Abstract
The determination of urinary iodine is the most widely used biochemical marker to assess their concentrations, assisting in epidemiological studies and clinical medicina. This investigation evaluated the effects of the preservatives use in the urine samples collected into collector flasks before (A) and after (B) breakfast, and in the samples distributed into tubes either containing (C) or without (S) preservative on determining iodine and creatinine concentrations. The creatinine determination was performed to assess the renal function. This analysis included urine samples from 20 male and female individuals aged from 18 to 40 years. Agreements in the iodine concentrations were found in samples (A) showing an intraclass correlation coefficient (ICC) = 0.769 and ICC = 0.724 in tubes (C) and (S), respectively, and besides between (A) CCI = 0.941 and (B) ICC = 0.983. No agreement on the creatinine concentrations in the tubes (C) ICC = 0.369 and (S) ICC = 0.264 at both periods was observed, and no correlation between (A) (ICC = 0.916) and (B) (ICC = 0.998) as well. No effects was detected on the iodine concentrations in urine samples collected at both periods (A) and (B), and using either tube (C) or (S).References
1. Abalovich M, Amino N, Barbour LA, Cobin RH, De Groot L, Glinoer D, et al. Management of thyroid dysfunction during pregnancy and postpartum: an Endocrine Society Clinical Practice Guideline. J Clin Endocrinol Metab. 2007;92(Suppl 8):S1-S47.
2. World Health Organization. Assessment of iodine deficiency: disorders and monitoring their elimination. A guide for programme managers. Genebra: WHO; 2008.
3. Duarte GC, Tomimori EK, de Camargo RY, Catarino RM, Ferreira JE, Knobel M, et al. Excessive iodine intake and ultrasonographic thyroid abnormalities in schoolchildren. J Pediatr Endocinol Metab. 2009;22(4):327-34.
4. Duarte GC, Tomimori EK, Borioli RA, Ferreira JE, Catarino RM, Camargo RYA, et al. Avaliação ultrassonográfica da tireoide e determinação da iodúria em escolares de diferentes regiões do estado de São Paulo. Arq Bras Endocrinol Metab. 2004;48(6):842-8.
5. Hollowell JG, Staehling NW, Hannon WH, Flanders DW, Gunter EW, Maberly GF, et al. Iodine nutrition in the Unites States. Trends and public health implications: iodine excretion data from National Health and Nutrition Examination Surveys I and III (1971-1974 and 1988-1994). J Clin Endocrinol Metab. 1998;83:3401-8.
6. Benhaim Rochester D, Davies TF. Increased risk of Graves’ disease after pregnancy. Thyroid. 2005;15:1287.
7. Maeda MYS, Santos RTM, Catarino RM, Ferreira JE, Minazzi-Rodrigues RS, Mello MRPA. Estudo da concentração de iodo na urina e no sal de cozinha na população do entorno do Polo Petroquímico de Capuava/Maúa-São Paulo. Rev Bras Epidem, número especial, versão eletrônica. set 2008.
8. Delange F, Benoist B, Pretell E, Dunn JT. Iodine deficiency in the world: Do we stand at the turn of the century? Thyroid. 2001;11(5):437-47.
9. Dunn JT. Iodine deficiency: the next target for elimination. N Engl J Med. 1992;326:267-8.
10. Smits G, Govaerts C, Nubourgh I, Pardo L, Vassart G, Costagliola S. Lysine 183 and glutamic acid 157 of the TSH receptor: two interacting residues with a key role in determining specificity toward TSH and human CG. Molec Endocrinol. 2002;16:722-35.
11. Pretell E, Delange F, Hostalek U, Corigliano S, Barreda L, Higa AM, et al. Iodine nutrition improves in Latin America. Thyroid. 2004;14(8):590-9.
12. International Council for the Control of Iodine Deficiency Disorders. Current IDD status database [acesso 2006 nov]. Disponível em: [http://www.iccidd.org].
13. Dunn JT. Guarding our nation’s thyroid health. J Clin Endocrinol Metab. 2002;87:486-8.
14. Konno N, Makita H, Yuri K, Iizuka N, Kawasaki. Association between dietary iodine intake and prevalence of subclinical hypothyroidism in the coastal regions of Japan. J Clin Endocrinol Metab. 1994;78:393-7.
15. Zimmermann MB, Ito Y, Hess SY, Fujieda K, Molinari L. High thyroid volume in children with excess dietary iodine intakes. Am J Clin Nutr. 2005;81:840-4.
16. Zimmermann MB. Iodine requirements and the risks and benefits of correcting iodine deficiency in populations. J Trace Elem Med Biol. 2008;22:81-92.
