Keywords
saúde única
zoonoses
patologia
imuno-histoquímica
How to Cite
Abstract
Objective: describe wild fauna casuistry data evaluated in 2019, in a reference laboratory in
the State of São Paulo, Brazil, through histopathological and immunohistochemical analysis
after the implementation of a pilot project of wildlife disease laboratory surveillance. Methods:
descriptive study with survey of samples of non-human primates, birds and other mammals,
received from two screening centers in the city of São Paulo. For this purpose, the referral
forms and the issued histological and immunohistochemical reports were reviewed and data was
tabulated and analysed by frequency and percentage distribution. Results: 233 animals of 20
distinct genera and/or species were received, being 191 (81,9%) non-human primates, 25 birds
(10,7%) and 17 other mammals (7,3%); zoonotic pathogens were detected among the studied
population, with a prevalence of bacterial conditions among the conclusive cases. Conclusion:
histological and immunohistochemical analysis of wild fauna samples due the implementation
of a wildlife disease laboratory surveillance pilot program contributed to the detection of some
pathogens of public health relevance in Sao Paulo city, Brazil.
References
Grogan LF, Berger L, Rose K, Grillo V, Cashins SD, Skerratt LF. Surveillance
for Emerging Biodiversity Diseases of
Wildlife. PLoS Pathog. 2014;10(5). BEPA 2021;18(205):1-12 página 11
Estudo descritivo: histopatologia e imuno-histoquímica para a detecção de patógenos em amostras de fauna selvagem
recebidas pelo Instituto Adolfo Lutz, Brasil/Santos ALM et al.
Woolhouse MEJ, Gowtage-Sequeria S. Host range and emerging and
reemerging pathogens. Emerg Infect Dis. 2005;11(12):1842-7.
Nakayima J, Hayashida K, Nakao R, Ishii A, Ogawa H, Nakamura I, et
al. Detection and characterization of
zoonotic pathogens of free-ranging
non-human primates from Zambia.
Parasites and Vectors. 2014;7(1):1-7.
Paz FAZ, Bercini MA. Doenças
Emergentes e Reemergentes no Contexto
da Saúde Pública Boletim
da Saúde
Doenças Emergentes e Reemergentes
no Contexto da Saúde Pública. Esc
Saúde Pública. 2009;23(1):1-3.
Mackey TK, Liang BA, Cuomo R, Hafen
R, Brouwer KC, Lee DE. Emerging and
reemerging neglected tropical diseases: A
review of key characteristics, Risk factors,
And the policy and innovation environment.
Clin Microbiol Rev. 2014;27(4):949-79.
Cunningham AA, Daszak P, Wood JLN.
One health, emerging infectious
diseases and wildlife: Two decades
of progress? Philos Trans R Soc
B Biol Sci. 2017;372(1725).
Rabinowitz PM, Kock R, Kachani M,
Kunkel R, Thomas J, Gilbert J, et al.
Toward proof of concept of a one health
approach to disease prediction and
control. Emerg Infect Dis. 2013;19(12).
Vallat B. Improving wildlife surveillance
for its protection while protecting us
from the diseases it transmits. 2008.
Editoral on-line do sítio da OIE.
Acessado em janeiro de 2020.
Artois M, Bengis R, Delahay RJ,
Duchêne M-J, Duff JP, Ferroglio E,
et al. Wildlife Disease Surveillance
and Monitoring. In: Management of
Disease in Wild Mammals. 2009.
Brasil, Ministério da Saúde. PORTARIA
DE CONSOLIDAÇÃO No 4, DE 28
DE SETEMBRO DE 2017. 2017.
Ellwanger JH, Chies JAB. Zoonotic
spillover and emerging viral diseases – time
to intensify zoonoses surveillance in Brazil.
Brazilian J Infect Dis. 2018;22(1):76-8.
East M, Res V, Guardian T, Health TO,
Health O, Food UN, et al. Emerging
zoonoses: A one health challenge.
EClinicalMedicine. 2020;19.
Brasil, Ministério da Saúde. Secretaria
de Vigilância em Saúde. MANUAL DE
VIGILÂNCIA DE EPIZOOTIAS EM
PRIMATAS NÃO-HUMANOS. 2017.
Bidaisee S, Macpherson CNL. Zoonoses
and one health: A review of the
literature. J Parasitol Res. 2014;2014.
Catão-Dias JL. Doenças e seus
impactos sobre a biodiversidade.
Cienc Cult. 2003;55(3):32-4.
Cardoso FA. Desenvolvimento e
validação de um ensaio de PCR-ELISA
para o diagnóstico da leishmaniose
visceral humana em amostras de sangue
periférico Desenvolvimento e validação
de um ensaio de PCR-ELISA para o
diagnóstico da leishmaniose visceral
humana em amostras. Mestr (Programa
Pós-graduação em Ciências da Saúde)
– IMinistério da Saúde, Fundação
Oswaldo Cruz, Cent Pesqui René
Rachou, Belo Horiz - MG. 2013;109.
