Differenciation between small-pox and vaccinia viruses in tissue cultures.
, ORIGINAL ARTICLE

Differenciation between small-pox and vaccinia viruses in tissue cultures.

ORIGINAL ARTICLE
https://doi.org/10.53393/rial.1962.22.33415
Published 1962-02-01
Marta Irena Malachowska
Do Instituto Adolfo Lutz.
Luis Florencio de Salles Gomes
Do Instituto Adolfo Lutz.
PDF (Português (Brasil))

How to Cite

1.
Irena Malachowska M, Florencio de Salles Gomes L. Differenciation between small-pox and vaccinia viruses in tissue cultures. Rev Inst Adolfo Lutz [Internet]. 1962 Feb. 1 [cited 2025 Jan. 10];22:93-6. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/33415
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver

Download Citation

Endnote/Zotero/Mendeley (RIS) BibTeX

Abstract

The technique of Lindenmann for testing the effect of interferon on viruses grown in tissue cultures was successfully employed in the macroscopic differentiation between discrete lesions of variola and vaccinia viruses grown in cultures of chicken embryo, human kidney and amnion cells. Staining with crystal violet improved the differentiation.

https://doi.org/10.53393/rial.1962.22.33415
PDF (Português (Brasil))

References

1. NOYES, W. F. - A sírnple technic for demonstrating plaque formation with virus of vaccínía, Proc. Soe. Exp. BioI. Med. 83:426-429, 1953.

2. ST. GEME, J. W., Jr. - A símple in vitro assay for vaccinia vírus using tube cultures of human cells. Proc. Soe. Exp. BioI. Med.: 113: 133-135, 1963.

3. KIRN, A. & BRAUNWALD, J. - Titrage du virus vaccinal par Ia méthode des pIages SUl' souche cellulaire KB. 1. Expose de Ia méthode. Ann. Inst. Pasteur (Paris) 103:832-840, 1962.

4. LINDENMANN, J. & GIFFORD, G.E. - Studies of vaccinia virus pIaque formation and its inhibition by interferon. 1. Dynamíes of pIaque formation by vaccinia virus. Vírology 19:283-293, 1963.

5. MAHNEL, H. & MUNZ, E. - Differenzierung von Variola-und Vakzine-virus ín HeLa-und Fl-Zellkulturen. ZbI. Bakt. (Oríg.) 178:149-155,1960.

6. PORTERFIELD, J. S. - A simpIe plaque- -inhíbitíon test for the study of arthropod-borne viruses. Bull. WId. HIth. Org. 22:373-380, 1960.

7. PORTERFIELD, ,J. S. & ALLISON, A.C. - Studies wíth poxviruses by an ímproved plaque technique. Virology 10: 233-244,1960.

8. POSTLETHWAITE, R. - Aplaque techníque for the titration of vaccinia virus in chick embryo cells and some features of vaccinial infection in this system. Virology 10:466-482, 1960.

9. VIEUCHANGE, G., BRION, G. de & GRUEST, J. - Virus varíolique en cultures de cellules. Ann. Inst. Pasteur (Paris) 95:681-693, 1958.

10. YOUNGNER, J. S. - Virus adsorption and plaque formation in monolayer cultures of trypsin-dispersed monkey kidney. J. Immun. 76:288-292, 1956.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright (c) 1962 Instituto Adolfo Lutz Journal

Downloads

Download data is not yet available.