Abstract
A simple method for the determination of calcium or sodium pantothenate and pantothenic acid in pharmaceutical preparations is presented. The property of the mentioned compounds of forming beta-alanine by a acid hydrolysis is used. The beta-alanine formed is purified by paper chromatography and detected with ninhydrin. The stain is eluted and the absorbance measured according to Giri, Radhakrishnam & Vaidyanathan. Hydrolysis conditions were studied as well as the possible interference of other amino-acid which may be present. Several kinds of solvent for development of the chromatogram were also studied.
References
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Copyright (c) 1965 Instituto Adolfo Lutz Journal