Keywords
Chelating Agent
Radiolabelling
How to Cite
Abstract
The epidermal growth factor (EGF) is a little polypeptide of 6kD MW capable of stimulation the proliferation of epithelial cells and performs this biological activity through a membrane receptor, a 1186 aminoacid glycoprotein of 170 kD MW. This receptor (EGF- R) in high levels have been detected in malignant tumours of epithelial origin, i.e., breast, ovary, vulva, esophagus, colon and lung tumours.
The anti EGF receptor monoclonal antibody, ior-egf/r3 (Cuba) is an IgG2a isotype was labeling with 99mTc. The reduction step was performed with 2-mercaptoethanol at a molar ratio of 1:1000 (2ME:MAb) The integrity of reduced MAb was checked by gel filtration chromatography on Sephadex G-25 minicolumn (1 X 10 em). The MAb pyke was submitted to two types of methylen diphosphonate as chelant:Amerscam Medronate II Bone Agent MDP kit (Amersham, UK) and IPEN/CNEN MDP kit. and both were labeled with 1 mCi (37 MBq) of 99m Te eluted from 99Mo/99mTc generator system ( IPEN/CNEN-SP). The purification in ITLC reveals the high grade of labeling monoclonal antibody. The laboratory tests did not show significant modification in structure of MAb - 99mTc macromolecule in both cases and can be used in immunoscintigraphy.
References
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