coli in children with diarrhea in Salvador, Bahia, Brazil. Mem Inst Oswaldo Cruz. 2005;100:359-63.

4. Ewing WH,Tatum HW and Davis BR. The occurrence of Escherichiacoli serotypes associated with diarrheal disease in the United States.Public Health Laboratory. 1957;15:118-38.

5. Tamura K, Sakazaki R, Murase M and Kosako Y. Serotyping andcategorisation of Escherichia coli strains isolated between 1958 and 1992from diarrhoeal disease in Asia. J. Med. Microbiol.1996;45:353-8.

6. Nguyen TV, Van PL, Huy CL, Gia KN, Weintraub A. Detection andcharacterization of diarrheagenic Escherichia coli from young childrenin Hanoi, Vietnam. J Clin Microbiol. 2005;43:755-60.

7. World Health Organization. Programme for Control of DiarrhoealDiseases. Manual for Laboratory Investigations of Acute Enteric Infections. World Health Organization,Geneva 1987.

8. Kaper, J. B. Defining EPEC.Rev. Microbiol., São Paulo 1996: 27(Suppl.1):130-3.

9. Kaper JB, Nataro JP, Mobley HLT. Pathogenic Escherichia coli .Nature Reviews Microbiol. 2004;2:123-40.

10. Taylor J, Charter RE . Escherichia coli O128 causing gastroenteritisof infants. J Clin Pathol.1955; 8:276-81.

11. Orskov F, Orskov I. Escherichia coli serotyping and disease in manand animals. Can J Microbiol.1992; 38:699-704.

12. Guth BEC, Silva MLM, Scaletsky ICA, Toledo MRF, Trabulsi LR.Enterotoxin production, presence of colonization factor antigens I ,and adherence to HeLa cells by Escherichia coli O128 strains belongingto different O subgroups. Infect Imm.1985;47:338-40.

13. Giammanco AM, Maggio G, Giammanco R, Morelli R, Minelli F,Scheutz F and Caprioli A. Characteristics of Escherichia coli strainsbelonging to enteropathogenic E.coli serogroups isolated in Italy fromchildren with diarrhea. J Clin Microbiol.1995;34:689-94.

14. Scotland SM, Willshaw GA, Smith HR, Said B, Stokes N, Rowe B. Virulence properties of Escherichia coli strains belonging to serogroups O26, O55, O111 and O128 isolated in the United Kingdom in 1991from patients with diarrhoea. Epidemiol Infect.1993;111: 429-39.

15. Dulguer MV, Fabbricotti SH, Bando SY, Moreira-Filho CA, Fagundes-Neto F, Scaletsky ICA. Atypical enteropathogenic Escherichia colistrains:phenotypic and genetic profiling reveals a strong associationbetween enteroaggregative E.coli heat-stable enterotoxin and diarrhea.J Infect Dis.2003; 188:1685-94.

16. Ewing WH. Edwards and Ewing ́s Identification of Enterobacteriaceae,4 th ed. Elsevier Publishing Co., Inc., New York 1986.

17. Maas R. An improved colony hybridization method with significantly increasedsensitivity for detection of single genes. Plasmid 1983;10: 296-8.

18. Rodrigues J, Scaletsky ICA, Campos LC, Gomes TAT, Whittam TS,Trabulsi LR. Clonal structure and virulence factors in strains of Escherichia coli of the classic serogroup O55. Infect Immun. 1996;64: 2680-6.

19. Evans Jr DJ, Evans DG. Classification of pathogenic Escherichia coliaccording to serotype and the production of virulence factor, withspecial reference to colonization factor antigens.Rev Infect Dis.1983; 5:692-701.

20. Edwards PR, Ewing WH. Identification of Enterobacteriaceae. Burgess Publishing Co., Minneapolis 1972.

21. Beutin L, Prada I, Zimmermann S, Sthephan R, Orskov I, Orskov F.Enterohemolysin, a new type of hemolysin produced by some strainsof enteropathogenic E.coli (EPEC). Zentralb. Bakteriol Hyg Reihe.1988; A 267:576-88.

22. Dean AG, Ching YC, Williams RG, Harden LB. Test for Escherichiacoli enterotoxin using infant mice; application in a study of diarrhea in children in Honollullu. J Infect Dis.1972;125:407-11.

