Keywords
alpha-lactalbumin
lactoferrin
casein secretory component
epithelial membrane
antigen and carcinoembryonic
How to Cite
Abstract
Four cases of human breast carcinomas were selected and tested for the presence of breast epithelial cell differentiation. Antisera to alpha-lactalbumin, lactoferrin, casein, secretory component, epithelial membrane antigen and carcinoembryonic antigen were employed. The well differentiated tumor was positive for a11 markers tested while the less differentiated one was negative exccpt for alpha-lactalbumin. The intermediate tumors lresented focal staining in the more differentiated areas. A relationship was foun between the presence of markers of breast differentiation and good histological differentiation of tumors. No particular correlation was noted when alpha-lactalbumin was employed.
References
1. BUSSOLA TI, G.; PICH, A. & ALFANI, V. - Immunofluorescence detection of casein in human mammary dysplastic and neoplasic tissues. Yirchows Arcb (AJ,365: 15-21, 1975.
2. CHARPIN, C.; LACHARD, A. & PORREAUSCHNEIDER, N. - Localization of lactoferrin and nonspecific cross-rcacting antigen in human breast carcinomas. Cancer,55: 2612-2617, 1985.
3. CLA YTON, F.; ORDONEZ, N.G.; HANSSEN, G.M. & HANSSEN, H. - Immunoperoxidase localization of lactalbumin in malignant breast neoplasms. Arch. Path. Lab. Med. 106: 268-270, 1982.
4. FORTT, R.W.; ornas. A.R.; WILUAMS, D.; HANSEN, J. & WILUAMS, L - The identification of casein in human breast cancer. Histopathology 3: 395-406, 1979.
5. HEGG, R.; PESTANA, C.B.; ZECCm DE SOUZA, A. & CARDOSO DE ALMEIDA, P.C. – Tissue carcinoembryonic antigen in early breast carcinoma. Breast Cancer Res, Treat. 15: 213-216, 1990.
6. HEYDERMAN, F.; STEELE, K. & ORMEROD, M.G. - A new antigen on the epithelial membrane: its immunoperoxidase localization in normal and neoplastic tissue. J. clin, Path. 32: 35-39, 1979.
7. HSU. S.M.; RAINE, L & FANGER, H. - Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ARC and unlabelled antibody (PAP) procedures. J. Histochem, Cytochem.29: 577-580, 1981.
8. HURUMANN, J.; UCHAA, M. & OZZELLO, L-Io vitro synthesis of immunoglobulins and .other proteins by dysplastic and neoplastic human mammary tissues. Cancer Res. 36: 1284-1292, 1976.
9. KUHADJA, F.J.; OFFUTT, LE. & MENDELSOHN, G. - The distribution of carcinoembryonic antigen in breast carcinoma. Cancer, 52: 1257-1264, 1978.
10. MASON, D.Y. & TAYLOR, C.R. - Distribution of transferrin, ferritin and lactoferrin in human tissue. J. clin Path., 31: 316-327, 1978.
11. ORMEROD, M.G.; BUSSOLATI, G.; SLOANE, J.P.; STEELE, K. & GUGUOTTA, P. - Similarities of antisera to casein and epithelial membrane antigen. Virchows Arch. (AJ,397: 327-333, 1982.
12. PICH. A.; BUSSOLA TI, G. & CARBONARA, A. - Immunocytochemical detection of casein and casein-líke proteins in human tissues. J. Hístochern. Cytochem. 24: 940-944, 1976.
13. RUSSO, J. & RUSSO, I.M. - Immunocytochemical markers in breast cancer. In: De LELUS R. eds. Advances in Immunohistochemistry. New York, Raven, 1988. 431-475.
14. SHOUSHA, S.; LYSSIOTIS, T., GODFREY, V.M. & SCHEVER, P.J. - Carcinoembryonic antigen in breast cancer ti~sue: a useful prognostic indicator. Brit, Med. J. 1. 777-779, 1978.
15. VAN DER LINDEN, r.c, BAAK, J.P.; LlNDEMAN, J.; SMEULDERS, A.W.M. & MEYER. C.J.L.- Carcinoembryonic antigen expression and peanut agglutinin binding in clinical, histopathological, biochemical and morphometric features. Histopathology,9: 1051-1059, 1985.
16. W ALKER, R.A. - The demonstration of alphalactoalbumin in hurnan breast carcinomas. J. Palh.,129: 37-42, 1979.
17. WALKER, R.A. - Demonstration of carcinoembryonic antigen in human breast carcinomas by the immunoperoxidase technique. J. clin, Path., 33: 356-360, 1980.
18. WALKER, R.A. - Differention of human breast carcinomas: an imrnunohistological study of appropriate and inappropriate protein production. J. Palh.,137: 109-117, 1982.
19. WALKER, R.A. - Immunohistochemistry of biological markers of breast carcinoma. 10: De LELUS R. Advances in immunohistochemistry, New York, Masson Pub., 1984, p. 223-241.
20. WOODS, R.L; SEARLE, R.; WILSON, R.; BHAGSHA W, K. D. & COOPER, E.H. - Serum kappacasein in breast cancer. Eur. J. Cancer., 13: 1113- 1117, 1970
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