Effect of gamma-irradiation (60Co) on aflatoxin B1 inactivation in peanut (Arachis hypogaea L.)
Vol. 64 No. 2 (2005), ORIGINAL ARTICLE
Vol. 64 No. 2 (2005)

Effect of gamma-irradiation (60Co) on aflatoxin B1 inactivation in peanut (Arachis hypogaea L.)

ORIGINAL ARTICLE
https://doi.org/10.53393/rial.2005.64.32982
Published 2005-02-10
Guilherme Prado
Fundação Ezequiel Dias, Laboratório de Micologia e Micotoxinas, Belo Horizonte,MG
Eliana P. Carvalho
Universidade Federal de Lavras, Departamento de Ciência de Alimentos, Lavras, MG
Marize S. Oliveira
Universidade Federal de Lavras, Departamento de Ciência de Alimentos, Lavras, MG
Jovita E. C. M. Gazzinelli
Universidade Federal de Lavras, Departamento de Ciência de Alimentos, Lavras, MG
Vanessa D. Moraes
Universidade Federal de Lavras, Departamento de Ciência de Alimentos, Lavras, MG
Ricardo F. Corrêa
Centro de Desenvolvimento de Tecnologia Nuclear, Laboratório de Medidas Nucleares, Belo Horizonte, MG
Valbert N. Cardoso
Universidade Federal de Minas Gerais, Faculdade de Farmácia, Laboratório de Radioisótopos, Belo Horizonte, MG
Thais V. Soares
Universidade Federal de Lavras, Departamento de Ciência de Alimentos, Lavras, MG
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Keywords

peanut
aflatoxin B1
gamma irradiation

How to Cite

1.
Prado G, Carvalho EP, Oliveira MS, Gazzinelli JECM, Moraes VD, Corrêa RF, Cardoso VN, Soares TV. Effect of gamma-irradiation (60Co) on aflatoxin B1 inactivation in peanut (Arachis hypogaea L.). Rev Inst Adolfo Lutz [Internet]. 2005 Feb. 10 [cited 2025 Apr. 2];64(2):186-92. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/32982
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Abstract

The effect of gamma-irradiation in doses ranging from 0 to 30 kGy was verified in order to inactivate aflatoxin B1 in peanuts sample. Peanut samples of Tatu Vermelho cultivar 2001/2002 and 2002/2003 crops,cultivated in São Paulo state, naturally or experimentally contaminated with aflatoxin B1 at varied contents, were investigated. Aflatoxin B1 was extracted with methanol:4% potassium chloride moisture (270 + 30, v/ v) followed by clarification with 10% cupric sulfate and partition with chloroform. Aflatoxin quantification was done by means of thin layer chromatography, measuring the samples and the standard fluorescent areas on densitometer at 366 nm. Gamma-irradiation (60Co) at doses of 1.5 and 10 kGy reduced the aflatoxin B1 contents in around 20% in samples experimentally contaminated with 55.3, 105.8, and 159.3 µg.kg-1. In naturally contaminated peanut samples containing 84.0 µg.kg-1 and 258.3 µg.kg-1 of aflatoxin B1 ,and treated with gamma-irradiation (60Co) at 5 and 10 kGy doses, no reduction of aflatoxin B1 levels was evidenced. On the other hand, samples naturally contaminated with aflatoxin B1 (86.0 and 248.0 µg.kg-1) and irradiated with 15, 20, 25 and 30 kGy doses, showed lower values of aflatoxin B1, ranging from 49 to 72%. In conclusion, no total inactivation of aflatoxin B1 occurs by means gamma-irradiation (60Co) in natural conditions, even at 30 kGy dose.
https://doi.org/10.53393/rial.2005.64.32982
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