Keywords
Corynebacterium diphtheriae, isolation from human blood
blood, isolatíon of C. diphtheriae
blood, isolatíon of C. diphtheriae
How to Cite
1.
Sacchi CT, Tondella MLC, Brandileone MC de C, Melles CEA, Paula MDN de. Isolation of Corynebacterium diphtheriae from human blood. Rev Inst Adolfo Lutz [Internet]. 1985 Dec. 30 [cited 2024 May 13];45(1-2):73-9. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/36853
Abstract
C. diphtheriae was isolated and identified from 3 blood specimens
from a patient without clinical manifestation of diphtheriae. Morphology and bíoehemical and toxigenic properties of the three isolates corresponded to biotypes intermedius, mitis, and mitis variety belfanti. The biochemical properties were identical for the three isola tes, except for type A nitrate-redutase in biotypes intermedius and mitis. These biotypes were not toxigenic when tested by Elek method. Biotype mitis variety belfanti was toxigeníc, There was no difference in the behavior of isolates against the tested anti-microbial agents.
References
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bility testing by a standardized single disk method. Amer. J. clin. Pathol., 45:493-6, 1966.
2. BEZJAK, V. - Differentiation of C. diphtheriae of the mitis type found in díphthría and Ozaena. Antonie van Leeuurenhder, Amsterdam, 20 :269-271, 1954.
3. BEZJAK, V. & FARSEY, S.J. - C. diphtheriae in skin lesions in Ugandan children.
Bull. WHO, 43:643-50, 1970.
4. BRANDILEONE, M.C.C.; MELLES, C.E.A.; VAZ, T.M.L; NEME, S.M. & PESSOA, G.V.A. - Considerações sobre os resultados de 5.860 casos de hemoculturas realizadas em pacientes do município de São Paulo, entre 1979 e 1982. Rev. Inst, Adolfo Lutz, 44: - ,1984.
5. BROOKS, R. - Guidelines for the laboratory diagnosis of diphtheriae. Geneva, WHO (1981]. 27 p. (LAB/8l. 7).
6. CARPENTER, C.M.; SUHRLAND, L.G. & MORRISON, M. - The oxalate salt of p-aminodimetlrylaniline, an improved reagent for the oxidase testo Seience, Wash., DC, 105:649, 1947.
7. CHANG, N.D.; LAUGHREN, G.S. & CHALVARDJIAN, N.E. - Three variants of C. diphtheriae subsp. mitis (belfan ti) ísolated from a throat specimen. J. clin. Microbiol., 8: (6)767-8, 1978.
8. COWAN, S.T. & STEEL, K.J. - Manual for the identification of medical bacteria. 2nd ed. Cambridge, Cambridge University press, 1975. p. 167.
9. DIFCO LABORATORIES - Difco manual: medios de cultivo deebidratadoe y reactio118 para microbiologia. 10.a ed. Detroit, Mich., Difco, 1984. p. 263.
10. HEWITT, L.F. - Haemolytic activity of C. diphtheriae. J. Pathol, Bacteriol., 59:
145-57, 1947.
11. INSTITUT PASTEUR - Milieux et réactifs de Laboraioire Paeteur. Paris, Inst. Pasteur, 1978. p. 83, 116, 186.
12. KING, E.O.; FROBISHER, M. & PARSONS, E.I. - The in vitro test for virulence of
C. diphtheriae. Amer. J. Public Health; 39: 1314-20, 1949.
13. MAC FADDIN, J.F. - Biochimical test« for identification o] medical bacteria. 2nd ed. Baltimore, Wíllians & Wilkins, 1980, p. 51-58.
14. McLEOD, J.W. - 'I'lre types mitis intermedius and gravis of C. diphtheriae, Bacteriol, ne«, 7: 1-41, 1943.
15. MO ORE, M.S. & PARSONS, E.I. - A study of a modífied Tinsdale's medíum for the primary isolation of C. diphtheriae. J. infect. tu«, 102 :88-93, 1958.
16. PICHINOTY, F. & PIECHAUD, M. - Recherche des nitrate-reductase A et B:
méthodes, Ann. Inst: Pasteur; Paris, 114: 77-98, 1968.
17, REID, J. & GREENWOOD, L. - Corynebacterial endocarditis. Arch. interno Med., 119:10€-10, 1967.
18, RILEY, P.S.; HOLLIS, D.G.; UTTER, G.B.; WEAVER, R.E. & BAKER, C.N. -
Characterization and identification of 95 diphtheroid (Group JK) cultures isolated from elinícal specimens. J. Clin. Microbiol., 9:418-424, 1979.
19, SANDLER, M. - C. diphtheriae endoearditis in an adult with congenital cyanotic Jreart disease, S. af r, medo J., 61 :594, 1982.
20, SARAGEA, A.; MAXIMESCU, P. & MEITERT, E. - Corynebacterium diphtheriae:
microbiological methods used in clinícal and epidemiological investigations. In:
BERGAN, T. & NORRIS, J.R. - Methods in microbiology. London, Academic press, 1979. V. 13, p. 61-176.
21. SIERRA, G. apud COWAN, s.r. & STEEL, R.J.8. p. 167.
22. SONNENWIRTH, A.C. - Bacteriemia: alcance de problema. In: Sonnenwirth, A.C. - Baeteremia : aepectos clinic08 y de laboratorio, Trad. do inglês por Editorial Médica Panamericana. Mexico, Panamericana, 1975. p. 15-28.
23, SOTTNEK, F.O. & MILLER, J.M. - Ieolation. and identilication 01 C. diphtheriae. Atlanta, Center for Disease Control, 1980 (revised 1982). 14 p.
24, WILDFUHR, G. - Zur Frage der Bakteriamie in Beginn der Diptherie. Zentralbl. Bakteriol. I Abt Orig. A, 54:14-7, 1949.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 1985 Instituto Adolfo Lutz Journal
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