Keywords
amebiasis
ELISA
coproantigen
How to Cite
Abstract
The differentiation of the pathogen that cause amebiasis has turned to be relevant since Entamoeba histolytica (pathogenic) has been considered as a distinct species from Entamoeba dispar (non-pathogenic). In the present study E. histolytica antigens were investigated in stool samples from children of São Leopoldo, Rio Grande do Sul, Brazil, by means of a commercially available enzyme-linked immunosorbent assay, ELISA (E. histolytica Test, TechLab Inc., Blacksburg, USA). A total of 262 fecal samples were analyzed by Hoffman, Pons and Janer method, and three samples showed positive result for E. histolytica/E. dispar complex. Of 262 samples, 91 (including those positive on Hoffman, Pons and Janer method) were analyzed by ELISA. The discrepancy of results on coproparasitologic exam and on ELISA was detected as all fecal samples were negative on ELISA, indicating the presence of E. dispar but not of E. Histolytica in those three positive samples on HPJ technique. These data point out the relevance in employing a highly specific technique for performing the identification and differentiation of intestinal amebiasis.References
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