Prevalence and antimicrobial susceptibility profile of Enterococcus spp isolated from frozen chicken carcasses
PDF

Keywords

Enterococcus
resistance
antimicrobials
chicken carcasses

How to Cite

1.
Ristori CA, Rowlands REG, Bergamini AMM, Lopes GISL, Paula AMR de, Oliveira MA de, Lima M de J de C, Tegani LS, Watanabe AH, Jakabi M, Zanella RC. Prevalence and antimicrobial susceptibility profile of Enterococcus spp isolated from frozen chicken carcasses. Rev Inst Adolfo Lutz [Internet]. 2012 Feb. 1 [cited 2024 May 13];71(2):237-43. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/32420

Abstract

Prevalence and antimicrobial susceptibility of Enterococcus spp. were evaluated in 360 frozen unseasoned chicken carcasses samples collected from September 2004 to June 2006 from the retail stores in São Paulo State, Brazil. Enterococcus spp. was isolated from all analyzed samples, and 1,332 strains were identified from them. Among the ten identified species, the predominance of E. faecalis, E. gallinarum, E. casseliflavus and E. faecium was occurred. All of the Enterococci strains showed some degree of resistance to the nine antimicrobials utilized in the study. The percentages of antimicrobial resistance were: 89.2% for tetracycline, 91.4% for quinupristin-dalfopristin, 83.5% for erythromycin, 65% for ciprofloxacin, 55.4% for chloramphenicol, 6.5% for linezolid, 2.3% for vancomycin, 2.3% for teicoplanin and 0.2% for ampicillin. The occurrence of the high level resistance to amynoglicosides (HLR-A) was detected in 57.4% of the isolates. E. faecalis and E. faecium species, which are considered as important agents in nosocomial infections, showed resistance to eight and seven antibiotics, respectively.
https://doi.org/10.53393/rial.2012.71.32420
PDF

References

1. Teixeira LM, Facklam RR. Enterococcus. In: Murray PR, Baron EJ, Jorgensen JH, Pfaller MA, Yolken RH (eds.). Manual of Clinical Microbiology. 8. ed. Washington, DC: American Society for Microbiology; 2003. p. 422-33.

2. Malani PN, Kauffman CA, Zervos MJ. Enterococcal disease, epidemiology, and treatment. In: Gilmore MS, Clewell DB, Courvalim P, Dunny GM, Murray BE, Rice LB (eds.). The enterococci: pathogenesis, molecular biology, and antibiotic resistance. Washington, DC: American Society for Microbiology; 2002. p. 385-408.

3. Edmond MB, Wallace SE, McClish DK, Pfaller MA, Jones RN, Wenzel RP. Nosocomial bloodstream infections in United States hospitals: a three-year analysis. Clin Infect Dis. 1999;29:239-44.

4. Angulo FJ, Nargund VN, Chiller TC. Evidence of an association between use of anti-microbial agents in food animals and anti-microbial resistance among bacteria isolated from humans and the human health consequences of such resistance. J Vet Med B Infect Dis Vet Public Health. 2004;51(8/9):374-9.

5. Murray BE. Diversity among multidrug-resistant enterococci. Emerg Infect Dis. 1998;4:37-47.

6. Bager F, Madsen M, Christensen J, Aarestrup FM. Avoparcin used as a growth promoter is associated with the occurrence of vancomycin-resistant Enterococcusfaecium on Danish poultry and pig farms. Prev Vet Med. 1997; 31(1-2):95-112.

7. Commission Directive 97/6/EC. January 30, 1997, amending Council Directive 70/524/EEC concerning additives in feedingstuffs. Official Journal of the European Communities. 1997;35:11-3.

8. Brasil. Ministério da Agricultura, Pecuária e Abastecimento – MAPA. Lista de aditivos autorizados uso na alimentação animal no Brasil. Atualizada em 03 dez 2008. [acesso 2011 mar 2]. Disponível em: [http://www.agricultura.gov.br/animal/qualidade-dos-alimentos/aditivos-autorizados].

