Como Citar
Resumo
Compararam-se as reações de imunofluorescência indireta e de SABIN-FELDMAN para a toxoplasmose, em 1000 soros humanos. Encontrou-se estreita concordância quanto a reatividade ou não reatividade dos soros. Quanto a títulos, em 97,7% dos 775 soros reagentes verificou-se concordância ou diferenças de somente uma diluição. Nos demais 2,3% de soros reagentes, estas diferenças não excederam de duas diluições. Em raros soros, entretanto, observaram-se divergências entre os resultados, negativos na reação de imunofluorescência e reagentes na de SABIN-FEDLMAN. Porém tal reatividade apresentou-se instável, tendendo a desaparecer rapidamente desses soros, embora mantidos congelados a -20°C, em contraste com a estabilidade de títulos observada habitualmente nos soros reagentes. Sugere-se a presença de fator modificador de citoplasma, de natureza inespecífica, para os soros de reatividade instável, diverso do fator específico presente no comum dos soros reagentes.
Referências
1. BOLDECH, V. & JIRA, J. - Bemerkungen zur Methodik und Auswertung der Komplement bindungsreaktion mit den Toxoplasmaantigen. Z. Tropenmed. Parasito 12:385-409, 1961.
2. CAMARGO, M. E. - Improved technique of indiret immunofluorescence for serological diagnosis of toxoplasmosis. Rev. Inst. Med. Trop. S. Paulo 6:117-118, 1964
3. CAMARGO, M. E. -' Preparation of microscopical slides to simplify immunofluorescence serological titrations. Rev. Inst. Med. Trop. S. Paulo 7:39-40, 1965.
4. CATHIE, J. A. B. - An appraisal of the diagnostic value of the serological tests for toxoplasmosis. Trans. Roy. Soe. Trop. Med. Hyg. 51 :104-110, 1957.
5. CHADWICK, C. S.; Mc ENTEGART, M. G. & NAIRN, R. C. - Fluorescent protein tracers: a simples alterna tive to fluorescein. Lancet 1 :412-414, 1958.
6. CLARK, H. F. & SHEPARD, C. C. - A dialysts technique for preparing fluorescent antibody. Virology 20: 642-644, 1963.
7. COONS, A. H.; CREECH, H. J. & JONES, R. N. - Immunological properties of an antibody containing a fluorescent group. Proc. Soc. Exp. Biol. Med. 47: 200-202, 1941
8. COONS, A. H. & KAPLAN, M. H. - Localization of antigens in tissue cells. 11. Improvements In a method for the detection of antigen by means of fluorescent antibody. J. Exp. Med. 91 :1-13, 1950.
9. CURTAIN, C. C. - Electrophoresis of fluorescent antibody. Nature, 182 :1305-1306, 1958.
10. DALLENBACH, F. & PIEKARSKI, G. - über der Antiki:irper (Methode nach COONC). VirGewebe mit Hilfe markierter fluorescierender Antiki:irper (Methode nach COONS). Virchows Arch. Path. Anat. 333:607-618, 1960.
11. DESMONTS, G. - Diagnostic sérologique de Ia toxoplasmose. Path. et Biol. 8:109-125, 1960.
12. EMMART, E. VI. - Observations on the absorption spectra of fluorescein, fluorescein derivaties and conjugates. Arch. Biochem. 73:1-8, 1958.
13. FROMMHAGEN, L. H. & SPENDLOVE, R. S. - The staining properties of human serum proteins conjugated with purified fluorescein ísothíocyanats. J. Immun. 89:124-131, 1962.
14. FULTON, J. D. & VOLLER, A. - Evaluation of immunofluorescent and direct agglutination methods for detection of specific Toxoplasma antibodies. Brit. Med. J. 2:1173-1175, 1964.
15. GARIN, J. P. & AMBROISE-THOMAS, P. '_ Le diagnostic sérologique de Ia toxoplasmose par Ia méthode des anticorps fluorescents (technique indirecte). Presse Méd. 71:2485-2488, 1963.
16. GOLDMAN, M. - Observations on some problems encountered in the routine performance of the dye test for toxoplasmosis. J. Clin. Path. 9 :55-58, 1956.
17. GOLDMAN, M. - Staining Toxoplasma gondii with fluorescein labelled an tíbody. I. The reaction in smears of peritoneal exsudate. J. Exp. Med. 105:549-556. 1957.
18. GOLDMAN, M. - Staining Toxoplasma gondii with fluorescein tlabelled antibody. 1'. The new serologic test for antibodies to Toxoplasma based upon inhibition of specific staining. J. Exp. Med. 105:557-573, 1957.
