Keywords
hydroxyproline
spectrophotometric method
quantitative determination
collagen
canned meat
How to Cite
Abstract
The purpose of validation approach is to assure the adequacy of an analytical method, and it may be performed by either inter - or intralaboratory studies. Accuracy is one of the main factors in the validation process, and it may be evaluated by employing reference samples. For hydroxyproline determination of collagen routine analysis, spectrophotometry methods are used due to the precision,
accuracy, simplicity, low cost and rapidity. The present investigation aimed at the intralaboratory
validation of the official AOAC (Association of Official Analytical Chemists) spectrophotometric
determination of hydroxyproline in canned meat employing the FAPAS/MAFF/UK (Food Analysis
Performance Assessment Scheme / Ministry of Agriculture, Fishries and Food / United Kingdom) as
reference material. The calibration curve showed linearity (r=0.9991) in the applied concentration interval. The method revealed quantification limit of 0.030μg/mL with relative standard deviation of 6.5%, and 0.0075g/100g for the sample. The recovery rates were of 90-95% for three spiked concentrations FAPAS samples. The repeatability rates displayed the CV (variation coefficient) below 4%. Participation in nine series of interlaboratory FAPAS/MAFF proficiency program...
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Copyright (c) 2004 Revista do Instituto Adolfo Lutz
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