Keywords
garlic (Allium sativum), hidroalcoholic extract of, identification
garlic, essential oil of, identification
pharmaceuticals, garlic hidroalcoholic extract in, detection
pharmaceuticals, garlic essential oil in, detection
oils, garlic essential oil detection
thinlayer chromatograph
garlic, essential oil of, identification
pharmaceuticals, garlic hidroalcoholic extract in, detection
pharmaceuticals, garlic essential oil in, detection
oils, garlic essential oil detection
thinlayer chromatograph
How to Cite
1.
Hoppen VR, Auricchio MT, Batistic MA. Thin-layer chromatography of preparations from Allium sativum L. Rev Inst Adolfo Lutz [Internet]. 1989 Jun. 30 [cited 2024 May 19];49(1):5-10. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/36923
Abstract
By using thin-Iayer chromatography the detection of characteristic substances contained in garlic such as the compounds responsible by the scent and those of aminoacid nature were investigated. Such study was made by analyzing hidroaIcoholic extracts and preparations containing histologic elements of Allium sativum L. Two thin-Iayer chromatographic systems were selected in order to detect these two groups of compounds in preparations containing garlic oil or garlic hydroalcoholic extract.
References
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2. BRODNITZ, M.H.; PASCALE, J.V. &DERSLICE, L.V. - Flavor components of garlic extract. J. agric.FoodChem., 19(2): 273-5, 1971.
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11. GUENTHER, E. - The essentialoils. 1. Mitteilung: chromatographishce Untersuchungen über die genuinen Inhaltsstoffe - von Allium sativum L. New York, Van Nostrand, 1952. p. 67-9.
12. HaRHAMMER, L.; WAGNER, H.; SEITZ, M. & VEJDELEK, Z.J. - Zur Wertbestimmung von Knoblauchprãparaten, Pharmazie, 23: 462-6, 1968.
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18. SCHULTZ, O.E. & MOHRMANN, H.L. - Beitrag zur Analyse der Inhaltsstoffe von Knoblauch Allium sativum L. 2. Mitteilung: gaschromatographie des Knoblauchõls, Pharmazie, 20(7):441- 7, 1965.
19. STAHL, E., ed. - Thin-IaXâr chromatography: a laboratory handbook. 2 ed. English translation by M.R.F. Achworth. Berlin, Springer- Verlag, 1969. p. 1041.
20. STOLL, A. & SEEBECK, E. - Chemical investigations on Alliin, the specific principIe of garlic. Adv. Enzymol., 11:377-400, 1951.
21. STOLL, A. & SEEBECK, E. - Über Alliin, die genuine Muttersubstanz des Knoblauchõls, 1. Mitteilung: Über chromatographie. Helv. Chim, Acta, 31: 189-210, 1948.
22. VOIGT, M. & WOLF, E. - Knoblauch-HPLC Bestimmung von Knoblauchwirkstoffen in Extrakten, Pulver und Fertigarzneimitteln. Dtsch. Apoth. Ztg., 126 (12): 591-3, 1986.
2. BRODNITZ, M.H.; PASCALE, J.V. &DERSLICE, L.V. - Flavor components of garlic extract. J. agric.FoodChem., 19(2): 273-5, 1971.
3. CARSON, J.F. & WONG, F.F. - A new colorimetric test for detecting sulfur-containing amino acids. J. Chromatogr., 12 (1903): 408-9, 1963.
4. CA VALLITO, C.J. & BAILEY, J .H. - Allicin, the antibacterial principIe of Allium sativum. r. Isolation, physical properties and antibacterial action. J. am. chem. Soc., 66: 1950-1,1944.
5. CA VALLITO, C.J.; BAILEY, J.H. & BUCK, J.S.The antibacterial principIe of Allium sativum. III. Its precursor and "essential oil of garlic". J. amo chem. Soc., 67: 1032-3, 1945.
6. CAVALLITO, C.J., BUCK, J.S. & SUTER, C.M.Allicin, the antibacterial principIe of Allium sativum, II. Determination of the chemical structure. J. amo chem, Soc., 66: 1952-4, 1944.
7. COSTA, A.F. -Fannacognosia. 2~ ed.Lisboa, Fundação Calouste Gulbenkian [1967]. v. 2, p. 925-7.
8. COSTA, A.F.-Fannacognosia. v. 3: Farmacognosia experimental. 2~ ed. Lisboa, Fundação Calouste Gulbenkian [1967]. p. 482-5.
9. ESSMAN, E.J. - The medicinal uses of herbs. Fitoterapia, 55 (5): 279-89, 1984.
10. GRANROTH, B. - Separation of Allium sulfur amino acids and peptides by thin-Iayer electrophoresis and thin-Iayer chromatography. Acta chem Scand., 22 (10): 3333-5,1968.
11. GUENTHER, E. - The essentialoils. 1. Mitteilung: chromatographishce Untersuchungen über die genuinen Inhaltsstoffe - von Allium sativum L. New York, Van Nostrand, 1952. p. 67-9.
12. HaRHAMMER, L.; WAGNER, H.; SEITZ, M. & VEJDELEK, Z.J. - Zur Wertbestimmung von Knoblauchprãparaten, Pharmazie, 23: 462-6, 1968.
13. JÁGER, H. - Quantitative Bestimmung von Allicin in Frischen Knoblauch. Arch. Pharm., Weinhein, 288 (3): 145-8,1955.
14. MARTlNDALE, W.H. - The extra pharmacopoeia; edited by L.F. Reynolds and Anne B. Prasad. 28 th ed. London, Pharmaceutical Press, 1982. p.688-9.
15. MICHAHELLES, E. apud MIETHING, H. 16.
16. MIETHING, H. - Allicin und ói in Knoblauchzwiebeln - HPLC - Gehaltsbestimmung. Dtsch. Apoth. Ztg., 125 (41): 2049 - 50,1985.
17. SCHULZ, O.E. & MOHRMANN, H.L. - Beitrag zur Analyse der Inhaltsstoffe von Knoblauch - Allium sativum L. I. Mitteilung: Dumnschichtchromatographie des Knoblauchõls. Pharmazie, 20 (7): 379-81,1965.
18. SCHULTZ, O.E. & MOHRMANN, H.L. - Beitrag zur Analyse der Inhaltsstoffe von Knoblauch Allium sativum L. 2. Mitteilung: gaschromatographie des Knoblauchõls, Pharmazie, 20(7):441- 7, 1965.
19. STAHL, E., ed. - Thin-IaXâr chromatography: a laboratory handbook. 2 ed. English translation by M.R.F. Achworth. Berlin, Springer- Verlag, 1969. p. 1041.
20. STOLL, A. & SEEBECK, E. - Chemical investigations on Alliin, the specific principIe of garlic. Adv. Enzymol., 11:377-400, 1951.
21. STOLL, A. & SEEBECK, E. - Über Alliin, die genuine Muttersubstanz des Knoblauchõls, 1. Mitteilung: Über chromatographie. Helv. Chim, Acta, 31: 189-210, 1948.
22. VOIGT, M. & WOLF, E. - Knoblauch-HPLC Bestimmung von Knoblauchwirkstoffen in Extrakten, Pulver und Fertigarzneimitteln. Dtsch. Apoth. Ztg., 126 (12): 591-3, 1986.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 1989 Instituto Adolfo Lutz Journal
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