Pseudomonas aeruginosa: the occurrence and the antimicrobial susceptibility of bacteria isolates from treated water samples utilized for preparing dialysis solution
Vol. 70 No. 4 (2011), ORIGINAL ARTICLE
Vol. 70 No. 4 (2011)

Pseudomonas aeruginosa: the occurrence and the antimicrobial susceptibility of bacteria isolates from treated water samples utilized for preparing dialysis solution

ORIGINAL ARTICLE
https://doi.org/10.53393/rial.2011.v70.32512
Published 2011-04-01
Jacqueline Tanury Macruz Peresi
Instituto Adolfo Lutz, Centro de Laboratório Regional de São José do Rio Preto, São José do Rio Preto, SP
Ivete Aparecida Zago Castanheira de Almeida
Instituto Adolfo Lutz, Centro de Laboratório Regional de São José do Rio Preto, São José do Rio Preto, SP
Inara Siqueira de Carvalho Teixeira
Instituto Adolfo Lutz, Centro de Laboratório Regional de São José do Rio Preto, São José do Rio Preto, SP
Sonia Izaura de Lima e Silva
Instituto Adolfo Lutz, Centro de Laboratório Regional de São José do Rio Preto, São José do Rio Preto, SP
Elisabete Cardiga Alves
Instituto Adolfo Lutz, Centro de Laboratório Regional de São José do Rio Preto, São José do Rio Preto, SP
Denise Fusco Marques
Instituto Adolfo Lutz, Centro de Laboratório Regional de São José do Rio Preto, São José do Rio Preto, SP
Aparecida Klai Ribeiro
Instituto Adolfo Lutz, Centro de Laboratório Regional de São José do Rio Preto, São José do Rio Preto, SP
PDF (Português (Brasil))

Keywords

Pseudomonas aeruginosa
treated water for dialysis solution
bacterial resistance
bacterial contamination

How to Cite

1.
Peresi JTM, Almeida IAZC de, Teixeira IS de C, Lima e Silva SI de, Alves EC, Marques DF, Ribeiro AK. Pseudomonas aeruginosa: the occurrence and the antimicrobial susceptibility of bacteria isolates from treated water samples utilized for preparing dialysis solution. Rev Inst Adolfo Lutz [Internet]. 2011 Apr. 1 [cited 2024 Nov. 22];70(4):542-7. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/32512
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver

Download Citation

Endnote/Zotero/Mendeley (RIS) BibTeX

Abstract

This study reports the presence of Pseudomonas aeruginosa in samples of treated water used in the dialysis solution in eight Renal Replacement Therapy Units in four cities of the northwest region of São Paulo state; and the isolated strains were analyzed on the resistance profile by antimicrobial susceptibility testing. From 2000 to 2009, 508 samples were analyzed following Standard Methods for the Examination of Water and Wastewater (2005). P. aeruginosa was isolated from 43 (8.5%) samples, and 55.8, 23.3 and 20.9% of them were from white room, post-osmosis/deionizer point and yellow room of different units, respectively. The A Unit water samples have showed the highest bacterium isolation frequency (53.5%); and the detection of the highest number of contaminated samples (16.7%) occurred in 2004. Of all the 43 isolates, 38 were submitted to the antimicrobial susceptibility testing; an intermediate resistance to gentamicin in one sample (2.6%) and resistance to aztreonam and ticarcillin / clavulanic acid in another (2.6%) were identified. Considering the implications of P. aeruginosa to the public health, this survey of bacterium should be included in monitoring water quality for hemodialysis and this procedure might be an indicator for evidencing deficiencies on the water distribution network control.

https://doi.org/10.53393/rial.2011.v70.32512
PDF (Português (Brasil))

References

1. Sociedade Brasileira de Nefrologia. [acesso 2011 Ago 05]. Disponível em: [http://www.sbn.org.br/leigos/pdf/censo2010finalizado_leigos.ppt].

2. Arvanitidou M, Spaia S, Katsinas C, Pangidis P, Constantinidis T, Katsouyannopoulus V, et al. Microbial quality of water and dialysate in all haemodialysis centres of Greece. Nephrol Dial Transplant. 1998;13:949-54.

3. Silva AMM, Martins CTB, Ferraboli R, Jorgetti V, Romão Jr JE. Revisão/Atualização em diálise: água para hemodiálise. J Bras Nefrol. 1996;18(2):180-8.