17. Burrow GN: Thyroid function and hyperfunction during gestation. Endocr Rev. 1993;14:194.
18. World Health Organization/International Council for the Control of the Iodine Deficiency Disorders/United Nations Childrens Fund (WHO/ICCIDD/UNICEF). Assessment of the iodine deficiency disorders and monitoring their elimination. 2. ed. Genebra: WHO; 2007.
19. Pestalozzi MSC, Nussenzveig I, Bueno IDG, Barros RT, Bacci L, Machado MM. Índice proteína/creatinina em amostra isolada de urina para avaliação da proteinúria de 24 horas. Rev Hosp Clín Fac Med Univ São Paulo. 1989;44(2):58-62.
20. Bottini PV, Afaz SH, Silveira S, Garlipp CR. Utilização da relação albumina/creatinina no diagnóstico de microalbuminúria. J Bras Patol Med Lab. 2005;41(2):99-103.
21. Als C, Helbling A, Peter K, Haldimann M, Zimmerli B, Gerber H. Urinary iodine concentration follows a circadian rhythm: A study with 3,023 spot urine samples in adults and children. J Clin Endocrinol Metab. 2000;85:1367-9.
22. Andersen S, Karmisholt J, Pedersen KM, Laurberg P. Reliability of studies of iodine intake and recommendations for number of samples in groups and in individuals. Br J Nutr. 2007;26:1-6.
23. World Health Organization. Recommended iodine levels in salt and guidelines for monitoring their adequacy and effectiveness. Fifth Report on World Nutrition. Genebra: WHO; mar 2004.
24. Associação Brasileira de Normas Técnicas (ABNT). Comitê Brasileiro de Análises Clínicas e Diagnóstico in vitro. Laboratório clínico: requisitos e recomendações.
25. Jaffé, M. Über den Niederschlag, welchen Pikrinsäure in normalen Harn erzeugt und über eine neue Reaction des Kreatininis. Z Phys Chem. 1886;10:391-400.
26. ICCIDD: Iodine requeriments in pregnancy and infancy. IDD Newsletter. 2007;23(1):1-2.
27. Sandell EB, Kolthoff IM. Micro determination of iodide by a catalytic method. Mikrochim Acta. 1937;1:9-25.
28. Pino S, Fang S-L, Braverman LE. Ammonium persulfate: A safe alternative oxidizing reagent for measuring urinary iodine. Clin Chem. 1996;42(2):239-43.
29. Borioli RA, Ferreira JE, Catarino RM. Importância da determinação de iodo urinário para Saúde Pública. J Bras Patol. 2002;38(3):82.
30. Ferreira JE, Catarino RM, Santos RTM. Comparison of the methodologies in tube and microplate for determination of urinary iodine. Clin Chem Lab Med. 2008;46(Suppl1):M118.
31. Freitas C, Grimaldi-Campos RA, Rodrigues-Silva MA, Panachão MRI, Moraes JC, Waissmann W, et al. Can living in the surroundings of a petrochemical complex be a risk factor for autoimmune thyroid disease?. Env Res. 2010;110(1):112-7.
32. Tonissi AP, Ferreira JE, Costa EAS, Catarino RM, Santos RTM. Estudo da validação do método de iodo urinário: resultados preliminares. Bol Inst Adolfo Lutz. 2007;17(1/2):1-100.
33. Pardete LVH, Hardjowasito W, Gross R, Dillon DH, Totoprajogo OS, Yosoprawoto M. Urinary iodine excretion is the most appropriate outcome indicator for iodine deficiency at field conditions at district level. J Nutr. 1998;128(7):1122-6.
34. Dunn JT, Crutchfield HE, Gutekunst R, Dunn AD. Methods for measuring iodine in urine. Wageningen: Internacional Council for Control of Iodine Deficiency Disordens; 1993.
35. Knudsen N, Christiansen E, Brandt-Chritiansen M, Nygaard B, Perrild H. Age- and sex- adjusted iodine/creatinine ratio. A new standart in epidemiological surveys? Evaluation of three different estimates of iodine excretion based on casual urine samples and comparison to 24 h values. Eur J Clin Nutr. 2000;54:361-3.
36. Busnardo B, Nacamulli D, Zambonin L, Mian C, Piccolo M, Girelli ME. Restricted intraindividual urinary iodine concentration variability in nonfasting subjects. Eur J Clin Nutr. 2006;60(3):421-5.
37. Soldin OP. Controversies in urinary iodine determinations. Clin Biochem. 2002;35:575-9.
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