Keywords
Diagnosis
Virus isolation
Capture of NS1
How to Cite
Abstract
The dengue virus, which belongs to the family Flaviviridae, genus Flavivirus, consists of single-stranded RNA encoding structural and non-structural proteins. It has four serotypes: DENV-1, DENV-2, DENV-3 and DENV-4. The rapid laboratory diagnosis may greatly help to control the spread of the disease. The objective of the present work was to evaluate diagnostic kits for dengue, by detecting the NS1 protein, using virus isolation as reference. We used 147 sera samples of patients suspected of DENV infection, 64 were tested for virus isolation and 83 by capture ELISA IgM. Dengue NS1 Ag Strip (Bio- Rad) achieved a sensitivity of 89%, specificity of 66%, PPV of 67% and NPV of 88%. Dengue Duo Test (Cassette) showed a sensitivity of 89%, specificity of 68%, PPV of 70% and NPV of 88%. The Platelia Dengue NS1 ELISA Ag (Bio-Rad) had a sensitivity of 95%, specificity of 47%, PPV of 59% and NPV of 92%. Dengue Early ELISA kit (PANBIO) resulted in a sensitivity of 86%, specificity of 71%, PPV of 69% and NPV of 86%. In general, the evaluated kits can be used in diagnosis, associated with other criteria such as clinicalepidemiological or other laboratorial methods.
References
Ministério da Saúde. Secretaria de Vigilância em Saúde. Departamento de Vigilância Epidemiológica. Doenças infecciosas e parasitárias: guia de bolso. 7. ed. Brasília: Ministério da Saúde; 2008.
World Health Organization. Dengue: guidelines for diagnosis, treatment, prevention and control. Geneva, 2009.
Pires Neto RJ, Lima DM, Paula SO, Lima CM, Rocco IM, Fonseca BAL. Molecular epidemiology of type 1 and 2 dengue viruses in Brazil from 1988 to 2001. Brazilian J Med Biol Res. 2005;38(6):843-52.
Deubel V, Nogueira RMR, Drouet MT, Zeller M, Reynes J, MA DQ. Direct sequencing of genomic cDNA fragment amplified by the polymerase chain reaction for molecular epidemiology of dengue 2 virus. Arch Virol. 1992;129:197-210.
Santos NOS, Romanos MTV, Wigg MD. Introdução à virologia humana. Rio de Janeiro: Guanabara Koogan, 2. ed.; 2002.
Alcon S, Talarmin A, Debruyne M, Falconar A, Deubel V, Flamand DM. Enzyme-linked immunosorbent assay specific to dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol. 2002;40:376-81.
Shu PY, Yang CF, Kao JF, Su CL, Chang SF, Lin CC, et al. Application of the dengue virus NS1 antigen rapid test for on-site detection of imported dengue cases at airports. Clin Vaccine Immunol. 2009;16:589-91.
Fletcher RH, Fletcher SW, Wagner EH. Clinical epidemiology: the essentials. Baltimore: Willians & Wilkins; 2. ed, 1988.
Guedes MLS. & Guedes J S. Bioestatística para profissionais de saúde. Brasília: Ministério da Ciência e Tecnologia/ Conselho Nacional de Desenvolvimento Científico e Tecnológico/Ao Livro Técnico S. A.;1988.
Gubler DJ, Kuno G, Sather GE, Velez M, Oliver A. Mosquito cell culture and specific monoclonal antibodies in surveillance for dengue viruses. Am J Trop Med Hyg. 1984;33:158-65
Casals J. Immunological technique for animal viruses. In: Moramorosh K, Koprowski H (eds): Methods in virology. 3. ed. New York: Academic Press; 1967, p.175-81.
Sever JL. Application of a microtechnique to viral serological investigations. J Immunol. 1962;88:320-9.
Clarke DH, Casals J. Technique for hemagglutination and hemagglutination inhibition with artropod-borne viruses. Am J Trop Med Hyg. 1958;7:561-73.
Poersch CO. Desenvolvimento e avaliação de métodos moleculares para o diagnóstico da dengue. [tese de doutorado]. Rio de Janeiro: Instituto Oswaldo Cruz, 2007.
Young PR, Hilditch PA, Bletchly C, Halloran W. An antigen capture enzyme-linked immunosorbent assay reveals high levels of the Dengue virus protein NS1 in the sera of infected patients. J Clin Microbiol. 2000;38:1053-7.
Fonseca BAL, Fonseca SNS. Dengue virus infections. Curr Opin Pediatr. 2002; 14(1):67-71.
Dussart P, Labeau B, Lagathu G, Louis P, Nunes MRT, Rodrigues SG, et al. Evaluation of an enzyme immunoassay for detection of dengue virus NS1 antigen in human serum. Clin Vaccine Immunol. 2006;13(11):1185-9.
Hang VT, Nguyet NM, Trung DT, Tricou V, Yoksan S, Dung NM, et al . Diagnostic accuracy of NS1 ELISA and lateral flow rapid tests for dengue sensitivity, specificity and relationship to viremia and antibody response. PLoS Negl Trop Dis. 2009;3(1):e360.
Dussart P, Petit L, Labeau B, Bremand L, Leduc A, Moua D, et al. Evaluation of two new commercial tests for the diagnosis of acute virus infection using NS1 antigen detection in human serum. PLoS Negl Trop Dis. 2008;2:e208.
Kuno G, Gomez I, Gubler DJ. Detecting artificial anti dengue IgM complexes using a enzyme linked immunosorbent assay. Am J Trop Med Hyg. 1987;36:153-9.
Lima MRQ, Nogueira RMR, Schatzmayr HG, Santos FB.. Comparison of three commercially available dengue NS1 antigen capture assays for acute diagnosis of dengue in Brazil. PLoS Negl Trop Dis. 2010;4(7):e738.
McBride WJH. Evaluation of dengue NS1 test kits for the diagnosis of dengue fever. Diagn Microbiol Infect Dis. 2009;64:31-6.
Ramirez AH, Moros Z, Comach G, Zambrano J, Bravo L, Pinto B, et al. Evaluation of dengue NS1 antigen detection tests with acute sera from patients infected with Dengue virus in Venezuela. Diagn Microbiol Infect Dis. 2009;65:247-53.
Tricou V, Vu TTH, Quynh NVN, Nguyen CVV, Tran TH, Farrar J, et al. Comparison of two dengue NS1 tests for sensitivity, specificity and relationship to viraemia and antibody responses. BMC Infectious Diseases. 2010;10:142.
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Copyright (c) 2011 Fernanda Gisele da Silva, Sarai Joaquim dos Santos Silva, Iray Maria Rocco, Vivian Regina Silveira, Akemi Suzuki, Gizelda Katz, Ivani Bisordi