Keywords
Validation
Laboratory Diagnosis
Bacterial Meningitidis.
Neisseria meningitidis
How to Cite
Abstract
Counterimmunoelectrophoresis (CIE) is widely used in Brazil for non-culture diagnosis of meningitis caused by Neisseria meningitidis (Men) of serogroups A, B, C, W135 or Haemophilus influenzae serotype b (Hib). The objective of this study was to validate CIE using 301 samples of cerebrospinal fluid (CSF) and serum samples from 236 patients with suspected bacterial th meningitis in the cities of São Paulo and Campinas during the period comprised between June 4 , th 2007 to May 30 , 2009. In CSF specimens, CIE had a sensitivity of 62.7% (42/67) (95% CI, 50%- 74.2%) and specificity of 88.9% (208/234) (95% CI, 84.1% -92.6%) for the detection of Men. In serum, the sensitivity was 35.3% (6/17) (95% CI 14.2% -61.7%) and specificity 90.9% (199/219) (95% CI, 86.3% -94.4%). These parameters were not calculated for the Hib component, due to the unavailability of a significant number of samples with positive culture for this bacterium. Our results showed that the CIE assay although having relatively high specificity, showed low sensitivity, especially in serum samples, indicating that CIE is not recommended as the only diagnostic test for bacterial meningitis. Additionally, it reflects the need of the use of other non[1]culture tests with high sensitivity and specificity, such as real-time PCR to improve both clinical and the public health surveillance system.
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Fukasawa LO, Salgado MM, Gonçalves MG, Custódio AV, Araújo TP, Carvalhanas TRMP, et al. Limitações no uso da técnica de contraimunoeletroforese (CIE) para o diagnóstico das meningites causadas por Haemophilus influenzaetipo b. BEPA. Bol. Epidemiol. Paul. 2010;7(76):4-12. Correspondência/Correspondence to: Lucila Okuyama Fukasawa Av. Dr. Arnaldo, 355, 11° andar CEP: 01246-902 – São Paulo/SP– Brasil Tel.: 55 11 3068-2899 E-mail: lucilaof@gmail.com Recebido em: 17/08/2011 Aprovado em: 25/06/201
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2012 Lucila Okuyama Fukasawa, Maristela Marques Salgado, Eneida G. Lemes Marques, Rachel M.B.P. Fernandes, Brigina Kemp, Telma Regina Carvalhanas , Lee H. Harrison, Cláudio Tavares Sacchi, Grupo de Trabalho das Mening