Keywords
high performance liquid chromatography
stability
margarine
How to Cite
Abstract
With the purpose to brighten up the problems related to the folic acid deficiency, many foods are being enriched. Analytical methodologies are necessary to evaluate the behavior of this vitamin when added into these products. The aim of the work was the validation of methodology for folic acid analysis in enriched margarine. The vitamin was extracted from enriched margarine using KOH solution and the extract was cleaned with trichloroacetic acid. The vitamin was separated in a C18 column, with acid solution and acetonitrile as mobile phase. The ultraviolet detection was at 290nm and the quantification was by external standardization. The methodology was efficient showed recovery rates among 94 and 97%, good repeatability (V.C. 0.5 to 1.4%) and detection and quantification limits were 1.3ng/g and 2.6ng/g, respectively. Shelf life study, during 4 storage months, resulted in loss of great portion about vitamin, approached 55%.
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Copyright (c) 2004 Revista do Instituto Adolfo Lutz
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