Keywords
hepatitis B vírus
molecular hybridization
How to Cite
Abstract
The micromethod for detection of the hepatitis B virus DNA polymerase activity described by LIN et alii was modified, and a comparative study between this two techniques was performed. Besides, two different kinds of filter paper utilized in the reaction, the GF/C and the AP20, were compared. All the tests showd viable results, although the modified Lin's technique and the filter AP20 showed larger differentials.
References
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2. LIN, H.J.; WU, P.C.; LAI, C.L. & CHAK, W. – Micromethod for phosphonoformic inhibition assay of hepatitis B viral DNA polymerase. Clin. Chem.,30: 549-552, 1984.
3. PINHO, J.R.R.; FONSECA, L.E.P.; SONG Y.; MIYAMOTO, Y.; CARRILHO, F.J.; GRANATO, C.F.H. & SILVA, L.C. - Comparison of serum hepatitis B virus replication markers in chronic patients: studies on HBeAg/Anti-HBe system, viral DNA polymerase and HBV-DNA. Rev. Inst, Med. trop, São Paulo, 31: 328-335, 1989.
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Copyright (c) 1990 Revista do Instituto Adolfo Lutz