Abstract
The aim of the present study was to evalute the influence of perchloric acid concentration and temperature at the precipitation step on the determination of mucoproteins. Our results showed that the increase of perchloric acid concentration as well as of the temperature produce underestimated values of serum mucoproteins. Duto the clinical significance of the mucoproteins dosage for the accompaniment of patients with infectious diseases and metabolic disturbances, it is required a detailed control of externa! factors which can interfere on its quantification.
References
1. BOGDAl1JKOW A, B.; BERNACKA, K. & DROZD, J. - Immunoelektrophorese der perchlorsaureloslichen serumpproteine. Clin, chim. Acta.13:221-7, 1966.
2. DOCK, W. & SNAPPER, I. - Advances in internal medicine . Chicago, Yr. Bk. Pub., 1955. v. 7, p.101.
3. LEDVINA, M. & COUFALOVA, S. - Adsorption of blood serum mucoproteins on filter paper. Clin, chim. Acta., 6:16-21,1961.
4. MORAWIECKA, B. & MEJBAUMKATZENELLENBOGEN, W. - The quantitative determination of human seromucoid fractions in paper electrophoresis. Clin. chim. Acla, 7:722-8, 1962.
5. PRICE, W.H.; MATANOSKI, G.M; MORRISON, D.; PREWER, A. & WAGNER, G. – The fractionation of seromucoids from human serum. Buli. Johns llopk. llosp.,108:227-41, 1961.
6. SPIRO, R.G. - Glycoproteins: their biochemistry, biology and role in human disease. New Engl, J. Med.,281:991-1001, 1969.
7. WINZLER, R.I. & SMYTH, I.M. - Studies on mucoprotcins of human plasma; plasma mucoprotein levels in cancer patients. J. clin. Invest., 27:617- 9, 1948.
8. WINZLER, R.I. - Detcrmination of serum glycoproteins. In: GUCK D. ed. - Methods of Biochemical Analysis. New York, Interscience, 1955. v. 2, p. 279.
9. WINZLER R.I. - Glycoproteins of plasma. In: WOLSTYENHOLME, G.E.W. & O"COI'NOR, M.- Ciba Foundation Symposium on lhe chemistry and biology of mucopol ysacharides, Boston, Churchill, 1958. p. 245-267.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 1990 Revista do Instituto Adolfo Lutz