4. Favero MS, Alter MJ, Bland LA. Dialysis ¬associated infections. In: Bennett JV, Brachman PS, Sanford JP (eds.). Hospital Infections. Boston: Little Brown; 1992. p. 375-403.

5. Du Molin GC, Coleman EC, Hedley-Whyte J. Bacterial colonization and endotoxin content of a new renal dialysis water system composed of acrylonitrile butadiene styrene. Appl Env Microbiol. 1987;53:1322-6.

6. Morin P. Identification of the bacteriological contamination of a water treatment line used for haemodialysis and its disinfection. J Hosp Infect. 2000;45:218-24.

7. Wang SA et al. An outbreak of gram-negative bacteremia in hemodialysis patients traced to hemodialysis machine waste drain ports. Infect Control Hosp Epidemiol.1999;20(11):746-51.

8. Magalhães M, Doherty C, Govan JRW, Vandamme P. Polyclonal outbreak of Burkholderia cepacia complex bacteraemia in haemodialysis patients. J Hosp Infec. 2003;54:120-3.

9. Yan H, Shi L, Alam MJ, Li L, Yang L, Yamasaki S. Usefulness of Sau-PCR for molecular epidemiology of nosocomial outbreaks due to Burkholderia cepacia which occurred in a local hospital in Guangzhou, China. Microbiol Immunol. 2008;52:283–6.

10. Pisani B, Simões M, Prandi MA, Rocha MMM, Gonçalves CR, Vaz TMI, et al. Surto de bacteremia por Pseudomonas aeruginosa na unidade de hemodiálise de um hospital de Campinas, São Paulo, Brasil. Rev Inst Adolfo Lutz. 2000;59(1/2):51-6.

11. Beck-Sague CM, Jarvis WR, Bland LA, Arduino MJ, Aguero SM, Verosic G. Outbreak of Gram – Negative Bacteremia and Pyrogenic Reactions in a Hemodialysis Center. Am J Nephrol. 1990;10:397-403.

12. Welbel SF, Schoendorf K, Bland LA, Arduino MJ, Groves C, Schable B et al. An outbreak of gram-negative bloodstream infections in chronic hemodialysis patients. Am J Nephrol. 1995; 15:1-4.

13. Rudnick JR, Arduino MJ, Bland LA, Cusick L, McAllister SK, Agüero SM et al. An outbreak of pyrogenic reactions in chronic hemodialysis patients associated with hemodialyzer reuse. J Artif Organs. 1995;19:289-94.

14. Lincopan N, Trabulsi LR. Pseudomonas aeruginosa. In: Trabulsi LR, Alterthum F, editores. Microbiologia. São Paulo: Editora Atheneu; 2008. p. 369-81.

15. Levin ASS, Arruda EAG. Infecções por Pseudomonas. In: Veronesi R, Foccacia R, editores. Tratado de infectologia. São Paulo: Editora Atheneu; 1996. p.739-45.

16. Brasil. Agência Nacional de Vigilância Sanitária. Resolução Diretoria Colegiada Nº154, de 15 de junho de 2004. Estabelece o Regulamento Técnico para o funcionamento dos serviços de Diálise. Diário Oficial [da] União. Brasília, DF, 17 jun 2004. Republicada em 31 mai. 2006. [acesso 2011 Jul 01]. Disponível em: [http://e-legis.anvisa.gov.br/leisref/public/showAct.php?].

17. American Public Health Association. Standard Methods for Examination of Water and Wastewater. 21st ed. Washington: APHA; 2005.

18. Palleroni NJ. Genus I. Pseudomonas. In: Brenner DJ, Krieg NR, Staley JT, Garrity GM. (eds.). Bergey’s manual of systematic bacteriology. East Lansing: Springer; 2005, p.323-79.

19. Bauer AW, Kirby Wmm, Sherris JSC, Turk M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966;45(4):493-6.

20. Clinical and Laboratory Standards Institute - CLSI. Performance Standards for Antimicrobial Susceptibility Testing. Twentieth Informational Supplement M100-S20, 30(1), Wayne; 2011.

21. Brasil. Agência Nacional de Vigilância Sanitária. Resolução Diretoria Colegiada ANVISA nº 6, de 14/02/2011. Altera a Resolução RDC nº 154, de 15/06/2004, que estabelece o Regulamento Técnico para o funcionamento dos serviços de Diálise, republicada em 31/05/2006. Diário Oficial [da] União. Brasília, DF, 15 fev. 2006. Seção 1. p.55.

