Palavras-chave
culturas celulares
materiais biocompatíveis
testes de toxicidade
materiais biocompatíveis
testes de toxicidade
Como Citar
1.
Cruz Áurea S, Cuppoloni KM, Martinez CH, Gomes LFS. Culturas celulares na detecção da toxicidade de materiais médico-hospitalares e outros que entram em contato com o ser humano. Rev Inst Adolfo Lutz [Internet]. 30º de dezembro de 1987 [citado 5º de maio de 2024];47(1-2):51-7. Disponível em: https://periodicos.saude.sp.gov.br/RIAL/article/view/36892
Resumo
Em testes de citotoxicidade in vitro, pelo método de difusão em ágar, para materiais de uso médico hospitalar e outros, foram usadas seis linhagens celulares estabelecidas: AV3' Vero, RC-IAL, NCTC clone 929, RV-IAL, HeLa e cultura primária de embrião de galinha (EG). Nos 20 materiais testados, as celulas EG demonstraram maior sensibilidade, seguidas das células RC-IAL e HeLa. O efeito citotóxico foi avaliado pela presença e pelas medidas dos diâmetro dos halos difundidos. Devido às dificuldades operacionais com a cultura primária EG recomenda-se, para os testes de citotoxicidade in vitro dos materiais citados, o uso de placas, em duplicata, das células RC-IAL e HeLa.
Referências
I. AMERICAN TYPE CULTURE COLLECTION - Catalogue of celllines & hybrydomas. 5th ed. Rockville, Md., ATCC, 1985. 305 p.
2. BALLS, M. & CLOTHIER, R. - Differentiated cell and organ culture in toxicity testing. Acta pharmacol. toxicol., 52(2):115-37, 1983.
3. BANDO, B.M. & ROSEMBAUM, M.J. - Toxicity in HeLa cells mediated by plastic wraps. Lab. Pract., 22:26-31,1973.
4. CHABBERT, Y.A. & VIAL, H. - Étude des substances toxiques pour les cultures de tissus en couche monocellulaire par une méthode de diffusion en gélose. Exp. Cell Res., 22:264-274, 1961.
5. CRUZ, A.S.; SAKUMA, M.E. & MARTINEZ, C.H. - RV-IAL. Nova linhagem celular de rim de vitelo. Características e susceptibilidade a alguns vírus. Rev. Inst. Adolfo Lutz, 44(1): 55-59, 1984.
6. EKW ALL, B. - Screening of toxic compounds in tissue culture. Toxicology, 17: 127-142, 1980.
7. GILL, M. - Direct cell contact screening for materiais and devices. Med. Device Diagn, Ind., 4(3):72-6, 1982.
8. GUESS, W.L.; ROSENBLUTH, S.A.; SCHMIDT, B. & AUTIAN, J. - Agar diffusion méthod for toxicity screening of plastics on cultured cell monolayers. J. Pharm. Sci., 54: 1545-7, 1965.
9. LENNETTE, E.H. & SCHMIDT, N.J., ed. - Diagnostic procedures for viral, rickettsial and chlamydial infections, 5th ed. Washington, D.C., APHA, 1979. 1138p.
10. NARDONE, R.M. - The LDso test and "in vitro" toxicology strategies. Acta Pharmacol. Toxicol., 52(2):65-79, 1983.
11. NARDONE, R.M. - The interface of toxicology and tissue culture and reflections on the carnation test. Toxicology, 17:105-11, 1980.
12. PINTO, T.l.A. - Controle de qualidade de produtos médico-hospitalares. I - Característica de biocompatibilidade de materiais polimérico. Rev. Farm. Bioquim. Univ. S. Paulo, 21(1): 41-61, 1985.
13. PINTO, T.J.A. & SAlTO, T. - Controle de qualidade de produtos médico-hospitalares: aspecto legal. Rev. Farm. Bioquim. Univ. S. Paulo, 21(2): 188-98, 1985.
14. ROSENBLUTH, S.A.; WEDDINGTON, G.R.; GUESS, W.L. & AUTIAN, J. - Tissue culture method for screening toxicity on plastic materiais to be used in medical practics. J. Pharm. Sci., 54:156-9, 1965.
15. STAREK, M. & JANDEJSEK, J. - Standar-dization of test for toxicity for some materiais used in tissue cultures laboratory. Progr. Immunobiol. Stand., 4:450-2, 1970.
16. STAMMATI, A.P.; SILANO, V. & ZUCCO, F. - Toxicology investigations with cell culture systems. Toxicology, 20:91-153, 1981.
17. TAKAHASHI, N. - Citotoxicity of mercurial preservatives in cell culture. Ophthalmic Res., 14:63-69, 1982.
18. VASINGTON, P.J.; PIERSMA, H.D.; CORBÉTT, J.J. & BITTLE, J.L. - Cytotoxicity of rubber closures in tissue culture systems. J. Pharm. Sci., 56:1276-79,1967.
