Keywords
anticorpos anti-L-asparaginase, inibição da atividade catalítica
cromatografia em camada delgada na atividade da L-asparaginase
enzima sérica de eutia
cromatografia em camada delgada na atividade da L-asparaginase
enzima sérica de eutia
How to Cite
1.
Calichi VLG, Ferri RG. Agoutiserum L-asparaginase. III - Antienzymeprecipitatingantibodies. Rev Inst Adolfo Lutz [Internet]. 1977 Dec. 30 [cited 2024 Jul. 22];37(1-2):23-5. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/37096
Abstract
Anti-L-asparaginase precipitating antibodies were studied in ten different immune-sera. It was done by precipitation reactions in liquid media using as antigen an enzyme preparation obtained by partia! puriiication of agouti
serum. Supernatants and precípitates were incubated wi th L-asparagine as a substrate.
In the presence of L-asparaginase, L-asparagine was converted into L-aspartic
acid, and both amino acids were characterized by th in-layer chromatography on
silica-gel. Seven of ten immune sera exhibited high levels of anti-enzyme precipitating
antibodies, and the thrce remaining sera contained low lsvels of these antibodies.
A relationship was not found between the immune-sera protein concentration
and antibody activity.
References
1. BOYSE, E.A.; OLD, L.J.; CAMPBELL, H.A. & MASHBURN, L.T. - Suppression of murine leikemias by L-asparaginase. Incidence of sensitivity among leukemias of various types: comparative
inhibitory activities of guine a pig serum L-asparaginase and Escherichia coZi L-asparaginase. J. ezp. Med., 125: 17-31, 1967.
2. BURCHENAL, H.H.; BENVENISTI, D. & DOLLINGER, M. - Experimental studies with L-asparaginase in mouse leukemias.
Recent Resulte Cancer Res., 33:
102-13, 1970.
3. CALICH, V.L.G. & FERRI, R.G. - L-asparaginase de soro de cutia. I, Caracterização imunoquímíca da preparação enzimática parcialmente purificada. Rev,
Hosp, Clín. Fac. Med. Univ. S.Paulo,
31: 334-8, 1976.
4. CALICH, V.L.G. & FERRI, R.G. - Agouti serum L-asparaginase. n. Chromatographic fractionation and characterization of enzimatically aetive eomponent.
Rev. Inst. Med. trop. S. Paulo, 18:
239-45, 1976.
5. CAPIZZI, R.L.; BERTINO, J.R. & HANDSCHUMACHER, R.E. - L-asparaginase. Ann. Rev. Med., 21: 433-44, 1970.
6. DOHLWITZ, A.; FRAZÉN, S.; HOLMGREEN, A.; KILLANDER, A.; WIDE, L. & AHSTRbM, L. - Studies on antibody formation in patients treated with L-asparaginase. Recent Resulta Cancer Res., 33: 198-203, 1970.
7. DOLOWY, W.C.; HENSON, D.; CORNET, J. & SELLIN, H. - Toxie and antineoplastie effeets of L-asparaginase. Study of miee with Iymphorna and normal monkeys and report of a child with
leukemia. Cancer, 19: 1813-9, 1966.
8. GORNALL, A.G.; BARDAWILL, C.J. & DAVID, M.M.J. - Determination of
serum proteins by means of the biuret reaetion. J. biol. Chem., 177: 751-66, 1949.
9. HILL, J.M.; LOEB, E.; MACLELLAN, A.; KHAN, A. ROBERTS, J., SHIELDS,
W.T. & HILL, N.O. - Response to
highly purified L-asparaginase during therapy of aeute leukernia, Cancer Res., 29: 1574-80, 1969.
10. KIDD, J.G. - Regression on transplanted lymphomas indueed in vivo by means of normal guinea pig serum. I. Course of transplanted caneers of various kinds in miee and rats given guinea pig serum, horse serum or rabbit serum. J. exp. Med., 98: 565-81, 1953.
11. MEISTER, A. - Omega-amidases. In: COLOWICK, S.P. & KAPLAN, N.O., ed. Methods in Enzimology. New York, Academic press, 1955. v. 2: 383-4.
