Keywords
DL-lysine in dietetic supplements and pharmaceuticals, determination
dietetic supplements, DL-lysine in, determination
pharmaceuticals, DL-lysine in, determination
dietetic supplements, DL-lysine in, determination
pharmaceuticals, DL-lysine in, determination
How to Cite
1.
Chaves MA, Akatuka AS, Auricchio MT. Determination of DL-lysine in pharmaceuticals and dietetics products. Rev Inst Adolfo Lutz [Internet]. 2021 Aug. 13 [cited 2024 May 18];48(1-2):49-55. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/36906
Abstract
A method for quantification of DL-lysine monochloride in pharmaceuticals and dietetics products without previous extraction was proposed. Other substances as buclizine hydrochloride, vitamin A, D and Bcomplex, tryptophan, glutamic acid, betaine hydrochloride, when presented in such products, did not interfere in this reaction. In this method, lysine reacts with sodium tetraborate and sodium nitroprussiate, producing a colored compound, which exhibits maximum absorption at the wavelength at about 545nm.
References
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12. TILLER, 1.M. & BLOXAM, D.L. - An enzymatic fluorometric assay for L-Jysine in plasma and tissue. Anal. Biochem., 131:426-9, 1983.
13. TOMARELLI, R.M.; YUHAS, R.l.; FISHER, A. & WEABER, l.R. - An HPLC method for the determination of reactive (avaiable) lysine in milk and infant formulas. J, Agric. Food Chem., 33:316-8,
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14. VIENOZINSKIENE, J.; JANUSEVICIUTE, R.; PAULIUKONIS, A. & KAZLAUSKAS, D. Lyssine decarboxylase assay by lhe pH -stat
method. Anal. Biochem., 146:180-3, 1985.
15. ZITTLE, C.A. & ELDRED, N.R. - Determination of L-lysine with a specific decarboxylase. J. Biol. Chem., 156:401-9, 1944.
2. DAVIS, A.T., INGALLS, S.T. & HOPPEL, eL.Determination of free trimethyllysine in plasma and tissue specimens by high-performance liquid chromatography. r. Chrotnat ogr., 306:79-87,
1984.
3. FINLEY, 1.W., & FRIEDMAN, M. - Chemical methods for avaiable lysine. Cereal Chem., 50:101-5,1973.
4. GREEMSTEIN, 1.P. & WINITZ, M. - Chemistry of lhe amino acids. New York, John Wiley, 1961. v.3, p.2097-124.
5. HENNECKE, M. & PLAPP, B.V. - Quantitative analysis of lysine anaJogs and derivatives. Anal. Biochem., 136:110-18,1984.
6. KAKADE, M.L. & LIENER, E.1. - Determination of avaiabJe lysine in proteins. Anal. Biochem., 27:273-80, 1969.
7. KELLEY, J. & CHRIN, L. - Thin-layer chromatography of collagen analysis. 1. Chromatogr., 311:400-4, 1984.
8. MORO, L.; MODRICKY, C.; STAGNI, N.; VITTOR, F. & de BERNARD, B. - High-performance liquid chromatographic anaJysis of urinary
hidroxylysyl glicosides as indicator of collagen turnover. Analyst, 109:1621-2, 1984.
9. NEUBERGER, A. & SANGER, F. - The metabolism of lysine. Biochem. l., 38:119-25,1944.
10. NEUBERGER, A. - The lysine content of casein and zein. Biochem. l., 158:717, 1945.
11. PHARMACOPÉE française, 8ellle ed. Paris, Ordre National des Pharmaciens, 1965. p.653-4.
12. TILLER, 1.M. & BLOXAM, D.L. - An enzymatic fluorometric assay for L-Jysine in plasma and tissue. Anal. Biochem., 131:426-9, 1983.
13. TOMARELLI, R.M.; YUHAS, R.l.; FISHER, A. & WEABER, l.R. - An HPLC method for the determination of reactive (avaiable) lysine in milk and infant formulas. J, Agric. Food Chem., 33:316-8,
1985.
14. VIENOZINSKIENE, J.; JANUSEVICIUTE, R.; PAULIUKONIS, A. & KAZLAUSKAS, D. Lyssine decarboxylase assay by lhe pH -stat
method. Anal. Biochem., 146:180-3, 1985.
15. ZITTLE, C.A. & ELDRED, N.R. - Determination of L-lysine with a specific decarboxylase. J. Biol. Chem., 156:401-9, 1944.
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