Palavras-chave
creatinina no soro humano, dosagem
Reação de Jaffé
método cinético
método de ponto-final
interferentes
Reação de Jaffé
método cinético
método de ponto-final
interferentes
Como Citar
1.
Castilho LN, Yuki MM, Oda MYN, Martins HP. Dosagem de creatinina: fatores de erro na reação de Jaffé. Rev Inst Adolfo Lutz [Internet]. 30º de dezembro de 1988 [citado 7º de novembro de 2024];48(1-2):93-8. Disponível em: https://periodicos.saude.sp.gov.br/RIAL/article/view/36919
Resumo
Estudamos o mecanismo da reação de Jaffé para a determinação da creatinina humana, utilizando os métodos cinético e ponto-final. Foi analisada a magnitude de interferência nas constantes físicas (tempo e temperatura de incubação) e no equilíbrio da reação, obtendo-se valores subestimados a 25°C nos 2 métodos estudados. Os resultados a 30°C são coerentes com os valores de referência descritos na literatura. A hiperglicemia interfere mais significantemente na reação de ponto-final, elevando os resultados de creatinina a partir de 290 mg%. O método cinético mostrou-se bastante eficaz na dosagem da creatinina sérica discriminando valores sem interferência de substâncias redutoras e com reprodutibilidade compatível às melhores técnicas laboratoriais.
Referências
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2. BUTLER, A.R. The Jaffe reaction: identification of the coloured species. Clin. Chim. ACla, 59:227-232, 1975.
3. GREENWALD, 1. & GROSS, J. The chemistry of Jaffe's reaction for creatinine, a red tautomer of creatinine picrate. J. Biol. Chem., 59:601-612, 1924.
4. HEINÉGARD, D. & T1DERSTROM. G. Determination of serum creatinine by a direct colorimetric method. Clin. Chim. ACla, 43:305-310, 1973.
5. HENRY, R.J.; eANNON, D.e. & WINKELMAN, J.W. Clinical Chemistry - Principies and Techniques. 2nd ed. Maryland, Harpes & Row, 1974. p.550.
6. KIRKPATRICK, M.; MOODY, c.. BATES, D.A. & SHAFFAR, M. Faster creatinine assay in the Abbott Spectrum System. Cl in . Chem., 33(8):1466-1467, 1987.
7. KNAPP, M.L. & MAYNE, P.D. Development ofan automated kinetic Jaffé method designed to minimise bilirribin interference in plasma creatinine
assays. Clin. Chim. ACla, 168:239-246, 1987.
8. KOSTlR, J.V. & SONKA, J. Creatinine estimation in blood serum: new method. Biochim, Biophys. Acla, 8:86-89, 1952.
9. KROLL, M.H.; ROACH, N.A.; POE, B. & ELIN, R.J. Mechanism of interference with the Jaffé reaction for creatinine. Clin. Chem., 33(7):11291132, 1987.
10. LARSEN, K. Creatinine assay by a reaction-kinetic principie. Clin. Chim. Aeta, 41:209-217, 1972.
11. LUSTGARTEN, l.A. & WENK, R.E. Simple, rapid, kinetic method for serum creatinine measurement. Clin. Chem., 18:1419-1422, 1972.
12. MARTlNEZ, E. & DOOLAN, P .D. Determination of creatinine in small quantities of plasma. Observations on two methods. Clin. Chem., 6:233-242, 1960.
13. MCLEAN, M.H.; GALLWAS, r. & HENDRIXSON, M. Evaluation of an automated creatinase creatinine procedure. Clin. Chem., 19:623-625,
1973.
14. MITCHELL, R.l. Improved method for specific determination of creatinine in serum and urine. Clin. Chem., 19:408-410, 1973.
15. PATEL, C.P. & GEORGE, R.C. Liquid chromatographic determination of creatinine in serum and urine. Ana/. Chem., 53:734-735,1981.
16. RAPOPORT,A. & MUSDAN, H. Endogenous creatinine clearance and serum creatinine in the clinícal assessment of kidney function. Cano medo Assoc. i., 99:149-156,1968.
17. TAUSSKY, H. A procedure increasing the specificity of the Jaffé reaction for determination of creatinine in urine and plasma. Clin. Chim. Aeta, 1:210-224, 1956.
