Immunological specificity of polysaccharides obtained from different groups of Neisseria meningitidis
Vol. 34 No. 1-2 (1974), ORIGINAL ARTICLE
Vol. 34 No. 1-2 (1974)

Immunological specificity of polysaccharides obtained from different groups of Neisseria meningitidis

ORIGINAL ARTICLE
https://doi.org/10.53393/rial.1974.34.37065
Published 1974-12-30
Solange Barros Carbonare
Instituto Adolfo Lutz
Augusta Kiyomi Takeda
Instituto Adolfo Lutz
Filomena B. M. Jordão
Instituto Adolfo Lutz
Augusto de E. Taunay
Instituto Adolfo Lutz
pdf (Português (Brasil))

Keywords

Neisseria meningitidis
polysaccharides from Neisseria meningitidis, immunological specificity

How to Cite

1.
Carbonare SB, Takeda AK, Jordão FBM, Taunay A de E. Immunological specificity of polysaccharides obtained from different groups of Neisseria meningitidis. Rev Inst Adolfo Lutz [Internet]. 1974 Dec. 30 [cited 2024 Dec. 26];34(1-2):119-25. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/37065
ACM ACS APA ABNT Chicago Harvard IEEE MLA Turabian Vancouver

Download Citation

Endnote/Zotero/Mendeley (RIS) BibTeX

Abstract

Polysaccharides obtained from eight Neisseria meningitidis serogroups were studied with respect to their immunological specificity. Antigen was obtained from Iíquíd medium cultures by means of precipitation made with Cetavlon (cetyl-triemethyl ammonium brorníde). The immunological specificity of such polysaccharídes was studies by means of counterimmunoelectrophoresis and haemagglutination assays.
The sera employed in thís assays was obtained from rabbits immunized with Neisseria meningitidis of A, B, C, D, 29E, X, Y and Z groups. No cross-reactíons were observed among any of the polysaccharides here obtained.

https://doi.org/10.53393/rial.1974.34.37065
pdf (Português (Brasil))

References

1. GOTSCHLICH, E. C.; LIU, T. Y. & ARTENSTEIN, M. S. - Human ímmuntty to the meningococcus. lU. Preparation and ímmunocnemíca; properties of the group A. J. exp. Med., 129: 1349-65, 1969.

2. LOWRY, O. H.; ROSEBROUGH, N. J.; FARR, A. L. & RANDALL, R. J. - Protein measurement with the Folin phenol reagent. J. biol. Chem., 193: 265-75, 1951.

3. PALHARES, M.; GELLI, S. S.; ALMEIDA, M. C. R.; MELLES, C. E. A.; TAKEDA, A. K. & TAUNAY. A. E. - Pesquisa de polissacarídeos de Neisseria meningitidis do grupo C no líquido cefalorraquidiano por imunoeletroforese cruzada em acetato de celulose. Rev. Inst. Adolfo Lutz, 33: 85-9, 1973.

4. SCOTT, T. A. & MELVIN, E. H. - Determination of dextran with anthrone. Analyt. Chem., 25: 1656-61, 1953.

5. SVENNERHOLD, L. - Quantitative estimation of sialic acids. n. A colorimetric resorcinol-hydrochloric acid method. Biochem. biophys. Acta, 24: 604-611, 1957.

6. ZOELLNER, N. & KIRSCH, K. - Uber die quantitative Bestimmung von Lipoiden (Mikromethode) mitteis der víelen nat'urlíschen Lipoiden (allen beanten Plasmalipoden) gemeinsamen sulfophospho-vanllin Reaktion. Z. ges. exp. Med., 135: 545, 1962.

7. TAKEDA, A. K.; TAUNAY, A. E.; SCALABRINI, L. G. P. & CASTRO, 1.0. - Arrtícorpos antipolissacarídeo C de Neiseria meningitidis: detecção através da hemaglutinação passiva em soros de pacientes e de vacinados. Rev. Inst. Adolfo Lutz, 34: 127-133, 1974.
Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright (c) 1974 Instituto Adolfo Lutz Journal

Downloads

Download data is not yet available.