Keywords
contamination
natural products
food safety
Cladosporium
How to Cite
Abstract
Cladosporium spp. is a group of dematiaceous food-relevant fungi which are well dispersed in the environment causing food spoilage and poisoning. Considering the importance of fungal contamination, natural drugs to control their growth have become important. Thus, the aim of this study was to evaluate the inhibitory effects of two monoterpenoids, (geraniol and citronellol), against strains of Cladosporium carrioni, C. cladosporioides, and C. oxysporum. Methods: The Minimum Inhibitory Concentration (MIC) and Minimum Fungicide Concentration (MFC) of the drugs were determined by microdilution. The effects of test drugs on mycelial dry weight, conidia germination, and conidiogenesis of Cladosporium spp. were also investigated using a hemacytometer. Respective MIC and MFC values of citronellol varied from 256 to 512 µg/mL, and from 256 to 2048 µg/mL. The MIC and MFC of geraniol varied similarly to citronellol. Conidia germination, mycelial dry weight, and conidiogenesis of Cladosporium spp. were reduced by the test-drugs at 1/2MIC, MIC and 2xMIC (p< 0.05). These measurable cell events are essential for fungal infection and development in foods. The action of citronellol and geraniol against Cladosporium spp. suggest that the drugs may serve as effective agents for controlling fungal contamination and growth in foods.References
1. newell DG, Koopmans M, verhoef L, Duizer E, Aidara-Kane A, Sprong H et al. Food-borne diseases - The challenges of 20 years ago still persist while new ones continue to emerge. Int J Food Microbiol. 2010;139 Suppl 1:S3-15. [DoI: http://dx.doi.org/10.1016/j.ijfoodmicro.2010.01.021].
2. Mendes GRL, Reis tA, Corrêa B, Badiale-Furlong E. Mycobiota and occurrence of Fumonisin B1 in wheat harvested in Southern Brazil. Ciên Rural. 2015;45(6):1050-7. [DoI: http://dx.doi.org/10.1590/0103-8478cr20140005].
3. Bensch K, Braun u, Groenewald JZ, Crous PW. The genus Cladosporium. Stud Mycol. 2012;72(1):1-401. [DoI: http://dx.doi.org/10.3114/sim0003].
4. Altunatmaz SS, Issa G, Aydin A. Detection of airborne psychrotrophic bacteria and fungi in food storage refrigerators. Braz J Microbiol. 2012; 43(4):1436-43. [DoI: 10.1590/S1517-838220120004000027].
5. ogórek R, Lejman A, Pusz W, A Miluch, Miodynska P. Characteristics and taxonomy of Cladosporium fungi. Mikologia Lekarska. 2012.19(2):80-5.
6. Rivas S, Thomas CM. Molecular interactions between tomato and the leaf mold pathogen: Cladosporium fulvum. Annu Rev Phytopathol. 2005;43:395-436. [DoI: http://dx.doi.org/10.1146/annurev.phyto.43.040204.140224].
7. Suwa M, oie S, Furukawa H. Efficacy of disinfectants against naturally occurring and artificially cultivated bacteria. Biol Pharm Bull. 2013;36(3):360-3. [DoI: https://dx.doi.org/10.1248/bpb.b12-00721].
8. Hyldgaard M, Mygind t, Meyer RL. Essential oils in food preservation: mode of action, synergies, and interactions with food matrix components. Front Microbiol. 2012;3:12. [DoI: http:/dx.doi.org/10.3389/fmicb.2012.00012].
9. Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils-a review. Food Chem toxicol. 2008;46(2):446–75. [DoI: https://doi.org/10.1016/j.fct.2007.09.106].
10. Prasad Sn, Muralidhara. neuroprotective effect of geraniol and curcumin in an acrylamide model of neurotoxicity in Drosophila melanogaster: relevance to neuropathy. J Insect Physiol. 2014;60(1):7-16. [DoI: http://dx.doi.org/10.1016/j.jinsphys.2013.10.003].
