Isolation of Staphylococcus from minas frescal type cheese and detection of enterotoxin genes
Vol. 71 No. 2 (2012), ORIGINAL ARTICLE
Vol. 71 No. 2 (2012)

Isolation of Staphylococcus from minas frescal type cheese and detection of enterotoxin genes

ORIGINAL ARTICLE
https://doi.org/10.53393/rial.2012.v71.32422
Published 2012-02-01
Juliana de Castro Beltrão da Costa
Fundação Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Saúde, Departamento de Microbiologia, Rio de Janeiro, RJ
Elaine Ibrahim de Freitas
Fundação Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Saúde, Departamento de Microbiologia, Rio de Janeiro, RJ
Anderson Almeida de Lemos
Fundação Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Saúde, Departamento de Microbiologia, Rio de Janeiro, RJ
Carla de Oliveira Rosas
Fundação Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Saúde, Departamento de Microbiologia, Rio de Janeiro, RJ
Valéria de Mello Medeiros
Fundação Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Saúde, Departamento de Microbiologia, Rio de Janeiro, RJ
Márcia Barbosa Warnken
Fundação Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Saúde, Departamento de Microbiologia, Laboratório de Microbiologia de Produtos, Setor de Alimentos, Rio de Janeiro, RJ
Neide Hiromi Tokumaru Miyazaki
Fundação Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Saúde, Departamento de Microbiologia, Rio de Janeiro, RJ
Victor Augustus Marin
Fundação Oswaldo Cruz, Instituto Nacional de Controle de Qualidade em Saúde, Departamento de Microbiologia, Rio de Janeiro, RJ
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Keywords

PCR
Staphylococcus
cheese

How to Cite

1.
Costa J de CB da, Freitas EI de, Lemos AA de, Rosas C de O, Medeiros V de M, Warnken MB, Miyazaki NHT, Marin VA. Isolation of Staphylococcus from minas frescal type cheese and detection of enterotoxin genes. Rev Inst Adolfo Lutz [Internet]. 2012 Feb. 1 [cited 2024 Nov. 22];71(2):250-8. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/32422
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Abstract

This work aimed at isolating Staphylococcus spp. from minas frescal type cheese, and for this purpose a Multiplex PCR (M-PCR) was standardized for detecting the classical Staphylococcus aureus enterotoxin genes (sea, seb, sec, sed and see), using the femA gene as a positive control for S. aureus strains. Bacterial detection directly from the minas frescal type cheese and from artificially contaminated food was tested. One hundred eleven colonies (104 coagulase-positive and seven coagulase-negative) were selected for performing M-PCR assay. Thirty-four colonies (30.62%) were positive for at least one of the five enterotoxin genes analyzed; enterotoxins sea and seb were the most frequently detected. The study on Staphylococci coagulase-positive samples revealed that 40% of the samples showed bacterial counts above the limit established by Brazilian legislation.
https://doi.org/10.53393/rial.2012.v71.32422
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