Supplementary Files
Keywords
syphilis serodiagnosis, methods
Treponema pallidum
Treponema pallidum
How to Cite
1.
Takeda AK, Barbosa PMC, Barbosa SFC. Comparative study of flocculation, complement-fixation, indirect immunofluorescence and passive microhaemagglutination reactions for syphilis. Rev Inst Adolfo Lutz [Internet]. 2022 Feb. 22 [cited 2024 May 15];39(2):137-44. Available from: https://periodicos.saude.sp.gov.br/RIAL/article/view/37308
Abstract
The possibility of using the passivo microhaemagglutinatíon reaction for Treponema pallidum (MHPTP) as a routine diagnostic procedure is examined. MHPTP was compared with flocculation, complement-fixation and indirect immunofluorescence reactions in 344 sera from blood-bank donors and patients suffering from syphilis and some other diseases, ln the blood-bank donor g roup, the MHPTP agreed 100% with indirect immunofluorescence reaction but not with complemen t fixation or flocculation testo In th e group of patients with clinical diagnosis of syphilis, there was total agreement among the four reactions.
References
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KALOPOULOS, G. & WALSH, J. L. Treponema pallidum haemagglutination test for yaws. Comparison with the TPI and FTA-ABS tests. Br. J. vener. Dis., 48:479-82, 1972.
4. GARNER, M. F.; BACKHOUSE, J. L.; DASKALOPOULOS, G. & WALSH, J. L. The Treponema pallidum haemagglutination (TPHA) test in biological falsepositive and leprosy sera. J. clin. Path., 26 :258-61, 1973.
5. JOHNSTON, N. A. - Treponema pallidum
haemagglutination test for syphilis, Evaluation of a modified micro-method. Br. J. vener. Dis., 48 :474-8, 1972.
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7. KIRÁLY, R. & PREVAN, H. - Evaluation of the T. pallidum haemagglutination (TPHA) test for syphilis on "problem" sera. In : WORLD HEALTH ORGANlZATlON, Genebra, 1973. (WHO working document WHO/VDS/RES/309.)
8. LESINSKl, J.; DUDZlSZ, B.; KADZlEWICZ, E.; KRACH, J. & SZYMCZAK, M. lnvestigations on the specificity of the TPHA test and its value as a screening and verification test in syphilis diagnosis. ln: WORLD HEALTH ORGANlZATION, Genebra, 1974. 18 p. (WHO working document WHOjVDSjRES/74.317)
9. LUGER, A. & SPENDLINGWlMMER, r. - An appraisal of the Treponema pallidum haemagglutination (TPHA) testo ln: WORLD HEALTH ORGANIZATION, Genebra, 1972. (WHO working document WHOjVDSjRESj284)
10. O'NEILL, P.; WARNER, R. W. & NICOL, C. S. - Treponema pallidum haemagglutina tion assay in the routine serodiagnosis of treponemal diseases. Br. J. ve-
ner. Dis., 49 :427-31, 1973.
11. ORGANlZACIóN PANAMERlCANA DE LA SALUD - Manual de reacciones gicas para el diagnostico de Ia Traduzido da edição original em Washington, D.C., OPS, 1966. (Publ cient. n. 144)
12. ORGANlZAClóN PANAMERICANA DE LA SAL UD - Manual de reacciones para el diagnóstico de la sijilis: Traduzido da edição original em inglês. Washington, D.C., OPS, 1975. p. 36. (Publ. cient. n. 311)
13. Ibid. p. 68.
14. RATHLEV, T. - Hemagglutination tests utilizing antigens from pathogenic and apathogenic Treponema pallidum. In : WORLD HEALTH ORGANIZATION, Genebra, 1972. (WHO working document
WHO/VDS/RES/77.65)
15. RATHLEV, T. Hemagglutination test utilizing pathogenic Treponema pallidum for the serodiagnosis of svphilís. Br. J. vener. Dis., 43:181-5, 1967.
16. SEQUEIRA, P. J. L. & ELDRIDGE, A. E. - TreponemaI haemagglutination test. Br. J. vener. Dis., 49 :242-8, 1973.
17. SHORE, R. N. - Hemagglutination tests and
related advances in serodiagnosis of syphilis, Arch. Dermatol., 109:854-7, 1974.
18. TOMIZAWA, T. & KASAMATSU, S. - HemaggIutination test for the diagnosis of
syphilis. A preliminar reporto Jap. J. med. Sci. Biol., 19:305-8, 1966.