18. VASILIADES, J. Reaction of alkaline sodium picrate with creatinine: L Kinetics and mechanism of formation of the mono-creatinine picric acid
complexo Clin. Chem., 22(10):1664-1671, 1976.
19. WATKINS, RE; FELDKAMP, C.S.; THIBERT, R.J. & ZAK, B. Interesting interferences in a direct serum creatinine reaction. Mieroehem, l., 21:370-384, 1976.
20. YATZIDIS, H. New method for direct determination of "true" creatmme. Clin. Chem., 20(9):1131-1134,1974.
2. BUTLER, A.R. The Jaffe reaction: identification of the coloured species. Clin. Chim. ACla, 59:227-232, 1975.
3. GREENWALD, 1. & GROSS, J. The chemistry of Jaffe's reaction for creatinine, a red tautomer of creatinine picrate. J. Biol. Chem., 59:601-612, 1924.
4. HEINÉGARD, D. & T1DERSTROM. G. Determination of serum creatinine by a direct colorimetric method. Clin. Chim. ACla, 43:305-310, 1973.
5. HENRY, R.J.; eANNON, D.e. & WINKELMAN, J.W. Clinical Chemistry - Principies and Techniques. 2nd ed. Maryland, Harpes & Row, 1974. p.550.
6. KIRKPATRICK, M.; MOODY, c.. BATES, D.A. & SHAFFAR, M. Faster creatinine assay in the Abbott Spectrum System. Cl in . Chem., 33(8):1466-1467, 1987.
7. KNAPP, M.L. & MAYNE, P.D. Development ofan automated kinetic Jaffé method designed to minimise bilirribin interference in plasma creatinine
assays. Clin. Chim. ACla, 168:239-246, 1987.
8. KOSTlR, J.V. & SONKA, J. Creatinine estimation in blood serum: new method. Biochim, Biophys. Acla, 8:86-89, 1952.
9. KROLL, M.H.; ROACH, N.A.; POE, B. & ELIN, R.J. Mechanism of interference with the Jaffé reaction for creatinine. Clin. Chem., 33(7):11291132, 1987.
10. LARSEN, K. Creatinine assay by a reaction-kinetic principie. Clin. Chim. Aeta, 41:209-217, 1972.
11. LUSTGARTEN, l.A. & WENK, R.E. Simple, rapid, kinetic method for serum creatinine measurement. Clin. Chem., 18:1419-1422, 1972.
12. MARTlNEZ, E. & DOOLAN, P .D. Determination of creatinine in small quantities of plasma. Observations on two methods. Clin. Chem., 6:233-242, 1960.
13. MCLEAN, M.H.; GALLWAS, r. & HENDRIXSON, M. Evaluation of an automated creatinase creatinine procedure. Clin. Chem., 19:623-625,
1973.
14. MITCHELL, R.l. Improved method for specific determination of creatinine in serum and urine. Clin. Chem., 19:408-410, 1973.
15. PATEL, C.P. & GEORGE, R.C. Liquid chromatographic determination of creatinine in serum and urine. Ana/. Chem., 53:734-735,1981.
16. RAPOPORT,A. & MUSDAN, H. Endogenous creatinine clearance and serum creatinine in the clinícal assessment of kidney function. Cano medo Assoc. i., 99:149-156,1968.
17. TAUSSKY, H. A procedure increasing the specificity of the Jaffé reaction for determination of creatinine in urine and plasma. Clin. Chim. Aeta, 1:210-224, 1956.
18. VASILIADES, J. Reaction of alkaline sodium picrate with creatinine: L Kinetics and mechanism of formation of the mono-creatinine picric acid
complexo Clin. Chem., 22(10):1664-1671, 1976.
19. WATKINS, RE; FELDKAMP, C.S.; THIBERT, R.J. & ZAK, B. Interesting interferences in a direct serum creatinine reaction. Mieroehem, l., 21:370-384, 1976.
20. YATZIDIS, H. New method for direct determination of "true" creatmme. Clin. Chem., 20(9):1131-1134,1974.
Este trabalho está licenciado sob uma licença Creative Commons Attribution 4.0 International License.
Copyright (c) 1988 Lucia N. Castilho, Mary M. Yuki, Marina Y. N. Oda, Heidi P. Martins
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