Keywords
cerebrospinal fluid
total protein, methods for determination
total protein, methods for determination
How to Cite
1.
Vermes LMS, Ferri RG, Marlet JM. Comparative study of methods for determining total proteins (in cerebrospinal fluid). Rev Inst Adolfo Lutz [Internet]. 1977 Dec. 30 [cited 2024 Jul. 3];37(1-2):11-6. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/37093
Abstract
Four procedures for determining total protein in diluted biological fluids (specifically cerebrospinal fluid) were compared as to accuracy, precision, sensibility and fluid consumption, The methods investigated were: trichloroacetic acid turbidimetric method, colorimetry of the protein solutions previously treated
with alkaline-copper and Folin-Ciocalteu reagents (Lowry's method), the sulphosalicylic
acid turbidimetric method, and the procedure devised by Heer & Margni in which colorimetry is done on the eluate of the protein solution previously applied to a cellulose acetate strip and dyed. In an overall view, Lowry's method proved
to be the most suitable for routine determinations of total proteins in diluted biological fluids because of its higher accuracy, sensibility and lower amount of Iiquid required.
References
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im Liquor. Klin W8chr., 30: 277-9, 1952.
2. BOSSAK, H.N., ROSENBERG, A.A. & HARRIS, A. - A quantitative turbidimetric method for the determination of spínal fluid protein. J. venero Dis, Imf orm, 30: 103-3, 1949.
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4. CARTIER, P. & PICARD, J. - Étude comparée de quel ques méthodes récentes de dosages des protéines dans les liquides biologiques. Ann. Biol. clin., 15: 459-68, 1957.
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Eiweissbestimmung im Liquor
und eiweissarmen LOsungen. Klin. Wschr, 33: 879-84, 1955.
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7. GORNALL, A.G.; BARDAWILL, C.J. & DAVID, M. M. - Determination of
serum proteins by means of the biuret reaction. J. biol. Chem., 177: 751-66, 1949.
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10. HENRY, R.J.; SOBEL, C. & SEGALOVE, M. - Turbidimetric determination of proteins with sulfosalicylic and trichloroacetic
acids. Proc. Soc. exp, Biol., 92:
748-51, 1956.
11. KIRK, P.L. - The chemical determination oí proteins. Advanc. Protein Chem., 3: 139-67, 1947.
12. KLUGE, H. von; WINKLER, G. & WIECZOREK, V. - Ergbnisse aus Vcrgleichen verschiedener Methoden zur Bestimmung
des Gesamtproteins in Liquor
cerebrospinalis. Dtsch. Gesundh.- Wes., 23: 2039-41, 1968.
13. LOWRY, O.H.; ROS0EBROUGH, N. J. ; :FARR, A.L.& RANDALL, R.J. - Protein measurement with the Folin phenol reagen t. J. biol. Chem., 193: 265-75, 1951.
14. MEULEMANS, O. - Determination of total protein in spinal fluid with sulphosalicylic
acid and trichloroacetic acid.
Clin. chim. Acta, 5: 757-61, 1960.
15. PASHLEY, D. H.; SCHUSTER, G. S.; P ALMER, P. & SHARAWY, M.M. - Some drugs and other substances that interfere wíth protein determination. Clin. Chem., 19: 263-65, 1973.
16, PLUM, C.M.; HERMANSEN, L. & PETERSEN, I. - Fractionated protein
determination on small quantities
(C.S.F., urine, serum dilution). Scand. J. clin. Lab. Invest., 7 (Suppl. 18): 3-35, 1955.
17. RICE, E. W . - Total proteins in cerebrospinal fluid CTurbimetric). Stand. Meth. clin. Chem., 2: 231-36, 1965.
18. RICE, E. W. & LOFTIS, J. W. - Critiqueof the determina tion of proteins in cerebrospinal
fluido Evaluation of the biuret method of Goa and the TCA turbidimetric method of Meulemans. Clin. Chem., 8: 56-61, 1962.
19. RIEDER, H. P. - Vergleich einiger Methoden zur Bestimmung des Gesamteiweisses
im Liquor und anderen stark verdünten Lõsungen. Clin. chim. Acta, 3: 455-70, 1958.
20. RIEDER, H. P. - Eine neue Modifikation der Cu-Folin-Methode zur Bestimmung des Total-proteins im Liquor cerebrospinalís, Klin. Wschr., 44: 1036-40, 1966.
21. SETHNA, S. & TSAO, M.U. - Protein level in cerebrospinal fluido Clin. Chem, 3: 249-56, 1957.
22. SPINA-FRANÇA, A. & AMAR, I. - Valoresnormais da concentração protéica do líquido cefalorraquidiano: variações ligadas ao local de colheita da amostra.Arq. Neuro-psiquiat. (S. Paulo). 19: 220-25, 1961.
23. SPINA-FRANÇA, A.; CERQUE IRA, F.M.C. & AMAR, I. - Proteinorraquia. Estudo crítico de métodos de dosagem. Arq. N europsiquiat. (São Paulo), 13: 303-12, 1955.
24. SVENSMARK, O. - Determination of protein in cerebrospinal fluido A comment
on the Lowry method. Scand. J. clin. Lab. Invest., 10: 50-2, 1958.
25. VERMES, L.M.S. - Proteínas totais do líquido cefalorraqueano: métodos de determinação e níveis nonnais (variações ligadas ao sexo, idade e local de punção).
São Paulo, 1975. [Tese - Instituto
de Ciências Biomédicas da Universidade de São Paulo]
26. ZONDAG, H.A. & BOETZELAER, G.L. - Determination of protein in cerebrospinal fluido Sources of error in the Lowry rnethod. Clin. chim. Acta, 5: 155-6, 1960.
im Liquor. Klin W8chr., 30: 277-9, 1952.
