Immunodiagnosis of human leptospirosis. 1. Polysaccharide antigen for passive hemagglutination test
Vol. 41 No. 2 (1981), ORIGINAL ARTICLE
Vol. 41 No. 2 (1981)

Immunodiagnosis of human leptospirosis. 1. Polysaccharide antigen for passive hemagglutination test

ORIGINAL ARTICLE
https://doi.org/10.53393/rial.1981.41.36769
Published 1981-12-30
Maricy Alves Ribeiro
Instituto Adolfo Lutz
Massami Kawarabayashi
Instituto Adolfo Lutz
Augusta Kiyomi Takeda
Instituto Adolfo Lutz
pdf (Português (Brasil))

Keywords

leptospirosis
human
immunodiagnosis
antigens
polysaccharidie
passive hemagglutination test

How to Cite

1.
Ribeiro MA, Kawarabayashi M, Takeda AK. Immunodiagnosis of human leptospirosis. 1. Polysaccharide antigen for passive hemagglutination test. Rev Inst Adolfo Lutz [Internet]. 1981 Dec. 30 [cited 2024 Jul. 3];41(2):127-33. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/36769
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Abstract

Antígenos polissacarídicos F. foram extraídos de leptospiras dos sorotipos icterohaemorrhagiae e pato c, cuja relação proteína/carboidrato foi de 1,24 e 0,54, respectivamente. Sua especificidade sorológica foi avaliada na reação de hemaglutinação passiva para o diagnóstico da leptospirose humana. Para tanto, hemácias de carneiro previamente fixadas com glutaraldeído foram sensibilizadas
com ambas as preparações. Esta prova foi experimentada em 91 soros obtidos de 35 pacientes com leptospirose, causada presumivelmente pelos sorotipos icterohae-morrhagiae, copenhageni, grippothyphosa e pomona. Verificou-se que ambas as frações F. comportaram-se de maneira semelhante. A reação de hemaglutinação passiva foi gênero específica, bastante sensível, positivando-se precocemente na fase aguda da doença. Foram salientadas as vantagens do emprego do antígeno F. patoc, por se tratar de sorotipo não patogêncio.

https://doi.org/10.53393/rial.1981.41.36769
pdf (Português (Brasil))

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