11. Jeon JH, Lee CH, Lee HS. Food protective effect of geraniol and its congeners against stored food mites. J Food Prot. 2009;72(7):1468-71.
12. Wiseman DA, Werner SR, Crowell PL. Cell cycle arrest by the isoprenoids perillyl alcohol, geraniol, and farnesol is mediated by p21Cip1 and p27Kip1 in human pancreatic adenocarcinoma cells. J Pharmacol Exp ther. 2007;320(3):1163-70. [DoI: https://dx.doi.org/10.1124/jpet.106.111666].
13. Aoudou Y, Léopold tn, Michel JDP, Xavier EF, Moses MC. Antifungal properties of essential oils and some constituents to reduce foodborne pathogen. J Yeast Fungal Res. 2010;1(1):1-8.
14. Brito RG, Santos PL, Quintans JSS, Lucca Júnior W, Araújo AA, Saravananc S, et al. Citronellol, a natural acyclic monoterpene, attenuates mechanical hyperalgesia response in mice: evidence of the spinal cord lamina I inhibition. Chem Biol Interact. 2015;239:111-7. [DoI: http:/dx.doi.org/10.1016/j.cbi.2015.06.039].
15. Semmler M, Abdel-Ghaffar F, Schmidt J, Mehlhorn H. Evaluation of biological and chemical insect repellents and their potential adverse effects. Parasitol Res. 2014;113(1):185-8. [DoI: http:/dx.doi.org/ 10.1007/s00436-013-3641-7].
16. Rasooli I, Abyaneh MR. Inhibitory effect of thyme oils on growth and aflatoxin production by Aspergillus parasiticus. Food Control. 2004;15(6):479-83. [DoI: http:/dx.doi.org/10.1016/j.foodcont.2003.07.002].
17. Lira Mota KS, oliveira Pereira F, oliveira WA, Lima Io, oliveira Lima E. Antifungal activity of Thymus vulgarisL. essential oil and its constituent phytochemicals against Rhizopus oryzae: interaction with ergosterol. Molecules. 2012;17(12):14418-3. [DoI: http:/dx.doi.org/10.3390/molecules171214418].Santos AS, Silva GS, Silva KvS, Lima MIo, Arrua JMM, Lima Eo et al. Antifungal activity of geraniol and citronellol against food-relevant dematiaceous fungi Cladosporium spp. Rev Inst Adolfo Lutz. São Paulo, 2017;76:e1732.
18. Clinical and Laboratory Standards Institute - CLSI Reference Method for Broth Dilution Antifungal Susceptibility testing of Filamentous Fungi; Approved Standard. CLSI document M38-A. Wayne, PA: Clinical and Laboratory Standards Institute; 2002.
19. Klepser ME, Ernst EJ, Ernst ME, Messer SA, Pfaller MA. Evaluation of endpoints for antifungal susceptibility determinations with LY303366. Antimicrob Agents Chemother. 1998;42(6):1387-91.
20. Ernst EJ, Klepser ME, Ernst ME, Messer SA, Pfaller MA. In vitro pharmacodynamic properties of MK-0991 determined by time-kill methods. Mycol. 1999;33(2):75-80.[DoI: http://dx.doi.org/10.1016/S0732-8893(98)00130-8].
21. Liu t, Zhang Q, Wang L, Yu L, Leng W, Yang J, et al. the use of global transcriptional analysis to reveal the biological and cellular events involved in distinct development phases of Trichophyton rubrum conidial germination. BMC genomics. 2007;8:100. [DoI: https://dx.doi.org/10.1186/1471-2164-8-100].
22. oliveira Pereira F, Alves Wanderley P, Cavalcanti viana FA, Batazar de Lima R, Barbosa de Sousa F, oliveira Lima E. Growth inhibition and morphological alterations of Trichophyton rubrum induced by essential oil from Cymbopogon winterianus Jowitt ex Bor. Braz J Microbiol. 2011;42(1):233-42. [DoI: http://dx.doi.org/10.1590/S1517-83822011000100029].