19. WALUM, E. & PETERSON, A. - Acute toxicity testing in culture of mouse neuroblastoma cells. Acta Pharmacol. Toxicol., 52(2): 100-14, 1983.
20. WENZEL, D.G. & COSMA, G.N. - A quantitative metabolic inhibition test for screening toxic compunds with culture cells. Toxicology, 29:173-82, 1983.
21. WILLIAMS, G.M.; DUNKEL, V.e. & RAY, V.A. - Cellular systems for toxicity testing. Ann. N. Y. Acad. Sei., 407:1-484, 1983.
22. ZUCCO, F. - Introduction to the workshop on the application of tissue culture in toxicology. Toxicology; 17:101-4, 1980.
2. BALLS, M. & CLOTHIER, R. - Differentiated cell and organ culture in toxicity testing. Acta pharmacol. toxicol., 52(2):115-37, 1983.
3. BANDO, B.M. & ROSEMBAUM, M.J. - Toxicity in HeLa cells mediated by plastic wraps. Lab. Pract., 22:26-31,1973.
4. CHABBERT, Y.A. & VIAL, H. - Étude des substances toxiques pour les cultures de tissus en couche monocellulaire par une méthode de diffusion en gélose. Exp. Cell Res., 22:264-274, 1961.
5. CRUZ, A.S.; SAKUMA, M.E. & MARTINEZ, C.H. - RV-IAL. Nova linhagem celular de rim de vitelo. Características e susceptibilidade a alguns vírus. Rev. Inst. Adolfo Lutz, 44(1): 55-59, 1984.
6. EKW ALL, B. - Screening of toxic compounds in tissue culture. Toxicology, 17: 127-142, 1980.
7. GILL, M. - Direct cell contact screening for materiais and devices. Med. Device Diagn, Ind., 4(3):72-6, 1982.
8. GUESS, W.L.; ROSENBLUTH, S.A.; SCHMIDT, B. & AUTIAN, J. - Agar diffusion méthod for toxicity screening of plastics on cultured cell monolayers. J. Pharm. Sci., 54: 1545-7, 1965.
9. LENNETTE, E.H. & SCHMIDT, N.J., ed. - Diagnostic procedures for viral, rickettsial and chlamydial infections, 5th ed. Washington, D.C., APHA, 1979. 1138p.
10. NARDONE, R.M. - The LDso test and "in vitro" toxicology strategies. Acta Pharmacol. Toxicol., 52(2):65-79, 1983.
11. NARDONE, R.M. - The interface of toxicology and tissue culture and reflections on the carnation test. Toxicology, 17:105-11, 1980.
12. PINTO, T.l.A. - Controle de qualidade de produtos médico-hospitalares. I - Característica de biocompatibilidade de materiais polimérico. Rev. Farm. Bioquim. Univ. S. Paulo, 21(1): 41-61, 1985.
13. PINTO, T.J.A. & SAlTO, T. - Controle de qualidade de produtos médico-hospitalares: aspecto legal. Rev. Farm. Bioquim. Univ. S. Paulo, 21(2): 188-98, 1985.
14. ROSENBLUTH, S.A.; WEDDINGTON, G.R.; GUESS, W.L. & AUTIAN, J. - Tissue culture method for screening toxicity on plastic materiais to be used in medical practics. J. Pharm. Sci., 54:156-9, 1965.
15. STAREK, M. & JANDEJSEK, J. - Standar-dization of test for toxicity for some materiais used in tissue cultures laboratory. Progr. Immunobiol. Stand., 4:450-2, 1970.
16. STAMMATI, A.P.; SILANO, V. & ZUCCO, F. - Toxicology investigations with cell culture systems. Toxicology, 20:91-153, 1981.
17. TAKAHASHI, N. - Citotoxicity of mercurial preservatives in cell culture. Ophthalmic Res., 14:63-69, 1982.
18. VASINGTON, P.J.; PIERSMA, H.D.; CORBÉTT, J.J. & BITTLE, J.L. - Cytotoxicity of rubber closures in tissue culture systems. J. Pharm. Sci., 56:1276-79,1967.
19. WALUM, E. & PETERSON, A. - Acute toxicity testing in culture of mouse neuroblastoma cells. Acta Pharmacol. Toxicol., 52(2): 100-14, 1983.
20. WENZEL, D.G. & COSMA, G.N. - A quantitative metabolic inhibition test for screening toxic compunds with culture cells. Toxicology, 29:173-82, 1983.
21. WILLIAMS, G.M.; DUNKEL, V.e. & RAY, V.A. - Cellular systems for toxicity testing. Ann. N. Y. Acad. Sei., 407:1-484, 1983.
22. ZUCCO, F. - Introduction to the workshop on the application of tissue culture in toxicology. Toxicology; 17:101-4, 1980.
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
Copyright (c) 1987 Áurea Silveira Cruz, Kátia Moreira Cuppoloni, Clélia H.O. Martinez, Luís F. Salles Gomes
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