Paracoccidoides brasiliensis detection in armadillos (Dasypus novemcinctus) from a Savanna reserve at the Lauro de Souza Lima Institute (Bauru, SP)
Keywords:
Paracoccidioides brasiliensis, ParacoccidiodomicoseAbstract
The finding of naturally infected Dasypus novemcinctus armadillos by the fungus Paracoccidioides brasiliensis opened new perspectives for eco-epidemiological and evolution studies with this pathogen. In the present study the occurrence of P. brasiliensis in armadillos captured in the Cerrado Reservation area of Bauru/SP was evaluated. Four animals were captured (C1-C4) and after euthanasia lung, liver, spleen and mesenteric lymph nodes were collected for mycological and molecular evaluation. Ribosomal DNA fragments were amplified by PCR and Nested-PCR using P. brasiliensis specific primers. The fungus was isolated in three animals from lymph node (C1), liver (C4) and spleen samples (C2 and C4). In 3 tissue samples 387bp amplicons were identified. The detection of infected animals in the Cerrado Reservation area reinforces the use of these animals as sentinels for the presence of the pathogen in the environment. This is the first description of such evaluation in Bauru municipality
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Copyright (c) 2008 Virgínia Bodelão Richini-Pereira, Sandra de Moraes Gimenes Bosco, Severino da Graça Macoris, Raquel Cordeiro Theodoro, Silvia Cristina Barbosa Pedrini, Patrícia Sammarco Rosa, Eduardo Bagagli
This work is licensed under a Creative Commons Attribution 4.0 International License.
