Keywords
Real time PCR
Staphylococcus aureus
How to Cite
Abstract
Data from the Sistema de Informação de Agravos de Notificação (SINAN) shows that, in São Paulo, in 2010 the genus Staphylococcus was associated with 15 cases of bacterial meningitis confirmed by culture. The aim of this study was to analyze the frequency of meningitis associated with Staphylococcus aureus in São Paulo in 2010 by using real-time PCR (PCRTR) technique. We analyzed samples of cerebrospinal fluid (CSF) and serum from 1,214 patients with suspected bacterial meningitis. For the DNA extraction from the samples we used the commercial kit Nucleospin ® Blood (Macherey-Nagel). The PCR-TR reaction used the TaqMan® system, using the nuc gene, specific for S. aureus, as target. The specie S. aureus was associated with bacterial meningitis in 16 (1.3%) of the 1,214 cases analyzed. All these cases had at least one additional laboratorial test (gram stain, culture, counterimmunoelectrophoresis and/or latex agglutination test) performed and multiplex PCR-TR for Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae negative. None of these cases has been confirmed as associated with genus Staphylococcus in SINAN. The PCR-TR technique may represent an additional tool to culture for the etiologic diagnosis of meningitis associated with S. aureus, contributing to the increase in the number of bacterial meningitis cases of known etiology in São Paulo.
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Copyright (c) 2012 Priscilla Lima de Oliveira, Lucila Okuyama Fukasawa, Maristela Marques Salgado, Maria Gisele Gonçalves, Fábio Takenori Higa, Terezinha Pereira de Araújo, Bernadete Lourdes Liphaus, Cláudio Tavares Sacchi