Keywords
Flavivirus
Iguape
Anopheles (Kerteszia) cruzii
Atlantic Rain Forest
How to Cite
Abstract
The flavivirus Iguape was isolated for the first time in 1979, from a sentinel mouse exposed in a forested area in Iguape County - SP, Brazil. Monotypic hemagglutination inhibition antibodies for Iguape virus were detected in domestic and sylvan animals, in Ribeira Valley region, São Paulo State. Monotypic antibodies have also been detected in human sera, although there is no evidence of clinical disease. The authors report the isolation of a new strain of Iguape virus from naturally infected mosquitoes. The study was conducted in May, 1994 in Juquitiba County - São Paulo State, in a forested area. Mosquitoes were captured by light traps. After systematics identification, pools of mosquitoes were processed for virus isolation in suckling mice. An isolate (SPAr-158482) was obtained from Anopheles (Kerteszia) cruzii. Tests of Hemagglutination, Hemagglutination Inhibition, Complement Fixation, Neutralization in suckling mice and sensitivity to Sodium Deoxycholate were employed for identification of the isolate.. According to tests of Complement Fixation, Hemagglutination Inhibition and Neutralization in mice, the virus was identified as the same as Iguape virus. This finding suggests that this species of mosquito may play a role in the transmission cycle of Iguape virus in natural environment.
References
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