23. Johnson WM, Lior H. A new heat labile cytolethal distending toxin(CDT) procuced by Escherichia coli isolated from clinical material. Microbiol Pathog. 1988 4:103-13.

24. Cravioto A, Gross RJ, Scotland SM, Rowe B. An adhesive factorfound in strains of Escherichia coli belonging to the traditionalinfantile enteropathogenic serotypes. Curr Microbiol.1979; 3:95-9.

25. Baldini MM, Kaper K, Levine MM, Candy DCA, Moon HW. Plasmidadhesion in enteropathogenic Escherichia coli. J Pediatr Gastroenterol Nutr. 1983; 2:534-8.

26. Bilge SS, Clausen CR, Lau W, Moseley S. Molecular characterization of afimbrial adhesin, F1845, mediating diffuse adherence ofdiarrhea-associated E.coli to HEp-2 cells. J Bacteriol. 1989;171:4281-9

27. Vial PA ,Robins-Browne R, Lior H, Prado V, Kaper JB, Nataro JP,Maneval D, Elsayed A, Levine MM. Characterization of Enteroadherent-Aggregative Escherichia coli a putative agent ofdiarrheal disease.J Infect Dis.1988;158:70-9.

28. Brenner DJ, McWhorter AC, Knutson JKL, Steigerwalt AD .Escherichia vulneris: a new species of Enterobacteriaceae associatedwith human wounds. J Clin Microbiol. 1982;15:1133-46.

29. Southern EM. Detection of specific sequence among DNA fragmentsseparated by gel electrophoresis. J Mol Biol.1975; 98:503-17.

30. Popovic T, Bopp CA, Olsvik O, Wachmuth K. Epidemiologicapplication of a standardized ribotype scheme for Vibrio cholerae O1.J Clin Microbiol. 1993;31:2474-82.

31. Dalla-Costa LM, Irino K, Rodrigues J, Rivera ING, Trabulsi LR. Characterization of diarrheagenic E.coli clones by ribotyping andERIC-PCR. J Med Microbiol.1998; 47:227-34.

32. Campos LC, Whittam TS, Gomes TAT, Andrade JRC and TrabulsiLR. Escherichia coli serogroup O111 includes several clones of diarrheagenic strains with different virulence properties. Infect Imm.1994;62:3282-8.

33. Gonçalves AG, Campos LC, Gomes TAT, Rodrigues J, Sperandio V,Whittam TS and Trabulsi LR. Virulence properties and clonal stuctureof strains of E.coli O119 serotypes. Infect Immun. 1997;65:2034-40.

34. Nguyen RN, Taylor LS. Tauschek M, Robins-Browne RM. Atypicalenteropathogenic Escherichia coli infection and prolonged diarrheain children. Emerg Infect Dis.2006;12:597-603.

35. Ewing WH, Davis BR, Montague TS. Studies on the occurrenceof Escherichia coli serotypes associated with diarrheal disease. Atlanta, GA. U.S. 1963, Department of Health, Education andWelfare.

36. Baudry B, Savarino SJJ, Vial P, Kaper JB, Levine MM. A sensitive andspecific DNA probe to identify Enteroaggregative Escherichia coli, arecently discovered diarrheal pathogen. J Infect Dis.1990; 161: 1249-51.

37. Nataro JP. Non-EPEC E.coli that adhere to HEp-2 cells. Rev Microbiol., São Paulo 1996; 27(suppl.1): 67-71.

38. Whittam TS, McGraw EA . Clonal analysis of EPEC serogroups. Rev Microbiol., São Paulo 1996;27 (Suppl.1): 7-16.

39. Whittam TS, Wolfe ML, Wachsmuth IK, Orskov F, Orskov I, Wilson RA. Clonal relationship among Escherichia coli strains that causehemorrhagic colitis and infantile diarrhea. Infect Immun. 1993 ;61:1619-29.

40. Orskov F, Whittam TS, Cravioto A, Orskov I . Clonal relationshipsamong classic enteropathogenic Escherichia coli (EPEC) belongingto different O groups. J Infect Dis.1990;162:76-81.

Creative Commons License
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.

Copyright (c) 2008 Ângela Maria Girardi Dias, Kinue Irino, Elena Kano, Maria Aidê Mitiko Fukushima Kato, Tânia Aparecida Tardelo Gomes, Luiz Rachid Trabulsi

Downloads

Não há dados estatísticos.