19. GOLDMAN, M. & CARVER, R. K. -- Microfluorimetry of cells stained with fluorescent antibody. Exp, Cell. Res. 23 :265-280, 1961.
20. GOLDMAN, M.; GORDON, M. A. & CARVER, R. K. - Comparison of titers of dye and fluorescence-inhibition tests in the serologic diagnosis of toxoplasmosis. Amer. J. Clin. Path. 37:::>41-550, 1962.
21. GOLDSTEIN, G.; SLIZYS, J. & CHASE, !VI. W. - Studies on fluorescent antibody staining. r. Non-specific fluorescence with fluorescein coupled sheep antirabbit globulins. J. Exp. Med. 114:89-110, 1961.
22. GOLDWASSER, R. A. & SHEPARD, C. C. - Staining complement and modifications of fluorescent antibody procedures. J. Immunol. 80: 122-131, 1958.
23. GORNALL, A. G.; BARDAWILL, C. J. & DAVID, M. M. - Determination of serum proteins by means of the biuret reaction J. Biol. Chem. 177:751-766, 1949.
24. GRbNROOS, P. - The action of properdin on Toxoplasma gondii. Ann. Med. Exp. Fenn. 33: 310-315, 1955.
25. HULDT, G. - The dye test and complement fixation test in toxoplasmosis: a comparative investigation. Acta path. microbiol. Scand. 43:141-156, 1958.
26. JACOBS, L. & COOKS, K. - Variations in the dye test for toxoplasmosis. Amer. J. Trop. Med. 3:860-867, 1954.
27. JACOB3, L. & LUNDE, M. N. - A hemagglutination tcst for toxoplasmosis. J. Par así t. 43: 308-314, 1957.
28. JETTMAR, H. M. - Zum Nachweis toxoplasmafeindlicher Qualitaten im Menschenserum. Wien Klin. Wschr. 66 :276 1954.
29. KABAT, E. A. & MEYER, M. M. - Experimental Immunochemistry. 22. Estimation of protein wlth the Folin-Ciocalteau phenol reagent. Springfield, Charles C. Thomas, c 1961. p. 556-558.
30. KABELITZ, H. J. - Díe díagnostísche Bedeutung und Klinische Beurteilung der Toxoplasma-Seroreaktionen und des Toxoplasmin-Hauttest. Z. Tropenmed. Parasito 11: 287-298, 1960.
31. KABELITZ, H, J. - Untersuchungen über unespeztfíche Mitreaktionen und Titerschwankungen ín den Toxoplasma-Seroreaktionen. Klin. wschr, 38:602-606, 1960.
32. KELEN, A. E.; AYLLON-CLEINDL, L. & LABZOFFSKY, N. A. - Indirect fluorescent antibody method in serodiagnosis of toxoplasrnosls, Canad. J. Microbiol. 8:545-554, 1962.
33. KNIERIM, F.; NIEDMAN, G. & THIERMANN, E. - La réacción de hemaglutinación aplicada aI diagnóstico serológico de Ia toxoplasmosts, Bol. Chil. Parasito 15:48-50, 1960.
34. KRAMARZH, L A. - Use of the fluorescent antibody method in the serologicaI díagnosís of toxoplasmosis. Med. Parazit. (Mosk). 32: 454-460, 1963.
35. LACAZ, C. S. - Contribuição para o estudo dos anticorpos bloqueadores através da prova de Coombs, Mourant e Race. São Paulo, 1953. Tese Prof. F.M.U.S.P.
36. LE'WIS, V. J. et alíí - Technical considerations in the preparation of fluorescent antibody conjugates. Appl. Microbiol. 12:343-348, 1964.
37. LEWIS, W. P. & KESSEL, J. F. - Hemagglutination in the diagnosis of toxopIasmosis. Arch. Ophthal. 66:471-476, 1961.
38. LUNDE, M. N.; JACOBS, L. & WOOD, R. M. - Comparison or dye and hemagglutination tests on sera of suspected cases of toxopIasmic uveitis. Arch. Opht.haI. 6.9:10-12, 1962.
39. SPILLANE, J. T. & Factors affecting the McKINNEY, R. M.; PEARCE, G. W. rate of reaction of fluoreseein ísothlocyana te with serum proteins. J. Immunal. 93: 232-242, 1964.
40. MALONEY, E. D. & KAUFMAN, H. E. - The rapid and convenient deteetion of Toxoplasma antibodies usíng formaIdehyde-treated human erythrocytes. Amer. J. Ophthal. 50: 945-950, 1960.