19. WADSWORTH, E.; MALTANER, F. & MALTANER, E. - Quantative studies of the eomplemen t fixation reactons with syphílitic serum and tis sue extract: technic of practical quantitative testo J. immun.,
35:217-34, 1938.
2. COX, P. M.; LOGAN, L. C. & NORINS, L. C. Automated quantitative microhemagglutination assay for Treponema pallidum antibodies. Appl. Microbiol., 18 :485-9, 1969.
3. GARNER, M. F.; BACKHOUSE, J. L.; DAS-
KALOPOULOS, G. & WALSH, J. L. Treponema pallidum haemagglutination test for yaws. Comparison with the TPI and FTA-ABS tests. Br. J. vener. Dis., 48:479-82, 1972.
4. GARNER, M. F.; BACKHOUSE, J. L.; DASKALOPOULOS, G. & WALSH, J. L. The Treponema pallidum haemagglutination (TPHA) test in biological falsepositive and leprosy sera. J. clin. Path., 26 :258-61, 1973.
5. JOHNSTON, N. A. - Treponema pallidum
haemagglutination test for syphilis, Evaluation of a modified micro-method. Br. J. vener. Dis., 48 :474-8, 1972.
6. KAFFMAN, R. E.; WElSS, S.; MOORE, J. D. F. & WIESNER, P. J. - Biological false positive serological tests for syphilis among drug addicts. Br. J. vener, Dis.,
50 :350-3, 1974.
7. KIRÁLY, R. & PREVAN, H. - Evaluation of the T. pallidum haemagglutination (TPHA) test for syphilis on "problem" sera. In : WORLD HEALTH ORGANlZATlON, Genebra, 1973. (WHO working document WHO/VDS/RES/309.)
8. LESINSKl, J.; DUDZlSZ, B.; KADZlEWICZ, E.; KRACH, J. & SZYMCZAK, M. lnvestigations on the specificity of the TPHA test and its value as a screening and verification test in syphilis diagnosis. ln: WORLD HEALTH ORGANlZATION, Genebra, 1974. 18 p. (WHO working document WHOjVDSjRES/74.317)
9. LUGER, A. & SPENDLINGWlMMER, r. - An appraisal of the Treponema pallidum haemagglutination (TPHA) testo ln: WORLD HEALTH ORGANIZATION, Genebra, 1972. (WHO working document WHOjVDSjRESj284)
10. O'NEILL, P.; WARNER, R. W. & NICOL, C. S. - Treponema pallidum haemagglutina tion assay in the routine serodiagnosis of treponemal diseases. Br. J. ve-
ner. Dis., 49 :427-31, 1973.
11. ORGANlZACIóN PANAMERlCANA DE LA SALUD - Manual de reacciones gicas para el diagnostico de Ia Traduzido da edição original em Washington, D.C., OPS, 1966. (Publ cient. n. 144)
12. ORGANlZAClóN PANAMERICANA DE LA SAL UD - Manual de reacciones para el diagnóstico de la sijilis: Traduzido da edição original em inglês. Washington, D.C., OPS, 1975. p. 36. (Publ. cient. n. 311)
13. Ibid. p. 68.
14. RATHLEV, T. - Hemagglutination tests utilizing antigens from pathogenic and apathogenic Treponema pallidum. In : WORLD HEALTH ORGANIZATION, Genebra, 1972. (WHO working document
WHO/VDS/RES/77.65)
15. RATHLEV, T. Hemagglutination test utilizing pathogenic Treponema pallidum for the serodiagnosis of svphilís. Br. J. vener. Dis., 43:181-5, 1967.
16. SEQUEIRA, P. J. L. & ELDRIDGE, A. E. - TreponemaI haemagglutination test. Br. J. vener. Dis., 49 :242-8, 1973.
17. SHORE, R. N. - Hemagglutination tests and
related advances in serodiagnosis of syphilis, Arch. Dermatol., 109:854-7, 1974.
18. TOMIZAWA, T. & KASAMATSU, S. - HemaggIutination test for the diagnosis of
syphilis. A preliminar reporto Jap. J. med. Sci. Biol., 19:305-8, 1966.
19. WADSWORTH, E.; MALTANER, F. & MALTANER, E. - Quantative studies of the eomplemen t fixation reactons with syphílitic serum and tis sue extract: technic of practical quantitative testo J. immun.,
35:217-34, 1938.
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