Palabras clave
biofilmes
Staphylococcus
Cómo citar
Resumen
Staphylococcus aureus é um dos principais causadores de doenças de origem alimentar e, embora a Agência Nacional de Vigilância Sanitária – ANVISA não contemple especificamente a detecção de estafilococos coagulase-negativa (ECN) em alimentos, sabe-se que este grupo de bactérias apresenta genes responsáveis pela formação de biofilme e produção de enterotoxinas. No presente estudo foram identificados os S. aureus e ECN em amostras de salgados, e sua capacidade de produzir biofilme in vitro
foi avaliada por duas metodologias: ágar vermelho Congo e placa de microtitulação de poliestireno. Vinte e dois isolados de Staphylococcus pertencentes a oito diferentes espécies foram obtidos de 122 salgados, sanduíches e lanches. S. aureus, S. warneri e S. haemolyticus foram as espécies mais frequentemente isoladas. A produção de biofilme foi verificada em sete (31,8%) isolados pela técnica de ágar vermelho Congo e em três (13,6%) em placa de microtitulação. Nenhum isolado foi considerado positivo em ambas as metodologias. Apesar do baixo número de isolados, houve concordância de 59,1% entre os testes. A
capacidade de produzir biofilme é importante fator de virulência para Staphylococcus spp. e a detecção
destes isolados de amostras de alimentos pode ajudar a definir o papel dos ECN como agentes patogênicos
transmitidos por alimentos.
Citas
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