41. MANDRAS, A.; VANINI, G. C. & CIARLINI, E. - La reazione di immunofluorescenza per Ia dimostrazione degli antícorpo contro "Toxoplasma qoruiii", Ig. Mod. 56:636-644, 1962.
42. MARSHALL, J.D.; EVELAND, W.C. &SMITH, C.W. - Superlortty of fluorescein ísothíocyanate (Riggs) for fluorescent antibody technic wlth a modification of íts appüca tíon. Proc. Soc. Exp. nroi. Med. 98:898-900, 1958.
43. MAYERSBACH, H. & SCHUBERT, G. - Die unespezifisehen Reaktionen zwíschen markierten Seren und Geweben beí der ímrnunohistologischen Technik. Acta Histochem. 10: 44-82, 1960.
44. MEIRA, J. A. et alii - Resultados de reações sorológicas para o diagnóstico da Toxoplasmose efetuados com soros de pacientes com protozooses. Hospital 55:691-698, 1959.
45. MITCHELL, R. G. & GREEN, C. A. - The hemagglutination test for Toxoplasma antíborües. J. Clin. Path. 13:331-335, 1960.
46. NI'CHOLS, R. L. & Me COMB, D. E. Immunofluorescent studies wíth traehoma and related antígens. J. Immun. 89:545-554, 1962.
47. PORTNOY, L & GARSON, W. - New and ímproved antigen suspension for rapíd reactíon ín test for syphílís. Pub. Hlth , Rep. 75: 985-988, 1960.
48. REUSS, K. - Untersuchungen zur Hârna.ggtutinationsreaktion aur ToxopIasmose nach Jacobs und Lunde. Z. Immun. Forsch. 121: 75-90, 1961.
49. RIGGS, J. L. et alii - Isothiocyanate compounds as fluorescent labeling agents for immune serum. Amer. J. Path. 34:1081-1097, 1958.
50. RUBIN, M. - Studies on Toxoplasmosis. Amer, J. Trop. Med. 7:358-364, 1958.
51. RUCKERBAUER, G. M. et alii - Toxoplasmosis. I. Studies by the fluorescein-Iabelled antibody techinique. Canad. J. Cornp. Med. 27:27-33, 1963.
52. SABIN, A. B. & FELDMAN, li. A. - Dyes as microehemical indieators of a new immunity phenomenon affecting a protozoon parasite (Toxoplasma). Science 108:660,663, 1948.
53. STADTSBAEDER, S.; TELLIER - VERHEYDEN, N. & WEBER, E. M. - Diagnostic serologique de Ia toxoplasmose par l'immunifluorescense. Acta Clin. Belg. 19:161-166, 1964.
54. THALHAMMER, O. - Apud BOZDECH & JIRA, 1: 1961.
55. THIERMANN, E.; KNIERIM, F. & NIEDMANN, G. - Vet-g leíchende Untersuchungen über dies Sabin-Feldmanreaktion und den Haemagglutinationstest bei Infektionen mit Toxoplasma gondU. Z. Bakt. Parasíkdc. 192:230-260, 1964.
56. VAN SOESTBERGEN, A. A. 1956 - Apud VAN THIEL, 1958.
57. VAN SOESTBERGEN, A. A. - The tttratíon or toxoplasma antibody. J. Hyg. (Lond.) 60: 333-339, 1962.
58. VAN THIEL, P. H. - Das Problem der Spezifizitat des Sabin-Feldman Farbtestes. Antonie v. Leeuwenhoek. 24:113-133, 1958.
59. WELLER, T. H. & COONS, A. H. - Fluorescent an tíbody studies with agents of varicella and herpes zoster propaga ted in vitro. Proc. Soco Exp. Blol. (N.Y.). 86:789-794, 1954.
60. WESTPHAL, A. & MüHLPFORDT, H. - Untersuchungen über wesen und fehlerquellen des Toxoplasmose-Serofarbtestes nach Sabin und Feldman. Z. Hyg, Infekt. Kr. 131:423-439, 1950.
61. WITTMER, R.; ROTH, W. & FAORO, N. - Die Toxoplasmose-Haemagglutination. Ophthamologica (Basel) 141:402-409, 1961.
62. ZARDI, O. - Gli anticorpi fluorescenti nella diagnostica per Ia Toxoplasmosi. Nuovi Ann. Ig. 14:585-612, 1963.
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Copyright (c) 1964 Mário E Camargo