23. Sharma n, tripathi A. Effects of Citrus sinensis (L.) osbeck epicarp essential oil on growth and morphogenesis of Aspergillus niger (L.) van tieghem. Microbiol Res. 2008;163(3):337-44. [DoI: https://dx.doi.org/10.1016/j.micres.2006.06.009].
24. tzortzakis nG, Economakis CD. Antifungal activity of lemongrass (Cympopogon citratus L.) essential oil against key postharvest pathogens. Inn Food Sci Emerg technol. 2007;8(2):253-8. [DoI: https://dx.doi.org/10.1016/j.ifset.2007.01.002].
25. Sartoratto A, Machado ALM, Delarmelina C, Figueira GM, Duarte MCt, Rehder vLG. Composition and antimicrobial activity of essential oils from aromatic plants used in Brazil. Braz J Microbiol. 2004;35(4):275-80. [DoI: http://dx.doi.org/10.1590/S1517-83822004000300001].
26. Shin S, Lim S. Antifungal effects of herbal essential oils alone and in combination with ketoconazole against Trichophyton spp. J App Microbiol. 2004;97(6):1289-96. [DoI: http://dx.doi.org/10.1111/j.1365-2672.2004.02417.x].
27. Pereira Fo, Mendes JM, Lima Io, Mota KS, oliveira WA, Lima Eo. Antifungal activity of geraniol and citronellol, two monoterpenes alcohols, against Trichophyton rubruminvolves inhibition of ergosterol biosynthesis. Pharm Biol. 2015;53(2):228-34. [DoI: http://dx.doi.org/10.3109/13880209.2014.913299].
28. oliveira WA, oliveira Pereira F, Luna CG, Lima Io, Wanderley PA, Lima RB, et al. Antifungal activity of Cymbopogon winterianus jowitt ex bor against Candida albicans. Braz J Microbiol. 2011;42(2):433-41. [DoI: http://dx.doi.org/10.1590/S1517-83822011000200004].
29. Pontin M, Bottini R, Burba JL, Piccoli P. Allium sativumproduces terpenes with fungistatic properties in response to infection with Sclerotium cepivorum. Phytochemistry. 2015;115:152-60. [DoI: http://dx.doi.org/10.1016/j.phytochem.2015.02.003].
30. Dao t, Dantigny P. Control of food spoilage fungi by ethanol. Food Control. 2011; 22(3-4): 360-8. [DoI: http://dx.doi.org/10.1016/j.foodcont.2010.09.019].
31. Seong KY, Zhao X, Xu JR, Güldener u, Kistler HC. Conidial germination in the filamentous fungus Fusarium graminearum. Fungal Genet Biol. 2008;45(4):389-99. [DoI: http://dx.doi.org/10.1016/j.fgb.2007.09.002].
32. Gupta AK, Chaudhry M, Elewski B. Tinea corporis, Tinea cruris, Tinea nigra, and Tinea piedra. Dermatol Clin. 2003;21(3):395-400.
33. Schoeny R. Disinfection by-products: a question of balance. Environ Health Perspect. 2010;118(11):466-7. [DoI: http://dx.doi.org/10.1289/ehp.1003053].
34. Gómez-López vM, Marin A, Medina-Martinez MS, Gil MI, Allende A. Generation of trihalomethanes with chlorine-based sanitizers and impact on microbial, nutritional and sensory quality of baby spinach. Postharvest Biol technol. 2013;85(1):210-7.
35. RIFM EXPERt Panel, Belsito D, Bickers D, Bruze M, Calow P, Greim H, Hanifin JM et al. A toxicologic and dermatologic assessment of cyclic and non-cyclic terpene alcohols when used as fragrance ingredients. Food Chem toxicol. 2008; 46(Suppl 11):S1–S71. [DoI: http://dx.doi.org/10.1016/j.fct.2008.06.085].
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