Use of various brands of basic fuchsins in the preparation of the Schiffs' reagent for staining of aldehydes in tissue sections
, ORIGINAL ARTICLE

Use of various brands of basic fuchsins in the preparation of the Schiffs' reagent for staining of aldehydes in tissue sections

ORIGINAL ARTICLE
https://doi.org/10.53393/rial.1975.35.37083
Published 1976-12-30
Evandro Pimenta de Campos
Instituto Adolfo Lutz
Antonio James Brandi
Instituto Adolfo Lutz
Adriana Manginelli Massignani
Instituto Adolfo Lutz
Nilza Baptista
Instituto Adolfo Lutz
pdf (Português (Brasil))

Keywords

leucofuchsin, preparation
staining, aldehyde in tissue
aldehyde, staíning ín tissue

How to Cite

1.
Campos EP de, Brandi AJ, Massignani AM, Baptista N. Use of various brands of basic fuchsins in the preparation of the Schiffs’ reagent for staining of aldehydes in tissue sections. Rev Inst Adolfo Lutz [Internet]. 1976 Dec. 30 [cited 2024 May 18];35(1-2):127-30. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/37083
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Abstract

Any brand of basic fuchsins can be used in the preparation of Schiff's reagent for stading of aldehydes rather than only those indicated in the histologic techniques literature, provided the following guidelines ave observed: a) oxygen in the water is to be eliminated to the maxímurn extent possible by boiling the water for 15 minutes; b ) addition of fuchsin to the water is to be made only after heating is discontinued; c) metabissulfite must be added at the bottom of the acidified reagent: d) no trace of coa1 is to be 1eft in the solution; d) reagent is not to have contact with aír during storage.

https://doi.org/10.53393/rial.1975.35.37083
pdf (Português (Brasil))

References

1. DAVENPORT, A.H. - Histological and h.istochemical technics. Philadelphia, Saunders [c1960j. p. 187.

2 LILLIE, R. D. - Histopathologic tecnic and practical histochemistry. 3th ed. New York, McGraw-Hill [c1965j. p. 496.

3. LISON, L. - Histochimie et cytochimie animales. Principes et méthodes. 3éme ed. Paris, Gauthier- Villars, 1960. v. 2, p. 433.

4. PEARSE, A.G.E. - Hietoqiiirnica teorica y aplicada. Versión espafiola de Tomas Palomo Salas. Madrid. Aguilar, 1960. p. 473

5 . Ibid p. 175.

6. Ibid p. 472.
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Copyright (c) 1976 Instituto Adolfo Lutz Journal

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