Palavras-chave
orto-ftaldialdeído
estabilidade
CLAE
derivatização pré-coluna
Como Citar
Resumo
Este estudo avaliou a estabilidade do derivado fumonisina B1-orto-ftaldialdeído sob diferentes condições de pH, tempo e temperatura, durante a reação de derivatização e as análises cromatográficas. Na derivatização, a máxima fluorescência emitida pelo derivado foi obtida empregando-se a solução de tetraborato de sódio 0,1 M em pH 9,0, e esta não diferiu significativamente das emitidas em pH 8,0, 8,5, 9,5 e 10,0. Em 2 minutos de reação, obteve-se a máxima fluorescência, e esta não diferiu significativamente das emitidas em 4, 6 e 8 minutos de reação. A variação de 0,5 unidade no pH da solução tampão fosfato de sódio 0,1 M, empregada como fase móvel, influenciou significativamente na separação cromatográfica e na detecção do derivado fumonisina B1-orto-ftaldialdeído; em pH entre 3,3 e 3,8, obtiveram-se as maiores intensidades de fluorescência. O aumento na temperatura da coluna cromatográfica resultou na redução de intensidade da fluorescência. O derivado fumonisina B1-orto-ftaldialdeído manteve-se estável entre 20 e 27 °C. A 30 °C, houve redução significativa em 26,5% na intensidade da fluorescência emitida pelo derivado.Referências
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Copyright (c) 2012 Mateus Henrique Petrarca, Elizeu Antonio Rossi, Célia Maria de Sylos