Keywords
cell culture
murine neuroblastoma
bats
How to Cite
Abstract
Rabies laboratorial diagnosis is very important since clinical diagnosis is not precise. Fluorescent Antibody Test (FAT) is the most used test and eventhough it is highly sensible, accurate and fast, false negatives results may occur. Thus, the isolation of rabies virus in mice (VIM) of Central Nervous System (CNS) samples of suspected animals to be infected is also recommended and, nowadays, this test has been substituted in many laboratories by viral isolation in cell culture (VICC). The aim of the present study was to compare the sensibility of virus isolation in murine neuroblastoma (N2A) cell culture with VIM test and with FAT, as well as evaluate obtained results in the diagnostic routine from Pasteur Institute, regarding reduction of costs, time and work. A total of 105 CNS samples of different animal species were analyzed by FAT, VIM and VICC: 50 bats, 32 dogs, 13 foxes and 10 bovines. All bats and bovines samples presented concordant results for the three tests, while dogs and foxes samples presented concordance only in 24 samples (69%).Based on these results, since 2004 it has been instituted that all bat samples sent to Pasteur Institute Laboratory, after diagnosed by FAT, should be submitted to viral isolation in cell culture, replacing the use of mice. In the period of January 2004 to September 2007, 11.298 bat samples were analyzed. A total of 67 positive samples for IFD and/or VICC were also submitted to VIM, and 61 samples presented concordant results for the three tests, and showed that the use of N2A cells is more sensible to “street virus” isolation of bat samples in laboratorial routine, being faster and lower in costs than VIM.
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Copyright (c) 2007 Juliana Galera Castilho, Keila Iamamoto, Jonas Yoshitaka de Oliveira Lima, Karin Corrêa Scheffer, Pedro Carnieli Junior, Rafael de Novaes de Oliveira, Carla Isabel Macedo, Samira Maria Achkar, Maria Luiza Carrieri, Ivanete Kotait