2. BOSSAK, H.N., ROSENBERG, A.A. & HARRIS, A. - A quantitative turbidimetric method for the determination of spínal fluid protein. J. venero Dis, Imf orm, 30: 103-3, 1949.
3. BURTIN, P.; DOUTRIAUX, D. & POCIDALO, J.J. - Contribution a l'étude de la protéinorachie. Intérêt de la mêthode du biuret. Presse méd., 66: 413-6 1958.
4. CARTIER, P. & PICARD, J. - Étude comparée de quel ques méthodes récentes de dosages des protéines dans les liquides biologiques. Ann. Biol. clin., 15: 459-68, 1957.
5. EGGSTEIN, M. & KREUTZ, F.H. - Vergleichende Untersuchungen zur quantitativen
Eiweissbestimmung im Liquor
und eiweissarmen LOsungen. Klin. Wschr, 33: 879-84, 1955.
6. GOA, J. - Microbiuret method for protein determination. Determination of total protein in cerebrospinaI fluid. Scand. J. clin. Lab. Invest., 5: 218-22, 1953.
7. GORNALL, A.G.; BARDAWILL, C.J. & DAVID, M. M. - Determination of
serum proteins by means of the biuret reaction. J. biol. Chem., 177: 751-66, 1949.
8. HEER, E.E. & MARGNI, R.A. - Electro e inmunoelectroforesis: manual de laboratorio e interpretaciones fundamentaleso
Buenos Aires, Gumersindo F. Fernandez, 1971. p. 269-76.
9. HENRY, R.J. - Clinical chemistry: principies and technics. 3rd. ed. New York, Harper & Row, 1965. p. 173-253.
10. HENRY, R.J.; SOBEL, C. & SEGALOVE, M. - Turbidimetric determination of proteins with sulfosalicylic and trichloroacetic
acids. Proc. Soc. exp, Biol., 92:
748-51, 1956.
11. KIRK, P.L. - The chemical determination oí proteins. Advanc. Protein Chem., 3: 139-67, 1947.
12. KLUGE, H. von; WINKLER, G. & WIECZOREK, V. - Ergbnisse aus Vcrgleichen verschiedener Methoden zur Bestimmung
des Gesamtproteins in Liquor
cerebrospinalis. Dtsch. Gesundh.- Wes., 23: 2039-41, 1968.
13. LOWRY, O.H.; ROS0EBROUGH, N. J. ; :FARR, A.L.& RANDALL, R.J. - Protein measurement with the Folin phenol reagen t. J. biol. Chem., 193: 265-75, 1951.
14. MEULEMANS, O. - Determination of total protein in spinal fluid with sulphosalicylic
acid and trichloroacetic acid.
Clin. chim. Acta, 5: 757-61, 1960.
15. PASHLEY, D. H.; SCHUSTER, G. S.; P ALMER, P. & SHARAWY, M.M. - Some drugs and other substances that interfere wíth protein determination. Clin. Chem., 19: 263-65, 1973.
16, PLUM, C.M.; HERMANSEN, L. & PETERSEN, I. - Fractionated protein
determination on small quantities
(C.S.F., urine, serum dilution). Scand. J. clin. Lab. Invest., 7 (Suppl. 18): 3-35, 1955.
17. RICE, E. W . - Total proteins in cerebrospinal fluid CTurbimetric). Stand. Meth. clin. Chem., 2: 231-36, 1965.
18. RICE, E. W. & LOFTIS, J. W. - Critiqueof the determina tion of proteins in cerebrospinal
fluido Evaluation of the biuret method of Goa and the TCA turbidimetric method of Meulemans. Clin. Chem., 8: 56-61, 1962.
19. RIEDER, H. P. - Vergleich einiger Methoden zur Bestimmung des Gesamteiweisses
im Liquor und anderen stark verdünten Lõsungen. Clin. chim. Acta, 3: 455-70, 1958.
20. RIEDER, H. P. - Eine neue Modifikation der Cu-Folin-Methode zur Bestimmung des Total-proteins im Liquor cerebrospinalís, Klin. Wschr., 44: 1036-40, 1966.
21. SETHNA, S. & TSAO, M.U. - Protein level in cerebrospinal fluido Clin. Chem, 3: 249-56, 1957.
22. SPINA-FRANÇA, A. & AMAR, I. - Valoresnormais da concentração protéica do líquido cefalorraquidiano: variações ligadas ao local de colheita da amostra.Arq. Neuro-psiquiat. (S. Paulo). 19: 220-25, 1961.
23. SPINA-FRANÇA, A.; CERQUE IRA, F.M.C. & AMAR, I. - Proteinorraquia. Estudo crítico de métodos de dosagem. Arq. N europsiquiat. (São Paulo), 13: 303-12, 1955.
24. SVENSMARK, O. - Determination of protein in cerebrospinal fluido A comment
on the Lowry method. Scand. J. clin. Lab. Invest., 10: 50-2, 1958.
25. VERMES, L.M.S. - Proteínas totais do líquido cefalorraqueano: métodos de determinação e níveis nonnais (variações ligadas ao sexo, idade e local de punção).
São Paulo, 1975. [Tese - Instituto
de Ciências Biomédicas da Universidade de São Paulo]
26. ZONDAG, H.A. & BOETZELAER, G.L. - Determination of protein in cerebrospinal fluido Sources of error in the Lowry rnethod. Clin. chim. Acta, 5: 155-6, 1960.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 1977 Instituto Adolfo Lutz Journal
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