Palavras-chave
Staphylococcus
queijo
Como Citar
Resumo
Neste trabalho foi realizado o isolamento de Staphylococcus spp. de queijo minas frescal, e para esta finalidade foi padronizado um protocolo multiplex PCR (M-PCR) para efetuar a detecção de genes de enterotoxinas clássicas (sea, seb, sec, sed e see) de Staphylococcus aureus, usando-se o gene femA como controle positivo para cepas de S. aureus. Foi testada a detecção direta de bactérias de amostras de queijo minas frescal e do alimento artificialmente contaminado. Cento e onze colônias (104 coagulase-positivas e sete coagulase-negativas) foram selecionadas e analisadas por M-PCR e, dentre essas, 34 colônias (30.62%) foram positivas para pelo menos um dos cinco genes de enterotoxinas analisados. Os genes que codificam as enterotoxinas sea e seb foram os mais frequentemente detectados. O estudo de isolamento de estafilococos coagulase-positivos revelaram que 40% das amostras demonstraram contagens bacterianas acima do limite considerado como aceitável pela legislação brasileira.Referências
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Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
Copyright (c) 2012 Juliana de Castro Beltrão da Costa, Elaine Ibrahim de Freitas, Anderson Almeida de Lemos, Carla de Oliveira Rosas, Valéria de Mello Medeiros, Márcia Barbosa Warnken, Neide Hiromi Tokumaru Miyazaki, Victor Augustus Marin