Michalany J. Técnica histológica em
anatomia patológica: com instruções para o
cirurgião, enfermeira e citotécnico. 3rd ed.
São Paulo: Editora Michalany; 1998. 295 p.
Metovic J, Bertero L, Musuraca C,
Veneziano F, Annaratone L, Mariani S,
et al. Safe transportation of formalinfixed
liquid-free pathology specimens.
Virchows Arch. 2018;473(1):105-13.
BEPA 2021;18(205):1-12
página 12
Estudo descritivo: histopatologia e imuno-histoquímica para a detecção de patógenos em amostras de fauna selvagem
recebidas pelo Instituto Adolfo Lutz, Brasil/Santos ALM et al.
Chafin D. Pre-Analytics of Pathological
Specimens in Oncology. Recent Results
Cancer Res. 2015;199:107-17.
Mcdonnell G, Russell AD. Antiseptics
and disinfectants: Activity, action,
and resistance. Clin Microbiol
Rev. 1999;12(1):147-79.
Chua J, Bozue JA, Klimko CP, Shoe JL,
Ruiz SI, Jensen CL, et al. Formaldehyde
and glutaraldehyde inactivation of
bacterial tier 1 select agents in tissues.
Emerg Infect Dis. 2019;25(5):919-26.
Nielsen GD, Larsen ST, Wolkoff P.
Recent trend in risk assessment of
formaldehyde exposures from indoor
air. Arch Toxicol. 2013;87(1):73-98.
Patel PG, Selvarajah S, Boursalie S,
How NE, Ejdelman J, Guerard KP,
et al. Preparation of formalin-fixed
paraffin-embedded tissue cores for
both RNA and DNA extraction. J
Vis Exp. 2016;2016(114):1-10.
OIE. Training manual on surveillance
and international reporting of diseases
in wild animals. Workshop for OIE
National Focal Points for Wildlife
Second Cycle. 2015;96. Disponível em:
http://www.oie.int/fileadmin/Home/
eng/Internationa_Standard_Setting/
docs/pdf/WGWildlife/A_Training_
Manual_Wildlife_2.pdf. Acessado
em dezembro de 2019.
Morner T, Obendorf DL, Artois M,
Woodford MH. Diseases of wildlife
occur in many different forms in a
wide range of animal species and
populations. Rev Sci Tech [Internet].
;21(1):67-76. Disponível em:
https://pdfs.semanticscholar.org/febb/
e72403d6cfaf0ce328b0af28bef1fe5d2685.
pdf. Acessado em janeiro de 2020.
Maas M, Gröne A, Kuiken T, Van Schaik
G, Roest HIJ, Van Der Giessen JWB.
Implementing wildlife disease surveillance
in the Netherlands, a One Health approach.
OIE Rev Sci Tech. 2016;35(3):863-74.
Linden A, Wirtgen M, Volpe S, Nahayo A,
Pirson J, Paternostre J, et al. Surveillance
of wildlife diseases in Belgium. Epidémiol
santé anim. 2011;(59-60):213-5.
Smith A, Woutrina A, Jonna AK, Dwight
C. Salmonella in California Wildlife
Species: Prevalence in Rehabilitation
Centers and Characterization of Isolates.
J Zoo Wildl Med. 2002;33(3):228-35.
Radhouani H, Silva N, Poeta P, Torres
C, Correia S, Igrejas G. Potential impact
of antimicrobial resistance in wildlife,
environment, and human health. Front
Microbiol. 2014;5(FEB):1-12.
Williams ES, Yuill T, Artois M, Fischer J,
Haigh SA. Surveillance and monitoring
of wildlife diseases. Rev sci tech
Off int Epiz. 2002;21(1):139-57.
Dettmeyer RB. The role of histopathology
in forensic practice: an overview. Forensic
Sci Med Pathol. 2014;10(3):401-12.
Tavichakorntrakool R, Prasongwattana
V, Sriboonlue P, Puapairoj A, Pongskul
J, Khuntikeo N, et al. Serial analyses of
postmortem changes in human skeletal
muscle: A case study of alterations in
proteome profile, histology, electrolyte
contents, water composition, and
enzyme activity. Proteomics Clin.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2021 Alessandra Loureiro Morales dos Santos, Filipe Onishi Nagamori, Isis Paixão de JesusI, Camila Santos da Silva Ferreira, Paloma Martins do Nascimento, Sandra Alves da Silva, Julia de Carvalho, Ticiana Martins Zwarg, Amanda Aparecida Cardoso Coimbra, Thais Sanches, Bruno Petri, Liliane Milanelo, Rosangela Santos de Araújo, Silvana Mello Pereira da Silva, Rodrigo Albergaria Ressio, Cinthya dos Santos Cirqueira, Cristina Takami Kanamura, Juliana Mariotti Guerra, Leila Del Castillo Saad, Roberta M. Fernandes Spinola, Gizelda Katz, Mariane Ingara de Moraes Costa, José Luiz Catão Dias, Natália Coelho Couto de Azevedo Fernandes