Keywords
hepatitis B, antibody
How to Cite
Abstract
A study of the hepati tis B surface antigen (HBsAg) prevalence was carried out in 17,401 blood-bank donors from the city of São Paulo using
radíoímmuneassay (RIA) and reverse passive hemagglutination (R-PHA) tests. The resulta were 1.270/0 and 1.130/0, respectively, with a correlation of 89.140/0 between the two techniques. Detection of hepatitis Be antigen (HBeAg) and the respective antibody (anti-HBe) was ma de in 139 HBsAg positive sera by means of agarose gel ímmunodíffuaion test (AGID). A positivity of 7.20/0 for the antigen and 280/0 for the antíbody was obtained. The presence of both markers ín the same serum specimen was not observed. Four hundred specimens were selected among the HBsAg nega tive sera to study the prevalence of the antibody against HBsAg (anti-HBs) and hepatitis B core antigen (anti-HBc) by radioimmunoassay. The resulta showed that 7.250/0 were positive for anti-HBs only, 4.50/0 for anti-HBc,
and 10.50/0showed both rnarkers. A more careful study of sera positive for anti-HBc only, would be necessary since it is still questionable whether these specimens may represent a transrnission risk for the post-transfusion Hepatitis B.
References
2. AXEN, R & ERNBACK, S. - Chemical fixation of enzymes to cyanogen halide
activated polysaccharide carriers. Eur, J. Biochem., 18 :351-360, 1971.
3. DEINHARDT, F. & GUST, LD. - Viral hepatitis. Bull WHO, 60: 661-91, 1982.
4. GOLDFIELD, M.; BLACK, H.C.; BILL, J.; SRIHONGSE, S. & PIZZUTI, W. - The
consequence of administering blood pretest for HBsAg by third geration techrii-
ques : a progress reporto Am. J. medo Sci., 270 :335-42, 1975.
5. GREENWOOD, F.C.; HUNTER, W.M. & GLOVER, J.S. - The preparation of
131I-labelled human growth hormone of high specific radioactivity. Bioch. J., 89:
114-23, 1963.
6. HOOFNAGLE, J.H. et alii - Type B hepatitis after transfusion witlr blood containing antibody to hepatitis B core antigen. New Eng. J. Med., 298:1379-83, 1978.
7. HOUVEN, B.; GOUDEAU, A. & DANKERT, J. _ Isolation of hepatitis B surface an tigen (HBsAg) by affinity chromatography on antibody coated ímmunoadsorbents. J. immunol. Methods, 8:185-94, 1975.
8. IMAI, M.; YAMASHITA, Y.; MIYAKAWA, Y. & MAYUMI, M. - Hemmagglutination inhibition assay of tlre common determinants and subspecificities of australia antigen. Immunology, 27:871-8, 1974.
9. KATCHKI, J.N.; SIEM, T.H.; BROUWER, R.; BRANDT, K.H. & VAN DER WAART,
M. - Detection and significance of anti-HBc in the blood bank. Prelimminary results of a controlled prospective study. J. viro I. M ethods, 2: 119-25, 1980.
10. KIMURA, R.T.; TACHIBANA, C.F.; CURY, V.L. & TAKEDA, A.K. - Reações imunológicas para detecção do antígeno de superfície da hepatite B (HBsAg). Rev. Inst. Adolfo Lute, 38: 83-6, 1978.
11. LANDER, J.J.; GITNICK, G.L.; GELB, L.H. & ASCH, R.D. - Anticore antibody sereening of transfused blood. Vo<x sang., 34 (2) : 77 -80, 1978.
12. MAZZUR, S.; NATH, N.; FANG, C.; BASTIANS, M.J.; MOLINARIS, J.L.; FAY, O.H.; LABRADOR GONZALES, G.; GONZALES, G.; GUTIERREZ, A.; JARAMIL-
LO, C.; KATZ, R; DE ME LOPEZ, M.B.; LEVY, E.; MORALES AYALA, F.; RODRIGUES AMAYA, J.; RODRIGUES MOYADO, H.; TORRES RA. & VELASCO, M. - Distribution of hepatitis B virus (HBV) markers in blood donors of 13 western hemisphere contries. Proceedings of the red cross latin american hepatitis B workshop. Bull, Pano Amer. Health Organ., 14:44-51, 1980.
13. OKADA, K.; KAMIYAMA, L; INOMATA, M.; IMAI, M.; MIYAKAWA, Y. & MAY-
UM I, M. - "e" antigen and an ti-e i n the serum of asymptomatic carrier mothers as indicators of positive and nega tive transmission of hepatitis B virus to their infants. Neui Engl. J. Med., 294:746-9, 1976.
14. PEREIRA, J.M. & CALLADO, A.N.A. Pesquisa do an tígeno Australia nos doa-
dores de sangue do Estado da Guanabara. Boi. Inst. Est. Hemat ol: "Artur Siqueira Cavalcanti", Rio de J., 2:5-11, 1972.
15. PURCELL, R.H. & BARKER, L.F. - Hepatitis B virus. In: ROSE, N.R & FRIEDMAN, H., ed. - Manual of clinical immunology. Washington, DC, American Society of Microbiology, 1976.
16. ROSEMBLIT, J.; TAKEI, K.; AMATO NETO, V.; MENDONÇA, J.S. & MELO, E.
Pesquisa do an tigeno de hepatite B no soro de doadores de sangue em serviço
de hemoterapia da cidade de São Paulo. Rev. bras. Clin. terap., 4: 121-2, 1975.
17. SHIKATA, R.; KARASANA, T.; ABE, K.; UZAWA, T.; SUZUKI, H.; ODA, T.; IMAI, M.; MAYUMI, M. & MORITSUGU, Y. - Hepatitis B, e antigen and infectivity of hepatitis B virus. J. infeet. Die., 136: 571-6, 1977.
18. TAKAHASHI, K.; IMAI, M.; TSUDA, F.; TAKAHASHI, T.; MIYAKAWA, Y. &
MA YUMI, M. - Association of Dane particles with "e" antigen in the serum
of asymptomatic carriers of hepatitis B surface antigen. J. Immunol., 117:102-5,
1976.
19. YOSHIDA, C.F.T.; GASPAR, A.M.C.; MARZOCH, K.B.F. & SCHATZMAYR, H.G. HBsAg, subtypes and HBsAb in the white and asiatic populations from urban and rural communities in the South of Brazil. Rev. Microbiol., São Paulo, 10: 106-9, 1979.
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