12. OETTGEN, H.F.; OLD, L.J.; BOYSE, E. A.; CAMPBELL, H.A.; PHILIPS, F. S.; CLARKSON, B.D.; TALLAL, L.; LEEPER, R.D.; SCHWARTZ, M.K. & KIM, J. H. - Inhibition of leukemias in man by L-asparaginase. Cancer Res., 27: 2619-31, 1967.
13. OETTGEN, H.F.; STEPHENSON, P.A.; SCHWARTZ, M.K.; LEEPER, R.D.; TALLAL, L.; TAN, C.C.; CLARKSON, B.D.; GOLBEY, R.B.; KRAKOFF, I. H.; KARNOFSKY, D.A.; MURPHY, L.M. & BURCHENAL, J.H. - Toxity of E. coli L-asparaginase in man. Cancer, 25: 253-78, 1970.
14. PETERSON, R.G.; HANDSCHUMACHER, R.E. & MITCHELL, M.S. - Immunologieal
response to L-asparaginase. J.
clin. lnvest., 50: 1080-90, 1971.
15. PHILLIPS, A.W.; BOYD, J.W.; FERGUSON, JR., D.A. & MARUCCI, A.A. - Immunochemieal comparison of L-asparaginases
irom Serratia marcescens
and Escherichia coli. J. Baet., 107:
461-7, 1071.
16. PINSKY, C.M.; MITCHELL, S.; OETTGEN, H.F. & SCHWARTZ, M.K. - Immune reaetion to L-asparaginase in man. Proc, amo Ass. Cancer Res.; 11: 250, 1970.
17. REIS, H.E.; BRAUN, W. & SCHMIDT, C. G. - Antigenicity of Escherichia coli asparaginase. Rev. Ir. Étud. clin. biol., 14: 906-9, 1969.
18. ROBERTS, J.; PRAGER, M. D. & BACHYNSKY, N. - The antitumor actívity oí Escherichia coli L-asparaginase. Cancer Res., 26: 2213-17, 1966.
19. SCHEIN, P. S.; RAKIETEN, N.; GORDON, B.M.; DAVIS, R.D. & RALL, D.P. - The toxieity of Escherichia coli L-asparaginase.
Cancer se«; 29: 426-34, 1969.
20. SULD, H.M. & HERBUT, P.A. - Immunologieal studies on guinea pig serum and liver L-asparaginases. Purifieation
of L-asparaginases by antibody preeipitation. J. biol. Chem., 45: 2802-8, 1970.
21. VADLAMUDI, S; PADARATHSINGH, M.;
WARAVDEKAR, V.S. & GOLDIN, A.
Factors influencing the therapeutie aetivity of L-asparaginase (NSC 109229) in leukemic (L5178Y) miee. Cancer Res., 39: 1467-72, 1970.
22. YAMASHITA, T. & NISHIGAKI, T.
L-asparaginase. 4. Antigenieity of L-asparaginase. Oyo Yakuri (Japan), 4(4): 601-6, 1970 apud Chem. Abstr., 76: 12737c, 1972.
inhibitory activities of guine a pig serum L-asparaginase and Escherichia coZi L-asparaginase. J. ezp. Med., 125: 17-31, 1967.
2. BURCHENAL, H.H.; BENVENISTI, D. & DOLLINGER, M. - Experimental studies with L-asparaginase in mouse leukemias.
Recent Resulte Cancer Res., 33:
102-13, 1970.
3. CALICH, V.L.G. & FERRI, R.G. - L-asparaginase de soro de cutia. I, Caracterização imunoquímíca da preparação enzimática parcialmente purificada. Rev,
Hosp, Clín. Fac. Med. Univ. S.Paulo,
31: 334-8, 1976.
4. CALICH, V.L.G. & FERRI, R.G. - Agouti serum L-asparaginase. n. Chromatographic fractionation and characterization of enzimatically aetive eomponent.
Rev. Inst. Med. trop. S. Paulo, 18:
239-45, 1976.
5. CAPIZZI, R.L.; BERTINO, J.R. & HANDSCHUMACHER, R.E. - L-asparaginase. Ann. Rev. Med., 21: 433-44, 1970.
6. DOHLWITZ, A.; FRAZÉN, S.; HOLMGREEN, A.; KILLANDER, A.; WIDE, L. & AHSTRbM, L. - Studies on antibody formation in patients treated with L-asparaginase. Recent Resulta Cancer Res., 33: 198-203, 1970.
