Emprego de casca de arroz como adsorvente para execução de técnica de MSPD para determinar aflatoxinas em cebolas

Ana Paula Moura Guimarães Carvalho; Helen Cristina dos Santos Hackbart; Michele Moraes de Souza; Eliana Badiale-Furlong

doi:10.53393/rial.2012.71.32476

Vol. 71 No. 4 (2012), ORIGINAL ARTICLE

Vol. 71 No. 4 (2012)

Use of rice husk as an adsorbent for performing MSPS technique for determining the aflatoxins occurrence in onion

ORIGINAL ARTICLE

https://doi.org/10.53393/rial.2012.71.32476

Published 2012-12-01

Ana Paula Moura Guimarães Carvalho⁺⁻
Helen Cristina dos Santos Hackbart⁺⁻
Michele Moraes de Souza⁺⁻
Eliana Badiale-Furlong⁺⁻

Ana Paula Moura Guimarães Carvalho

Universidade Federal do Rio Grande, Escola de Química e Alimentos, Laboratório de Micotoxinas e Ciência de Alimentos, Rio Grande, RS

Helen Cristina dos Santos Hackbart

Universidade Federal do Rio Grande, Escola de Química e Alimentos, Laboratório de Micotoxinas e Ciência de Alimentos, Rio Grande, RS

Michele Moraes de Souza

Universidade Federal do Rio Grande, Escola de Química e Alimentos, Laboratório de Micotoxinas e Ciência de Alimentos, Rio Grande, RS

Eliana Badiale-Furlong

Universidade Federal do Rio Grande, Escola de Química e Alimentos, Laboratório de Micotoxinas e Ciência de Alimentos, Rio Grande, RS

PDF (Português (Brasil))

Keywords

MSPD
HPLC-FD
HPTLC

How to Cite

1.

Carvalho APMG, Hackbart HC dos S, Souza MM de, Badiale-Furlong E. Use of rice husk as an adsorbent for performing MSPS technique for determining the aflatoxins occurrence in onion. Rev Inst Adolfo Lutz [Internet]. 2012 Dec. 1 [cited 2024 Jul. 22];71(4):630-7. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/32476

Abstract

The simultaneous determination of aflatoxins (AFLA) B1, B2, G1 and G2 was investigated in onions by means of matrix solid-phase dispersion (MSPD) extraction methodology, and using rice husk as adsorbent element. The AFLA identification and quantification were carried out by using high efficiency thin layer chromatography (HPTLC) and high efficiency liquid chromatography coupled to a fluorescence detector (HPLC-FD). The best adsorbent was the compound constituted by the rice husk:celite mixture (1:1) in a 1:1 ratio with the mass of the sample, and employing the mixture chloroform:methanol (5:25) as eluent. The methodology showed the limit of detection ranging from 0.05 to 1 μg.kg-1, and of the accuracy from 78 to 93%; and variation coefficients ranged from 11 to 14%. After being validated, the methodology was tested on its applicability in determining the aflatoxins occurrence in onion samples, which were classified according to the standard MAPA. Aflatoxin contamination was found in 43% of analyzed samples with a maximum content of 90 μg.kg-1 of AFLAB2.

https://doi.org/10.53393/rial.2012.71.32476

PDF (Português (Brasil))

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