Keywords
stress testing
high performance liquid chromatography
pharmaceutical drug validation
How to Cite
Abstract
Fenoterol hydrobromide is a β2-adrenergic agonist agent used for asthma and chronic obstructive pulmonary disease treatment. HPLC methodology was developed and validated for quantitative determination of fenoterol hydrobromide. The methodology was achieved by using a reversed-phase C18 column, (150 mm × 3.9 mm i.d., 5 μm) Thermo. The mobile phase was consisted of acetonitrile: water (30:70, v/v) with 0,1% triethylamine, pH adjusted to 5.0 with formic acid and flow rate of 1.0 mL.min-1 with UV detection at 276 nm. The concentration range was from 0.025 to 0.15 mg.mL-1, and the correlation coefficient of analytical curve was >0.999. The detection limit and the quantifying limit (QL) were 0.003 mg.mL-1 and 0.012 mg.mL-1, respectively. Intra- and interday relative standard deviations were ≤2.0%. The methodology accuracy showed the percentage mean of 99.53%. The described technique was found to be simple, rapid, precise, accurate and sensitive; the advantages over the others current methodologies are the low-cost and low-polluting conditions. Owing to its simplicity and reliable results, this methodology is suitable to be used in quality control of pharmaceutical drugs containing fenoterol hydrobromide as active component.References
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