Palavras-chave
contaminação de medicamentos
plantas medicinais
métodos microbiológicos
Petrifilm®
plaqueamento
Como Citar
Resumo
Técnicas alternativas aos métodos de plaqueamento em ágar para enumeração de bactérias e fungos têm sido desenvolvidas, buscando-se rapidez e simplicidade, mas sem comprometimento da sensibilidade e exatidão. O objetivo deste estudo foi investigar a eficácia e aplicabilidade de Petrifilm®AC para a enumeração de bactérias e de Petrifilm® YM para enumeração de bolores e leveduras em 90 amostras de drogas vegetais, comparando-se as contagens obtidas com a utilização dos sistemas Petrifilm® com àquelas obtidas com as técnicas de semeadura em profundidade em meios de cultura. Os coeficientes de correlação obtidos foram 0,9833 e 0,9231 para as contagens de bactérias e fungos, respectivamente. As contagens obtidas não mostraram diferenças significativas entre os métodos empregados. Os resultados sugerem a aplicabilidade do sistema Petrifilm® como alternativa ao método convencional de plaqueamento.Referências
1. Downes FP, Ito K, editors. Compendium of Methods for theMicrobiological Examination of Foods. 4th ed. Washington: APHA;2001.
2. Farmacopéia Brasileira, 4th ed. São Paulo: Ed Atheneu; 1988.
3. Beuchat LR, Copeland F, Curiale MS, Danisavich T, Gangar V, King BWet al. Comparison of the SimPlate total plate count method with Petrifilm,Redigel, and conventional Pour-Plate methods for enumerating aerobicmicroorganisms in foods. J Food Prot 1998; 61: 14-8.
4. Blackburn CW, Baylis CL, Pettit SB. Evaluation of Petrifilm methodsfor enumeration of aerobic flora and coliforms in wide range of foods.Lett Appl Microbiol 1996; 22: 137-40.
5. Chain VS, FUNG DYC. Comparison of Redigel, Petrifilm, Spiral PlateSystem, Isogrid and Aerobic Plate Count for determining the numberof aerobic in selected foods. J Food Prot 1991; 54: 208-11.
6. Cormier A, Chiasson S, Leger A. Comparison of maceration andenumeration procedures for aerobic count in selected seafoods byStandard Method, PetrifilmTM, RedigelTM and Isogrid. J Food Prot 1993;56: 249-51.
7. Ellis P, Meldrum R. Comparison of the Compact Dry TC and 3MPetrifilm ACP Dry Sheet Media Methods with the Spiral Plate Methodfor the examination of randomly selected foods for obtaining aerobiccolony counts. J Food Prot 2002; 65: 423-5.
8. Ginn RE, Packard VS, Fox TL. Enumeration of total bacteria andcoliforms in milk by dry rehydratable film methods: collaborativestudy. J Assoc Off Anal Chem 1986; 69: 527-31.
9. Mizuochi S, Kodaka H. Evaluation of dry sheet medium culture plate(Compactdry TC) method for determining numbers of bacteria infood samples.J Food Prot 2000; 63: 665-7.
10. Park YH, Seo KS, Ahn JS, Yoo HS, Kim SP. Evaluation of Petrifilmplate method for the enumeration of aerobic microrganisms andcoliforms in retailed meat samples. J Food Prot 2001; 64: 1841-3.
11. Senyk GF, Kozlowski SM, Noar PS, Shipe WF, Bandler DK. Comparisonof dry culture medium and conventional plating techniques forenumeration of bacteria in pasteurized fluid milk. J Dairy Sci, 1987;70: 1152-8.
12. Hayes MC, Ralyea RD, Murphy SC, Carey NR, Scarlett JM, Boor KJ.Identification and characterization of elevated microbial counts inbulk tank raw milk. J Dairy Sci 2001; 84: 292-8.
13. Pattison TL, Geornaras I, Holy AV. Microbial populations associatedwith commercially produced South African sorghum beer as determinedby conventional and Petrifilm plating. Int J Food Microbiol 1998; 43:115-22.
14. Beuchat LR, Nail BV, Brackett RE, Fox TL. Evaluation of a culturefilm (PetrifilmTM YM) method for enumeration of yeasts and molds inselective dairy and high-acid foods. J Food Prot 1990; 53: 869-74.
15. Beuchat LR, Nail BV, Brackett RE, Fox TL. Comparison of Petrifilm TMyeast and mold culture film method to conventional methods forenumerating yeasts and molds in foods. J Food Prot 1991; 54: 443-7.
16. Bovill R, Bew J, Robinson S. Comparison of selective media for therecovery and enumeration of probiotic yeasts from animal feed. Int JFood Microbiol 2001; 67: 55-61.
17. Spangenberg DS, Ingham SC. Comparison of methods for enumerationof yeasts and molds in shredded low-moisture, part-skim mozzarellacheese. J Food Prot 2000; 63: 529-33.
18. Taniwaki MH, Iamanaka BT, Banhe AA. Comparison of culture mediato recover fungi from flour and tropical fruit pulps. J Food Mycol1999; 2: 291-302.
19. Taniwaki MH, Silva N, Banhe, AA, Iamanaka, BT. Comparison ofculture media, Simplate, and Petrifilm for enumeration of yeasts andmolds in food. J Food Prot 2001; 64: 1592-6.
20. Vlaemynck GM. Comparison of Petrifilm TM and plate count methodsfor enumerating molds and yeasts in cheese and yogurt. J Food Prot1994; 57: 913-4.
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
Copyright (c) 2005 Adriana Bugno, Adriana Aparecida Buzzo Almódovar, Tatiana C. Pereira, Terezinha de Jesus A. Pinto