Abstract
Among virulence factors in bacteria investigated in the Seção de Bacteriologia of the Instituto Adolfo Lutz, the occurrence of immunoglobulin A proteases (IgA1 proteases) has been evaluated. These extracellular enzymes are produced by different mucosal pathogens, including the three major agents of bacterial meningitis: Neisseria meningitidis, Haemophilus influenzae and Streptococcus pneumoniae. IgA1 proteases were detected by immunoelectrophoresis in 28 of 39 strains studied, bacteria and yeasts. Except for 7 bacteria from collection, ali organisms were isolated from cerebrospinal f1uid. ln the three most frequent etiological agents of meningitis, IgA1 proteases were observed, as well as in four other bacteria, two of which from collection, and not cited in the literature as enzyme producers.
References
2. KILIAN, M.; MESTECKY, J. & SCHROHENLOHER, R.E. - Pathogenic species of the genus Haemophilus and Streptoeoceus pneumonia e produce immunoglobuline A1 protease. lnfeet. immunol., 26(1): 143-149, 1979.
3. KILIAN, M., THOMSEN, B., PETERSEN, T.E. & BLEEG, H. - Molecular biology of Haemophilus influenzae IgA1 proteases. MoI. Immunol., 20: 1051-1058, 1983.
4. KILIAN, M., THOMSEN, B., PETERSEN, T.E. & BLEEG, H. - Occurrence and nature of bacterial IgA proteases. Ann. New York Aead. Se., 409: 612624, 1983.
5. KILIAN, M.; REINHOLDT, J.; LOMHOLT, H; POULSEN, K. & FRANDSEN, E.Y.G. - Biological significance of IgA1 proteases in bacterial colonization and pathogenesis: critical evaluation of experimental evidence. APMlS, 104: 321-338, 1996.
6. INSEL, R.A.; ALLEN, P.Z & BERKOWITZ, I.D. - Types and frequency of Haemophlus influenzae IgA1 proteases. In: Seminars in infectious Diseases, voI. 4, Weinstein, L & Fields, B.N., eds., Grune & Straton, New York, 1982, p. 225.
7. LANDGRAF, I.M. & VIEIRA, M.FP. - Biotypes and serotypes of Haemophilus influenrae from patients with meningitis in the City of São Paulo, BraziI. J. clin. Mierobiol., 31: 743-745, 1993.
8. LOMHOLT, H. - Molecular biology and vaccine aspects of bacterial immunoglobulin A1 proteases. APMlS Suppl. 62, 1104: 5-28,1996.
9. MANSA, B. & KILIAN, M. - Retained antigen-binding activity of Fab fragments of human monoclonal immunoglobulin A1 (IgA 1) cleaved by IgA1 protease.lnfeet. Immunol. 52:171-174,1986.
10. MESTECKY, J. & KILIAN, M. - Immunoglobulin A (IgA) . Methods in Enrymology, 116:37-75, 1985.
11. MEHTA, S.K.; PLAUT, A.G.; CALVANICO, N.J. & TOMAS I, Jr, T.H. - Human immunoglobuline A: production of an Fc fragment by an enteric microbial proteolytic enzyme. J. Immunol. 111: 1274, 1973.
12. MULKS, M.H. - Microbial IgA proteases. ln: Microbial Enzymes and Virulence. Holder, I.A., ed., CRC Press, Boca Raton, Florida, 1985, p. 81-104.
13. PLAUT, A.G.; GENCO, R.J. & TOMASI, Jr., T.B. - Isolation of an enzyme from Streptococcus sanguis which specifically cleaves IgA. J. Immunol. 113: 289-291, 1974.
14. PLAUT, A.G. - Microbial IgA proteases. N. Engl. J. Med. 298: 1459-1463, 1978.
15. PLAUT, A.G. - The IgAl proteases of pathogenic bacteria. Ann. Rev. Mierobiol. 37: 603-622, 1983.
16. SKEA, D.L.; CHRISTOPOULOUS, P.; PLAUT, A.G. & UNDERDOWN, B.J. - Studies on the specificity of the IgA-binding lectin, Jacalin. MoI. Immunol. 25 (1): 1- 6, 1988.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 1998 Instituto Adolfo Lutz Journal