7. DOLOWY, W.C.; HENSON, D.; CORNET, J. & SELLIN, H. - Toxie and antineoplastie effeets of L-asparaginase. Study of miee with Iymphorna and normal monkeys and report of a child with
leukemia. Cancer, 19: 1813-9, 1966.
8. GORNALL, A.G.; BARDAWILL, C.J. & DAVID, M.M.J. - Determination of
serum proteins by means of the biuret reaetion. J. biol. Chem., 177: 751-66, 1949.
9. HILL, J.M.; LOEB, E.; MACLELLAN, A.; KHAN, A. ROBERTS, J., SHIELDS,
W.T. & HILL, N.O. - Response to
highly purified L-asparaginase during therapy of aeute leukernia, Cancer Res., 29: 1574-80, 1969.
10. KIDD, J.G. - Regression on transplanted lymphomas indueed in vivo by means of normal guinea pig serum. I. Course of transplanted caneers of various kinds in miee and rats given guinea pig serum, horse serum or rabbit serum. J. exp. Med., 98: 565-81, 1953.
11. MEISTER, A. - Omega-amidases. In: COLOWICK, S.P. & KAPLAN, N.O., ed. Methods in Enzimology. New York, Academic press, 1955. v. 2: 383-4.
12. OETTGEN, H.F.; OLD, L.J.; BOYSE, E. A.; CAMPBELL, H.A.; PHILIPS, F. S.; CLARKSON, B.D.; TALLAL, L.; LEEPER, R.D.; SCHWARTZ, M.K. & KIM, J. H. - Inhibition of leukemias in man by L-asparaginase. Cancer Res., 27: 2619-31, 1967.
13. OETTGEN, H.F.; STEPHENSON, P.A.; SCHWARTZ, M.K.; LEEPER, R.D.; TALLAL, L.; TAN, C.C.; CLARKSON, B.D.; GOLBEY, R.B.; KRAKOFF, I. H.; KARNOFSKY, D.A.; MURPHY, L.M. & BURCHENAL, J.H. - Toxity of E. coli L-asparaginase in man. Cancer, 25: 253-78, 1970.
14. PETERSON, R.G.; HANDSCHUMACHER, R.E. & MITCHELL, M.S. - Immunologieal
response to L-asparaginase. J.
clin. lnvest., 50: 1080-90, 1971.
15. PHILLIPS, A.W.; BOYD, J.W.; FERGUSON, JR., D.A. & MARUCCI, A.A. - Immunochemieal comparison of L-asparaginases
irom Serratia marcescens
and Escherichia coli. J. Baet., 107:
461-7, 1071.
16. PINSKY, C.M.; MITCHELL, S.; OETTGEN, H.F. & SCHWARTZ, M.K. - Immune reaetion to L-asparaginase in man. Proc, amo Ass. Cancer Res.; 11: 250, 1970.
17. REIS, H.E.; BRAUN, W. & SCHMIDT, C. G. - Antigenicity of Escherichia coli asparaginase. Rev. Ir. Étud. clin. biol., 14: 906-9, 1969.
18. ROBERTS, J.; PRAGER, M. D. & BACHYNSKY, N. - The antitumor actívity oí Escherichia coli L-asparaginase. Cancer Res., 26: 2213-17, 1966.
19. SCHEIN, P. S.; RAKIETEN, N.; GORDON, B.M.; DAVIS, R.D. & RALL, D.P. - The toxieity of Escherichia coli L-asparaginase.
Cancer se«; 29: 426-34, 1969.
20. SULD, H.M. & HERBUT, P.A. - Immunologieal studies on guinea pig serum and liver L-asparaginases. Purifieation
of L-asparaginases by antibody preeipitation. J. biol. Chem., 45: 2802-8, 1970.
21. VADLAMUDI, S; PADARATHSINGH, M.;
WARAVDEKAR, V.S. & GOLDIN, A.
Factors influencing the therapeutie aetivity of L-asparaginase (NSC 109229) in leukemic (L5178Y) miee. Cancer Res., 39: 1467-72, 1970.
22. YAMASHITA, T. & NISHIGAKI, T.
L-asparaginase. 4. Antigenieity of L-asparaginase. Oyo Yakuri (Japan), 4(4): 601-6, 1970 apud Chem. Abstr., 76: 12737c, 1972.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 1977 Instituto Adolfo Lutz Journal
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