9. Diaz Granados CA, Zimmer SM, Klein M, Jernigan JA. Comparison of mortality associated with vancomycin-resistant and vancomycin-susceptible enterococcal bloodstream infections: a meta-analysis. Clin Infect Dis. 2005;41:327-33.

10. Huang SS, Datta R, Platt R. Risk of acquiring antibiotic-resistant bacteria from prior room occupants. Arch Intern Med. 2006;166:1945-51.

11. Hammerum AM, Lester CH, Heuer OE. Antimicrobial-resistant enterococci in animals and meat: a human health hazard? Foodborne Pathog Dis. 2010;7(10):1137-46.

12. Kotzamanidis C, Zdragas A, Kourelis A, Moraitou E, Papa A, Yiantzi V, et al. Characterization of vanA-type Enterococcus faecium isolates from urban and hospital wastewater and pigs. J Appl Microbiol. 2009;107(3):997-1005.

13. Haenni M, Saras E, Châtre P, Meunier D, Martin S, Lepage G, et al. VanA in Enterococcus faecium, Enterococcus faecalis, and Enterococcus casseliflavus detected in French cattle. Foodborne Pathog Dis. 2009;6(9):1107-11.

14. López M, Sáenz Y, Rojo-Bezares B, Martínez S, del Campo R, Ruiz-Larrea F, et al. Detection of vanA and vanB2-containing enterococci from food samples in Spain, including Enterococcus faecium strains of CC17 and the new singleton ST425. Int J Food Microbiol. 2009;133(1-2):172-8.

15. Dalla Costa LM, Souza DC, Martins LTF, Zanella RC, Bandilleone MC, Bokerman S, et al. Vancomycin-resistant Enterococcus faecium: first case in Brazil. Braz J Infect Dis. 1998;2:160-3.

16. Zanella RC, Valderato F, Lovgren M, Tyrrel GJ, Bokermann S, Almeida SC, et al. First confirmed case of vancomycin-resistant Enterococcus faecium with vanA phenotype from Brazil: isolation from meningitis case in São Paulo. Microb Drug Resist. 1999;5:159-62.

17. Zanella RC, Brandileone MC, Bokerman S, Almeida SC, Valdetaro F, Vitório F, et al. Phenotypic and genotypic characterization of Van A Enterococcus isolated during the first nosocomial outbreak in Brazil. Microb Drug Res. 2003;9:283-91.

18. Gales AC, Sader HS, Ribeiro J, Zoccoli C, Barth A, Pignatari AC. Antimicrobial susceptibility of Gram-positive bacteria isolated in Brazilian hospitals participating in the SENTRY Program (2005–2008). Braz J Infect Dis. 2009;13(2):90-8.

19. Moretti ML, Oliveira Cardoso LG, Levy CE, Nowakosky A, Bachur LF, Bratfich O, et al. Controlling a vancomycin-resistant enterococci outbreak in a Brazilian teaching hospital. Eur J Clin Microbiol Infect Dis. 2011;30(3):369-74.

20. Boyce JM. Vancomycin-resistant Enterococcus. Detection, Epidemiology and Control Measures. Infect Dis Clin North Am. 1997;11:367-84.

21. Clinical and Laboratory Standards Institute (CLSI). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard M100-S16. Wayne: Clinical and Laboratory Standards Institute; 2006.

22. Clinical and Laboratory Standards Institute (CLSI). Performance Standards for Antimicrobial Susceptibility Testing. Approved standard M7-A7. Wayne: Clinical and Laboratory Standards Institute; 2006.

23. Hayes JR, English LL, Carter PJ, Proescholdt T, Lee KY, Wagner DD, et al. Prevalence and antimicrobial resistance of Enterococcusspecies isolated from retail meats. Appl Environ Microbiol. 2003;69(12):7153-60.

24. McGowan LL, Jackson CR, Barrett JB, Hiott LM, Fedorka-Cray P. Prevalence and antimicrobial resistance of enterococci isolated from retail fruits, vegetables, and meats. J Food Prot. 2006;69(12):2976-82.

25. Gomes BC, Esteves CT, Pallazz IC, Darini AL, Felis GE, Sechi LA, et al. Prevalence and characterization of Enterococcus spp. isolated from Brazilian foods. Food Microbiol. 2008;25(5):668-75.

