A dosagem de hemoglobina A2 pode ser feita a partir de amostra de hemolisado com saponina ?

Raimundo A. G.  Oliveira; Fabiano P.  Falcão; Maria do Socorro G.  Oliveira; Marilena  Oshiro; Orlando C. de O.  Barretto

doi:10.53393/rial.2003.62.34944

Vol. 62 No. 1 (2003), ORIGINAL ARTICLE

Vol. 62 No. 1 (2003)

Could the saponin hemolysate be used for the hemoglobin A2 dosage ?

ORIGINAL ARTICLE

https://doi.org/10.53393/rial.2003.62.34944

Published 2003-04-30

Raimundo A. G. Oliveira⁺⁻
Fabiano P. Falcão⁺⁻
Maria do Socorro G. Oliveira⁺⁻
Marilena Oshiro⁺⁻
Orlando C. de O. Barretto⁺⁻

Raimundo A. G. Oliveira

Universidade Federal do Maranhão

Fabiano P. Falcão

Universidade Federal do Maranhão

Maria do Socorro G. Oliveira

Universidade Federal do Maranhão

Marilena Oshiro

Instituto Adolfo Lutz- São Paulo

Orlando C. de O. Barretto

LIM 23 - Instituto de Psiquiatria , Hospital das Clínicas da FM-USP

pdf (Português (Brasil))

Keywords

Hemoglobin A2
hemolysate
hemoglobinophaties
electrophoresis

How to Cite

1.

Oliveira RAG, Falcão FP, Oliveira M do SG, Oshiro M, Barretto OC de O. Could the saponin hemolysate be used for the hemoglobin A2 dosage ?. Rev Inst Adolfo Lutz [Internet]. 2003 Apr. 30 [cited 2024 Jul. 22];62(1):17-20. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/34944

Abstract

It was made the evalution of the hemoglobin A₂(Hb A₂) determination using four hemolysate types. In the 1st. stage of the evalution thirty eterminations were proceeded for fraction of Hb A₂starting from hemolysate with saponine and starting from carbon tetrachloride, in the 2^ndstage more 40 determinations were analyzed using hemolysate obtained through saponine and with cloroform, however added with potassium cyanide (KCN). The statistical analysis done through test t in the ¹^st, it revealed a
tendency of the results obtained starting from saponine are larger about 0,42% that those obtained starting from carbon tetrachloride (p<0,001). However, when KCN is added in the hemolysate there is no significant difference among the classic hemolisado and with the saponine. Like this, the use of the classic hemolysate
or of the saponine with KCN are indicative for Hb A₂quantitative determination for the method of elution.

https://doi.org/10.53393/rial.2003.62.34944

pdf (Português (Brasil))

References

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2. Fairbanks, V.F; Lee, G.G. Aspectos bioquímicos da hematologia. In: Burtis CA, Ashwood ER, Tietz. Fundamentos de Química Clínica. Rio de Janeiro: Guanabara Koogan, 1998. Cap. 36, p.681-706.

3. Lee, G.R et al. Wintrobe: Hematologia Clínica. São Paulo: Manole, 1998. Cap. 36, pág. 1120.

4. Lukens, J.N. Talassemias e Distúrbios Afins: Distúrbios Quantitativos da Síntese da hemoglobina. In: Lee, G.R. et al. Wintrobe: Hematologia Clínica. São Paulo: Manole, 1998. Cap. 39, p. 1206-1235.

5. Marengo-Rowe, A.J. Rapid electrophoresis and quantitation of humam haemoglobin on celulose acetate. J Clin Pathol, 18: 790-792, 1965.

6. Mazza, U. et al. Clinical and Haematological data in 254 cases of beta-thalassaemias trait in Italy. Br J Haematol 33: 91-99, 1976.

7. Naoum, P.C. Hemoglobinopatias e Talassemias. São Paulo: Sarvier, 1997, pág 171.

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Could the saponin hemolysate be used for the hemoglobin A2 dosage ?

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