22. Arvanitidou M, Vayona A, Spanakis N, Tsakris A. Occurrence and antimicrobial resistance of Gram-negative bacteria isolated in haemodialysis water and dialysate of renal units: results of a Greek multicentre study. J Appl Microbiol. 2003;95:80-5.

23. Bugno A, Almodóvar AAB, Pereira TC, Auricchio M. Detecção de bactérias Gram-negativas não fermentadoras em água tratada pára diálise. Rev Inst Adolfo Lutz. 2007;66(2):172-5.

24. Montanari LB, Sartori FG, Cardoso MJO, Varo SD, Pires RH, Leite CQF, et al. Microbiological contamination of a hemodialysis center water distribution system. Rev Inst Med Trop S. Paulo. 2009;51(1):37-43.

25. Simões M, Pires MFC. Água de diálise: ocorrência de leveduras, Pseudomonas aeruginosa e bactérias heterotróficas. Rev Inst Adolfo Lutz. 2004;63(2):224-31.

26. Reis JDP, Faria NC, Filter A. Qualidade bacteriológica da água para hemodiálise do Distrito Federal. Rev Saúde Distr Fed. 1998;9(2):39-43.

27. Ferreira JAB. Diversidade genética, perfil de resistência aos antimicrobianos e produção de biofilme de amostras de Pseudomonas aeruginosa isoladas da água utilizada em unidades de terapia renal substitutiva [dissertação de mestrado]. Rio de Janeiro (RJ): Fundação Oswaldo Cruz; 2009. [acesso 2011 Ago 05]. Disponível em: [http://teses.icict.fiocruz.br/pdf/3551_JoanaAngelicaBarbosaFerreira.pdf ].

28. Vorbeck-Meister I, Sommer R, Vorbeck F, Hörl WH. Quality of water used for haemodialysis: bacteriological and chemical parameters. Nephrol Dial Transplant. 1999;14:666-75.

29. Roth VR, Jarvis WR. Outbreaks of infection and or pyrogenic reactions in dialysis patients. Semin Dial. 2000;13(2):92-6.

30. Smeets E, Kooman J, van der Sande F, Stobberingh E, Frederik P, Claessens P et al. Prevention of biofilm formation in dialysis water treatment systems. Kidney Int. 2003;63:1574-6.

31. Pontoriero G, Pozzoni P, Andrulli S, Locatelli F. The quality of dialysis water. Nephrol Dial Transplant. 2003;18 (suppl 7):vii21-5.

32. Lonnemann, G. The quality of dialysate: an integrated approach. Kidney Int. 2000;76:S112-9.

33. Padoveze MC, Oliveira EL. Bactérias Multi-Resistentes. [acesso 2011 Ago 12]. Disponível em: [http://www.hospvirt.org.br/enfermagem/port/bacterias_multi.html].

34. Laroche E, Pawlak B, Berthe T, Skurnik D, Petit F. Occurrence of antibiotic resistance and class 1, 2 and 3 integrons in Escherichia coli isolated from a densely populated estuary (Seine, France). FEMS Microbiol Ecol. 2009;68(1):118-30.

35. Marion K, Pasmore M, Freney J, Delawari E, Renaud F, Costerton JW, Traeger J. A new procedure allowing the complete removal and prevention of hemodialysis biofilms. Blood Purif. 2005;23(5):339-48.

36. Cappelli G, Riccardi M, Perrone S, Bondi M, Ligabue G, Albertazzi A. Water treatment and monitor disinfection. Hemodial Int. 2006;10(1):13-8.

37. Del Fiol FS, Mattos Filho TR, Groppo FC. Resistência bacteriana. Rev Bras Med. 2000;57(10):85-7.

38. Wenzel RP, Edmond MB. Managing antibiotic resistence. N Engl J Med. 2000;343(26):1961-3.

39. Borges CR, Lascowski KM, Filho NR, Pelayo JS. Microbiological quality of water and dialysate in a hemodialysis unit in Ponta Grossa-PR, Brazil. J Appl Microbiol. 2007;103(5):1791-7.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright (c) 2011 Instituto Adolfo Lutz Journal

Downloads

Download data is not yet available.