26. Fracalanzza SAP, Scheidegger EMD, Santos PF, Leite PC, Teixeira LM. Antimicrobial resistance profiles of enterococci isolated from poultry meat and pasteurized milk in Rio de Janeiro, Brazil. Mem Inst Oswaldo Cruz. 2007;102(7):853-9.

27. Miranda JM, Guarddon M, Mondragon A, Vázquez BI, Fente CA, Cepeda A, et al. Antimicrobial resistance in Enterococcus spp. strains isolated from organic chicken, conventional chicken and turkey meat: a comparative survey. J Food Prot. 2007;70(4):1021-4.

28. Pavia M, Nobile CG, Salpietro L, Angelillo IF. Vancomycin resistance and antibiotic susceptibility of enterococci in raw meat. J Food Prot. 2000;63(7):912-5.

29. Ike Y, Tanimoto K, Ozawa Y, Nomura T, Fujimoto S, Tomita H. Vancomycin-resistant enterococci in imported chickens in Japan. Lancet. 1999;353:1854.

30. Xavier DB, Bernal FEM, Titze-de Almeida R. Absence of VanA- and VanB- containing enterococci in poultry raised on nonintensive production faros in Brazil. Appl Environ Microbiol. 2006;72(4):3072-3.

31. Oliveira JS, Zanine AM, Santos EM. Uso de aditivos na nutrição de ruminantes. RedVet. 2005; 6(11). [acesso 2006 jun 25]. Disponível em: [http://www.veterinaria.org/revistas/redvet/n111105.html].

32. Chopra I, Roberts M. Tetracycline antibiotics: mode of action, applications, molecular biology and epidemiology of bacterial resistance. Microbiol Mol Biol Rev. 2001;65(2):232-60.

33. U.S. Food and Drug Administration (FDA). (1999). Guidance for industry, consumer-directed broadcast advertisements. U.S. Food and Drug Administration, U.S. Department of Health and Human Services. [acesso 2003 ago 2]. Disponível em: [http://www.fda.gov/cder].

34. Manzella JP. Quinupristin-Dalfopristin: a new antibiotic for severe Gram-Positive Infections. Am Fam Physician. 2001;64(11):1863-6.

35. McDermott PF, Cullen P, Hubert SK, McDermott SD, Bartholomew M, Simjee S, et al. Change in antimicrobial susceptibility of native in chickens fed virginiamycin. Appl Environ Microbiol. 2005;71(9):4986-91.

36. Rende-Fournier R, Leclercq R, Galimand M, Duval J, Courvalin P. Identification of the satA gene encoding a streptogramin A acetyltransferase in Enterococcus faecium BM4145. Antimicrob Agents Chemother. 1993;37(10):2119-25.

37. Hayes JR, Wagner DD, English LL, Carr LE, Joseph SW. Distribution of streptogramin resistance determinants among Enterococcus faecium from a poultry productions environment of the USA. J Antimicrob Chemother. 2005;55(1):123-6.

38. Bimingham MC, Rayner CR, Meagher AK, Flavin SM, Batts DH, Chentag JJ. Linezolid for the treatment of multidrug-resistant, gram-positive infections: experience from a compassionate-use program. Clin Infect Dis. 2003;36(2):159-68.

39. Scheetz MH, Knechtel SA, Malczynski M, Postelnick MJ, Qi C. Increasing incidance of linezolid-intermediate or-resistant, vancomycin-resistant Enterococcus faecium strains parallels increasing linezolid consumption. Antimicrob Agents Chemother. 2008;52(6):2256-9.

40. Miguel MAL, Teixeira LM, Noleto ALS. Characterization of Enterococcus strains isolated from ready-to-eat foods. Rev Microbiol. 1995;26:121-4.

41. Santos BHC, Souza EL, Oddi IC. Presença de Enterococcus sp. em alimento enteral e perfil de resistência a antimicrobianos. Rev Bras Anal Clin. 2004;36:47-56.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright (c) 2012 Instituto Adolfo Lutz Journal

Downloads

Download data is not yet available.