Keywords
molecular diagnosis
How to Cite
Abstract
This study evaluated the ability of PCR to diagnose VL and distinguish L. (L.) chagasi from other Leishmania species. Samples from 114 dogs were divided into two groups: 44 symptomatic and 70 asymptomatic. They were assayed by parasitological methods (culture and microscopic examination) and PCR to L. (L.) chagasi, L. (V.) braziliensis; and in some cases, Leishmania spp. Parasitological tests and PCR-L. chagasi were concordant in 105 samples (92%). VL was confirmed in 49 dogs, while 56 had negative results. Of the 114 samples, 9 had discordant results but were further tested by PCR-Leishmania spp with positive results. VL was also confirmed in 4 dogs having negative parasitological tests and positive PCR-L. chagasi. Consequently, this PCR was positive for 100% (53/49) of dogs with parasites detected in parasitological tests. Also, PCR demonstrated high specificity detecting 61 dogs negative. Leishmania infection was negative in 56 dogs, and 5 with positive culture and PCR-Leishmania spp had CL since they were positive in PCR-L. braziliensis. This study shows the importance of including PCR in diagnosis of leishmaniases by differential diagnosis contributing to the surveillance and control of VL programs.
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Copyright (c) 2009 Vera Lucia Pereira-Chioccola