Abstract
Continuous-line cultures of hamster kidney cells (BHK-21) was found to be a more rapid and sensitive procedure for isolation of mumps virus as compared with the embryonated chicken egg procedure. BHK-21 cells can be easily maintained in the virus labotory, thus contrasting with similarly sensitive primary cells of monkey kidney wh ich are difficult to obtain and cannot be maintained. Use of BHK-21 cell cultures for the isolation of mumps virus seems to be a useful alterna tive procedure.
References
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2. COONEY, M.K.; FOX, J.P. & HALL, C.E. The seattle vírus watch. VI. Observations
of infections with and illness due to parainfluenza, mumps and respiratory syncytial viruses and mycoplasma pneumoniae. Amer. J. Epidemiol., 101: 532-51,
1975.
3. HENLE, G.; HENLE, W.; WENDELL, K.K. & ROSENBERG, P. Isola tion of mumps virus from human beings with induced apparent or inapparent infections.
J. exp. Med., 88:223-32, 1948.
4. HENLE, G. & DEINHARDT, F. - Propagation and primary isolation of mumps virus in tissue culture. Proc, Soc. exp. Biol. Med., 89 :556-60, 1955.
5. HOOPS, H.E. & PARKMAN, P.D. - Mumps virus. In: LENNETTE, E.H. &
SCHMIDT, H.J. - Diagnostic procedure for viral, rickettsial and chlamydial in-
fections. 5th ed. Washington, A.P.H.A., c1979, p. 633-53.
6. KILHAM, L. - Mumps meningoencephalitis with and without parotitis. Amer. J. Die. Child., 78 :324-33, 1949.
7. LENNETTE, E.H.; JENSEN, F.W.; GUENTHER, R.W. & MAGOFFIN, R.L. - Serologic responses to para-influenza viruses in patients with mumps virus infection. J. Lab. clin. Med., 61: 780-88, 1963.
8. UTZ, J.P.; KASEL, J.A.; CRAMBLETT, H.G.; SZWED, C.F. & PARROTT, R.H.
Clinical and laboratory studies of mumps. I. Laboratory diagnosis by tissue-culture
technics. New. Engl. J. Med., 257:497-502, 1957.
9. WELLER, T.H. & ENDERS, J.F. - Production of hemmagglutinin by mumps and influenza A viruses in suspended cell tissue culture. Proc. Soc. exp. Biol. Med., 69: 124-8, 1948.
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Copyright (c) 1983 Instituto Adolfo Lutz Journal
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